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Articles by Hui Zhang
Total Records ( 17 ) for Hui Zhang
  Jauricque Ursulla Kongo-Dia-Moukala and Hui Zhang
  A number of operating parameters including temperature, pH, solvent/flour ratio and time influence the yield of protein during extraction. In order to effectively identify the good combinations of the extraction conditions, Response Surface Methodology (RSM) was employed to determine optimum conditions for extraction of protein from defatted corn. The coefficient of determination (0.9789) was good for the second-order model. Protein extraction from defatted corn was mainly affected by pH and solvent/flour ratio. From RSM-generated model, the optimum extraction conditions were 54°C, 11.55, 1:18 and 33 min for temperature, pH, solvent/flour ratio and time, respectively. Under these conditions, the experimental protein content was 69.25 mg mL-1 which agreed closely with the predicted value 71.23 mg mL-1. To ensure that the protein extract could be utilized for food application, some functional properties such as water holding capacity, oil holding capacity, emulsifying capacity, foaming capacity and foaming stability were evaluated. Defatted corn protein had a good water holding and foaming capacity. Thus, it can be used in food formulation systems.
  Jauricque Ursulla Kongo-Dia-Moukala , John Nsor-Atindana and Hui Zhang
  Enzymatic hydrolysis of plant protein is used as a source of bioactive peptides. In the present study, defatted corn protein was treated with Flavourzyme to produce hydrolysate with hypocholesterolemic activity for potential application in functional food. Defatted corn protein was prepared by an alkaline method and used as a substrate for enzyme hydrolysis. The derived hydrolysate was assessed for their hypocholesterolemic activity in different in vitro assay systems, including cholesterol micellar solubility inhibition and bile acids binding capacities. The bile acids used were: sodium glycocholate, sodium cholate and sodium deoxycholate. To know much about the defatted corn hydrolysate, the solubility, hydrophobicity and effect of amino acids content of hydrolysate on hypocholesterolemic activity were investigated. Hydrolysis with Flavourzyme for 90 min yielded a DH of 12.25%. The results showed that defatted corn hydrolysate had effective hypocholesterolemic activity. Hydrolysate had 67.42, 19.01, 9.99 and 86.90% of cholesterol micellar solubility inhibition, sodium glycocholate, sodium cholate and sodium deoxycholate, respectively. The amino acid analysis revealed that the predominant hydrophobic amino acids residue were Leucine, Valine, Alanine, Proline, Glycin and Phenylalanine. This result suggested that defatted corn protein hydrolysate could exhibit a hypocholesterolemic activity.
  Hui Zhang , Qiong Li , Haibin Zhang and Xiamu Niu
  This study proposed a novel benchmark for evaluating the robustness and discriminability properties of perceptual hashing algorithms. Firstly, two major problems neglected by traditional benchmark are analyzed thoroughly with a concrete experiment. One problem is the inconsistence between the subjective feeling and the objective perceptual distance, the other is the partiality of the performance for different attacks. And then, in order to overcome the problems, a new benchmark for perceptual hashing based on human subjective identification is proposed and the corresponding evaluation methods are presented by illustrative experiments and examples. Present benchmark methods are fairer and more comprehensive than the traditional methods.
  Hui Zhang , JiFeng Zhang , Ran Huang and QingHua Wang
  The existing Web services integration systems have three major disadvantages, in order to improve these disadvantages, this study propose extend the traditional contract net model so as to improve its disadvantage in allocating tasks. In the extended model, the tendering tactic is developed based on creditworthiness of task acquaintance and the bid awarded tactic is advanced based on the fuzzy comprehensive evaluation. Hence, it not only guarantees the quality of task fulfillment but also solves the problem of excessive communication in the traditional contract net model.
  Yan Qiang , Yue Li , Wei Wei , Bo Pei , Juanjuan Zhao and Hui Zhang
  In order to guarantee the stability of the system performance and the high Qos(quality of service) of users, a new method based on the HDFS (Hadoop Distributed File System) was proposed which including a job type classification method and a dynamic replica manage mechanism. The method uses the job type classification method to select the I/O intensive job, in order to achieve more accuracy of the classification taken the heterogeneity of the jobs into consideration. For the classified jobs, a dynamic replica manage mechanism was used to determine whether to increase or decrease the number of copies on the specific data node. For a test of a cluster with 1 namenode and 20 data nodes, the method has a high performance. The theoretical and experimental analyses in this paper prove that the proposed method has the ability to improve the performance of HDFS effectively.
  Li Wang , Hua-Neng Xu , Huiyuan Yao and Hui Zhang
  The water, ethanol, and methanol extracts of Vaccinium Bracteatum Thunb. leaves (VBTL) were investigated for their potential antioxidant activity. The total phenolic content was determined, and the antioxidant activity of the extracts was assessed by 1, 1–diphenyl–2–picrylhydrazyl (DPPH) radical scavenging analysis. Five phenolic compounds including quercetin, kaempferol, luteolin, apigenin, and chrysin, were identified in the ethanol extract by high pressure liquid chromatography (HPLC).
  Jinliang Sheng , Chuangfu Chen , Xia Yang , Yuanzhi Wang , Pengyan Wang and Hui Zhang
  In this study, Real-Time Reverse-Transcription Polymerase Chain Reaction (RT-PCR) assays were performed with EvaGreen to investigate the dynamics of cytokine (Interleukin (IL)-1β, IL-8, Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) and Interferon (IFN) (γ) and Toll-Like Receptor 2/4 (TLR2/4) gene expression in ovine primary Alveolar Macrophages (AMs) following Lipopolysaccharide (LPS) stimulation. Expression of cytokine and TLR2/4 mRNA was quantified by comparison of Cycle threshold (CT) values with a standard curve generated from plasmid DNA containing the target gene. Examination of LPS-stimulated ovine AMs revealed that cytokine mRNA expression peaked between 4 and 12 h with the exception of IFN-γ mRNA which peaked around 16 h post stimulation. Furthermore, TLR2 and TLR4 mRNA expression rapidly increased post-stimulation and peaked 20 min post-stimulation at a level which was maintained throughout the procedure. In summary, a sensitive and reliable real-time RT-PCR protocol was implemented for the analysis of ovine TLR2/4 and cytokine gene expression profiles.
  Fei Guo , Yuanzhi Wang , Chuangfu Chen , Hui Zhang , Jun Qiao , Yan Ren , Junbo Zhang and Zhiqiang Li
  TFSS is an important virulence factor of Brucella, organized as one operon containing 12 different across the cell wall bacterial proteins among which VirB5 regulates the host phagocytosis of Brucella and the transportation of Brucella in the host cells. This study has constructed cDNA library from Brucella melitensis 16M-infected murine macrophage Raw264.7, identified and confirmed the interaction between Brucella VirB5 and FTH1 of RAW264.7 using yeast two-hybrid and Co-Immunoprecipitation (Co-IP) technologies. Subsequently, the morphological changes and the expression of apoptosis-related genes in Brucella-infected RAW264.7 cells have been investigated with Electron Microscope (EM) and real-time quantitative RT-PCR, respectively. The present study has demonstrated that VirB5 and FTH1 play important roles in intracellular parasitism of Brucella and inhibition of FHT1 expression accelerates the apoptosis of macrophage.
  Wei Chen , Jinghua Ma , Hui Zhang , Dan Li and Xianfu Zhang
  The Red-crowned crane (Grus japonensis) is one of the largest birds in East Asia which is among the rarest and endangered cranes in the world. In order to enhance population management and further conservation of Red-crowned cranes, researchers compared behavioural changes from wild population to captive population. Meanwhile, researchers also compared the artificial inbred population with the natural normal populations. In this research, five main behavioural patterns of Red-crowned cranes include resting, moving, preening, feeding and alerting were identified by all occurrence sampling and instantaneous scanning sampling methods with 5-10 min intervals. The referred wild and captive populations were concluded from the previous publications and the artificial inbred populations were observed in Hangzhou Wildlife Park (China) during August and September, 2009. As a result, researchers found the difference (p>0.05) between wild and captive populations is not significant. However, preening in captive population is higher than in wild population. The wild population spent more time for resting compared to captive population yet feeding is the most time-spent behaviours for both populations. Furthermore, behavioural patterns between normal and inbred populations are found significantly different (p<0.05). Alerting and resting behaviours are significantly higher in the normal population than in inbred population. Moreover, moving is the dominant behaviour of inbred population but feeding is taken the most time by normal population.
  Lihua Tang , Hongling Wang , Yun Luo , Lijun Huang , Hongxia Gong , Hui Zhang and Jianyue Wang
  V. parahaemolyticus is one of the most important foodborne pathogens in China and other countries. In this study, researchers investigated the contamination of V. parahaemolyticus in shellfish during different seasons in Zhoushan islands. Meanwhile, researchers reported the prevalence, molecular characterization and virulence genes of V. parahaemolyticus from shellfish and clinical patients. In shellfish, the positive rate was 85, 80, 75 and 10%, respectively in different seasons from Spring to Winter. Among the shellfish isolates, there was no tdh+ strains and only 4% trh+ strains. The prevalence of V. parahaemolyticus in clinical samples was 36 and 77.78% of clinical strains were tdh+, toxRS/new+, orf8+ and 5.56% clinical strains were trh+. In 80% similarity, the total of 53 strains could be divided into twenty nine types. Most of the shellfish strains present various patterns with far relationship while all the pandemic strains from clinical samples belonged to same clone group with 82.8% similarity. The tdh+, toxRS/new+ and orf8+ strains were the main epidemic strains in Zhoushan islands.
  Jingyu Liu , Suwen Zhang , Qi Pei , Jinna Di , Hui Zhang , Bo Hu and Hao Ai
  Tuberculosis remains a major infectious disease worldwide due to the low efficacy of available vaccine of the Mycobacterium bovis Bacillus Calmette-Guerin (BCG). There is a need to develop protective vaccines against Tuberculosis (TB) that elicit full immune responses including mucosal immunity. Here, a live attenuated salmonella typhimurium aroA SL7207 vector TB vaccine, namely SL (E6-HspX), harboring the Mycobacterium tuberculosis (Mtb) H37Rv ESAT6-HspX fusion gene was developed. Its immunogenicity and protective efficacy were assessed in a mouse model of tuberculosis. Vaccination with the SL (E6-HspX) significantly increased the frequency of peripheral blood CD4+ and CD8+ T cells and induced significantly higher levels of cell-mediated immune response compared with vaccination with PBS or the pVAX1 vector. Vaccination with the SL (E6-HspX) also induced the strongest TB Ag-specific mucosal, humoral responses and exerted high protective efficacy in mice against virulent Mtb H37Rv challenge compared to the other vaccinated groups (mice immunized with SL (HspX) or BCG only). This strategy may represent a novel promising mucosal vaccine candidate for the prevention of TB and may be used for the prevention and therapeutic intervention of Mtb infection.
  Yuanzhi Wang , Ke Zhang , Yali Zhang , Hui Wang , Fei Guo , Lin Zhang , Hui Zhang , Lijuan CaoBuyun , Cui , Chengyao Li , Li Yuan , Wanjiang Zhang , Ze Xu and Chuangfu Chen
  An outbreak of brucellosis occurred in students on field practice at sheep farm in 2005 at Shihezi, Xinjiang Province, the North-West of China. Five of 7 (71.4%) students were seropositive, showing titers ≥1:160 IU mL-1 in STAT and diagnosed as acute brucellosis with physical examination. To characterize Brucella isolates from the outbreak, the research including face to face investigation, Brucella isolation, multiple locus VNTR-16 analysis (MLVA-16) and genome sequencing were carried out. The investigation showed 42.5% (1,293/3,042) of ewes are sero-positive with RBPT and almost half of ewes aborted. Although, no bacteria were isolated from student blood samples, five individual colonies were isolated from aborted sheep fetuses and were identified as B. melitensis biovar 3 by conventional microbiological tests. MLVA-16 typing indicated that the isolates were clustered in the East Mediterranean with genotype 42. They were similar to wild strains from Guangdong in 2008 and Inner Mongolia in 1994 and 1995. They were most close to strain bru0261 from Pakistan student studying in Germany. Genome sequence and phylogenomic tree showed that pathogen in this study was close to Chinese wild and vaccine strains such as B. melitensis M28, M5-90. Researchers first report pathogens isolated in 1980 and 2005 are genotype 42 containing novel MLVA-16 patterns (1-5-3-13-2-2-3-2-4-20-8-8-4-3-7-7) compared to that both in China and other countries.
  Fei Guo , Junbo Zhang , Chuangfu Chen , Yuanzhi Wang and Hui Zhang
  Brucellosis is an infectious disease that brings great economic burdens for developing countries. The vaccine S2 which is an attenuated Brucella suis (B. suis) strain has been used on a large scale in China. However, the immunity induced by S2 declined relative to those vaccinated with Rev-1 and S19 vaccines. Moreover, the vaccine S2 cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent B. suis vaccine is needed. In this study, a vjbR mutant of B. suis (B. suisΔvjbR) was constructed overcome these drawbacks. The B. suisΔvjbR strain showed reduced survival capability in RAW264.7 macrophage and mice and induced high protective immunity in mice. In addition, B. suisΔvjbR induced an anti-Brucella-specific IgG (immunoglobulin G) response and stimulated the expression of gamma interferon (INFγ). Further, the vjbR antigen allowed serological differentiation between natural and vaccinated infection in mice. Therefore, B. suisΔvjbR was suggested as a safe and efficacious live vaccine candidate against virulent Brucella suis (B. suis) infection.
  Hui Zhang and Myunghee Cho Paik
  In longitudinal studies, missingness of data is often unavoidable. Valid estimators from the generalized linear mixed model usually rely on the correct specification of the missing data mechanism. An incorrectly specified missing mechanism may lead to a biased estimator. In this article, we propose a class of unbiased estimating equations using pairwise conditional technique to deal with the generalized linear mixed model under benign non-ignorable missingness where specification of the missing model is not needed. We show that the proposed estimator is consistent and asymptotically normal under certain conditions. Simulation results and an example using longitudinal course of neuropsychological data are also shown.
  Li-Peng Wu , Xi Wang , Lian Li , Ying Zhao , Shaoli Lu , Yu Yu , Wen Zhou , Xiangyu Liu , Jing Yang , Zhixin Zheng , Hui Zhang , Jingnan Feng , Yang Yang , Haiying Wang and Wei-Guo Zhu
  Histone deacetylase inhibitor (HDACi) has been shown to demethylate the mammalian genome, which further strengthens the concept that DNA methylation and histone modifications interact in regulation of gene expression. Here, we report that an HDAC inhibitor, depsipeptide, exhibited significant demethylating activity on the promoters of several genes, including p16, SALL3, and GATA4 in human lung cancer cell lines H719 and H23, colon cancer cell line HT-29, and pancreatic cancer cell line PANC1. Although expression of DNA methyltransferase 1 (DNMT1) was not affected by depsipeptide, a decrease in binding of DNMT1 to the promoter of these genes played a dominant role in depsipeptide-induced demethylation and reactivation. Depsipeptide also suppressed expression of histone methyltransferases G9A and SUV39H1, which in turn resulted in a decrease of di- and trimethylated H3K9 around these genes` promoter. Furthermore, both loading of heterochromatin-associated protein 1 (HP1α and HP1β) to methylated H3K9 and binding of DNMT1 to these genes` promoter were significantly reduced in depsipeptide-treated cells. Similar DNA demethylation was induced by another HDAC inhibitor, apicidin, but not by trichostatin A. Our data describe a novel mechanism of HDACi-mediated DNA demethylation via suppression of histone methyltransferases and reduced recruitment of HP1 and DNMT1 to the genes` promoter.
  Hui Zhang , Li Chen , SiFeng Zhao , Juan Ren and XiaoLei Cao
  Bacillus subtilis S44 is a promising plant growth-promoting rhizobacterium and a potent biocontrol agent isolated from cotton rhizosphere. The ituD gene was cloned and identified from S44. A suicide vector containing ituD gene from B. subtilis S44 was recombined into the B. subtilis S44 chromosome to disrupt the ituD gene. Recombination was demonstrated by PCR, antifungal activity and HPLC confirmed the loss of the ituD gene. The growth rate of the recombinant is severely depressed compared with wild type and tetracycline had no effect on the growth rate the mutant strains. Antifungal activity in cell-free extracts of the recombinant has almost no antifungal activity compared with the wild strain. HPLC analysis show that the wild strian produce iturin A at an amount of 89.4 μg mL-1 while the ituD mutant only 3.41 μg mL-1. In pot and plot tests, the control effects of S44 treatment were 80.30 and 72.97%, respectively. The mutant strains S1 and S2 exhibit no control effect of cotton Rhizoctoniosis. These results suggest that the ituD gene was important for iturin A synthesis and biocontrol activity of S44.
  Qingling Li , Hui Zhang , Yan Wang , Bo Tang , Xin Liu and Xiaocong Gong
  A versatile programmable eight-path-electrode power supply (PEPS) system for manipulating microfluids of a complex microfluidic chip has been developed. The PEPS system consisted of a single chip microprocessor as a central control unit and a personal computer (PC) as an upper computer, and the program could be operated under Windows98/2000/XP. The voltage output of each electrode was in the range of 0 to +8000 V (0.1% precision) while the current output was in the range of 0 to +999 μA. The voltage of eight electrodes could be operated either independently or synchronously by random combination of any electrodes through switching. The voltage output modes were “switch-off/floating”, “switch-on” and “grounded” and fast switched at ms-level between these modes, and run time (0.1 s precision) of these modes could be controlled as desired. The PEPS system was conveniently for controlling flow rate and direction of electroosmotic flow (EOF) in a chip network. Six electrodes were chosen to control the repeated ‘injection and separation’ of 1.0 x 10–5 M fluorescein isothiocyanate (FITC) in a six-reservoir glass-based chip. The relative standard deviation (R.S.D., n = 4, S/N = 10) of the repeated operation was 0.9% for the reservation time (tR) and 2.3% for the peak height, respectively.
 
 
 
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