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Articles by Zhichao Zhang
Total Records ( 2 ) for Zhichao Zhang
  Huadong Yin , Zhichao Zhang , Xi Lan , Xiaoling Zhao , Yan Wang and Qing Zhu
  The MyoD gene family has been proposed to profoundly modulate muscle development and carcass performance in farm animals. In this study, researchers examined Single Nucleotide Polymorphisms (SNPs) in the exons of the Myf5, Myf6 and MyoG genes using Polymerase Chain Reaction (PCR)-Single Strand Conformation Polymorphisms (SSCP) and DNA sequencing methods in 360 individuals from 6 commercial pure lines of Sichuan Daheng meat type quality chickens. About 2 SNPs (87T>C and 96C>T) in exon 1 of Myf5, 1 SNP (154T>C) in exon 1 of MyoG and no variation in Myf6 were detected. The 96C>T SNP in Myf5 was a rare variant and was not analyzed further. The association analysis of genotypes with carcass traits revealed that the genotypes of SNP (87T>C) in Myf5 were significantly associated with Live Weight (LW), Carcass Weight (CW), Semi-Eviscerated Weight (SEW) and Eviscerated Weight (EW) (p<0.05). The SNP genotypes (154T>C) in MyoG were significantly associated with Live Weight (LW), Carcass Weight (CW), Eviscerated Weight (EW) and Breast Muscle Weight (BMW) (p<0.05). The results suggested that Myf5 and MyoG genes are potential major genes or are in close linkage disequilibrium with the QTL affecting carcass traits in this population of chickens. The 2 SNPs may potentially have use as markers for Marker-Assisted Selection (MAS) in chicken breeding.
  Huadong Yin , Yan Wang , Xiaoling Zhao , Shiyi Chen , Zhichao Zhang , Yiping Liu and Qing Zhu
  Fatty deposition in organism is an important factor which influences meat quality. Previously, Heart Fatty Acid-Binding Protein (H-FABP) gene was studied as a candidate gene which having effect on Intramuscular Fat content (IMF). In current study, we chose four SNPs loci (g.260T>C, g.675G>A, g.783C>T and g.1198T>C) of H-FABP which have been proved to have distinct correlation with IMF as molecular markers to scan SNPs in a new breeding Chinese yellow-feather broiler population. Researchers allocated adaptive individuals into high-IMF group and low-IMF group according to the haplotypes which were constructed of genotypes at four SNP sites of each individual in F1 generation and these haplotypes had been proved to be the preponderant or recessive haplotype. The core group was chose from the F1 generation based on the traditional breeding method. And then, we detected the genotypes of the four loci by SNPs screening and analyzed the selection effect in F2 generation which were obtained from mating and crossing within each group. Results indicated that the preponderance alleles were changed at Locus 1, 3 and 4 in the three groups of F2 and there were no significant differences in IMF among the three groups (p>0.05). However, the IMF of the core group and the low fat group was higher than which of high fat group. The result of variance analysis showed that there were no significant difference in the effect of the different genotypes of the four SNPs on IMF (p>0.05). The four loci on the H-FABP gene in the research were not suitable as candidate markers to select IMF in this yellow-feather broiler strain.
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