Research Article
Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology

Rozita Omar, M.A. Abdullah, M.A. Hasan and M. Marziah

American Journal of Applied Sciences, 2004, 1(3), 215-219.

Abstract

The effects of sucrose, indole-3-acetic acid (IAA) and 6-benzylaminopurine (BAP) concentrations on cell growth of Centella Asiatica cell suspension culture were studied. The concentrations were designed using Central-composite experimental design and regression analysis was carried out to obtain response surface model describing cell growth for prediction of optimum conditions. Only sucrose as a single factor was positively significant on cell growth. Increasing sucrose concentration from 3.32 to 6.68% (w/v) resulted in an increase in dry cell weight from 16 to 27 g L^-1. IAA and BAP as single factors and other possible interactions effect were insignificant. The optimum values predicted were 6.68% (w/v) sucrose, 0.84 mg L^-1 IAA and 1.17 mg L^-1 BAP yielding 27.4 g L^-1 dry cell weight with 81.4% regression equation fitness to the experimental data.

ASCI-ID: 2809-913