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The physicochemical properties (hydrophobicity, electron donor/electron acceptor properties (acid-base) of three E. coli strains have been investigated using contact angle measurements. Two conditions of culture were used: The cells were grown in Liquid Luria Bertani medium (LLB) and in Solid Luria Bertani medium (SLB). Based on free energy of interactions (ΔGiwi), we found that all E. coli strains have hydrophilic character. Moreover, the cell surface of E. coli strains is predominately electron donor and weakly electron acceptor. The relationships between electron donor/electron acceptor (acid-base) properties and surface chemical composition determined by X-ray Photoelectron Spectroscopy (XPS) were investigated. We show that the phosphate groups play the crucial role in determining the electron donor (base) property and the amine groups were responsible for decreasing the electron acceptor (acid) property. The relation between surface molecular compositions determined by modeling the XPS data and the electron donor/electron acceptors properties were also examined. High concentration of polysaccharides seems to be responsible for high electron acceptor (acid) property. Moreover, the ratio of polysaccharides/proteins could be an origin of the electron acceptor property of studied cells surface.