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Articles by Amany H. Aboellil
Total Records ( 2 ) for Amany H. Aboellil
  Amany H. Aboellil and Neveen. S. Geweely
  The treatment of straws from different plants for a long time up to 60 days could lead to an enrichment of organisms which have xylanases with high pH optima. The isolated fungi from ammonia treated-rice staw and sugar cane bagasse recorded 366 colonies constituting 10 species, while sugar can bagasse isolates reached 215 colonies representing 7 species. Sugar can bagasse as an isolation source possessed higher fungal diversity than rice straw. Mesophilic fungi were dominant in either rice staw or sugar can bagasse sources followed by the thermophilic fungi, which were isolated rarely. Scopulariopsis brevicaulis highly dominated straw and bagasse. Thermophilic Aspergillus versicolor and mesophilic Scopulariopsis brumptii dominated bagasse, whereas thermophilic Aspergillus terreus and mesophilic Acremonium strictum were encountered from straw.
  Amany H. Aboellil
  The efficacy of Trilogy, a natural product from neem (Azadirachta indica Fuss.), against cucumber (Cucumis sativus L.) powdery mildew (Podosphaera xanthii) was studied in detached leaf and intact plant experiments. Trilogy significantly retarded several growth parameters of the pathogen, viz. multiple germ tube formation, number of germ tubes, haustoria and colony size on cucumber leaves. Trilogy induced hypersensitive reaction (HR), as evidence by browning of host cell associated with appressoria. Furthermore, increase in protein concentration of intercellular fluid followed treatment with Trilogy. The elicited leaves exhibited significantly high activity of enzymes: Phenylalanine Ammonia Lyase (PAL) and Tyrosine Ammonia Lyase (TAL) along with rapid and distinct accumulation of fungitoxic phenolic compounds (phytoalexin). Based on analysis of foliar tissues, at least five separate phenolic compounds were identified as intrinsic components of cucumber plants. The pathogen P. xanthii alone induced the production of other seven identifiable phenolic compounds within the leaves of the cucumber plants. These compounds, displayed a significant increase in concentration as a result of elicitation with Trilogy when the plant was stressed by the pathogen especially cucumerin A and B. The combined amount of these antifungal compounds in treated plants was nearly five times the level found in control plants. Phytoalexin production was triggered by the combination of an eliciting/inoculation treatment. These results provide direct evidence that Trilogy induced resistance in cucumber associated with increased extractable enzymatic activity. Accumulation of flavonoid compounds response to an eliciting treatment after infection creates incompatible interactions with powdery mildew.
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