Kazrin F is involved in apoptosis and interacts with BAX and ARC

Q Wang, M Liu, X Li, L Chen and H. Tang

Acta Biochimica et Biophysica Sinica, 2009, 41(9), 763-772. DOI: 10.1093/abbs/gmp065

Abstract

Kazrin has recently been identified as a functional protein that is involved in cell–cell junctions and in signal transduction. Here, we identified a new isoform, Kazrin F, which is 518 aa in length and has 97 aa unique at the N-terminus. Knockdown of Kazrin F using siRNA caused cell apoptosis and a marked decrease in cell viability measured by MTT and TUNEL assays. Co-immunoprecipitation analysis revealed that Kazrin F interacts with ARC (apoptosis repressor with caspase recruitment domain) and Bax (Bcl-2-associated X protein). Co-localization of Kazrin F with ARC and Bax in the cytoplasm was determined by immunofluorescence analysis. These results suggested that Kazrin F might play an important role in regulating cellular apoptosis by interacting with ARC and Bax.

ASCI-ID: 1071-73