Research Article
The Muscle Transcription Factor MyoD Regulates the Expression of Hepatocyte Growth Factor during the Process of Muscle Development

F. Liu, J. Fei, N. Li and Q. Meng

Asian Journal of Animal and Veterinary Advances, 2010, 5(8), 557-565.


Muscle regeneration recapitulates myogenesis. HGF (hepatocyte growth factor) plays an important role in muscle development, as the only secreted growth factor, which can activates myoblasts proliferation. The expression units of wild HGF promoter-luciferase vector exceeded the expression units of HGF promoter-luciferase vector with mutated binding sequence of MyoD, also the expression trend of HGF was in accordance with the expression of MyoD. The expression of MyoD directly affects the expression of HGF during muscle development. In C2C12 culture, the expression of HGF is up-regulated in the phase of proliferation process, but down-related in the phase of differentiation. We identified the proliferation and differentiation stage of C2C12 culture by the definition of expression of myogenic regulator family (MyoD, yf5, yogenin, Myf6) and detected the HGF level during this two stages. MyoD bind site was found in the 5’-UTR of HGF promoter. MyoD is b HLH (basic helix-loop-helix) transcriptional activator which has a critical role during myogenesis. We verified that MyoD can bind the active b HLH site, as shown by EMSA. Transfection of wild HGF promoter-luciferase eukaryotic expression vector with MyoD binding site and HGF promoter-luciferase eukaryotic expression vector with mutated MyoD binding site into C2C12 myoblasts was done.

ASCI-ID: 13-269

Cited References Fulltext

Cited By

Phylogenetic and Evolutionary Analyses of the Animal Hairy and Enhancer of Split Basic Helix-loop-helix Transcription Factors

Asian Journal of Animal and Veterinary Advances, 2012, 7(5), 390. DOI: 10.3923/ajava.2012.390.402

SNPs of bovine HGF gene and their association with growth traits in Nanyang cattle

Research in Veterinary Science, 2013, (), . DOI: 10.1016/j.rvsc.2013.04.007

Reproductive biology of the endemic and threatened Menderes Nase, Chondrostoma meandrense Elvira, 1987, in Western Anatolia (Osteichthyes: Cyprinidae)

Zoology in the Middle East, 2009, 46(1), 61. DOI: 10.1080/09397140.2009.10638329

AREION: Software effort estimation based on multiple regressions with adaptive recursive data partitioning

Information and Software Technology, 2013, (), . DOI: 10.1016/j.infsof.2013.03.007

Effects of medetomidine and medetomidine-butorphanol on color-coded duplex ultrasonography in healthy dogs

Research in Veterinary Science, 2013, (), . DOI: 10.1016/j.rvsc.2013.04.010

Changed substrate preferences shown by Fringe-toed Lizards, Acanthodactylus scutellatus, from Kuwait’s Al-Burgan oil field (Reptilia: Lacertidae)

Zoology in the Middle East, 2009, 46(1), 41. DOI: 10.1080/09397140.2009.10638326

Macadamia (Macadamia integrifolia) shell and castor (Rícinos communis) oil based sustainable particleboard: A comparison of its properties with conventional wood based particleboard

Materials & Design, 2013, 50(), 117. DOI: 10.1016/j.matdes.2013.03.008