Research Article 
On the Benefit of Pure Glycyrrhizic Acid on the Function and Metabolic Activity of Isolated Pancreatic Langerhans Islets in vitro
Mahban Rahimifard, Mona Navaii-Nigjeh, Amir Nilli-Ahmadabadi, Nazila Pourkhalili, Maryam Baeeri, Azadeh Mohammadirad and Mohammad Abdollahi
Asian Journal of Animal and Veterinary Advances, 2012, 7(11), 1212-1218.
Abstract
Glycyrrhizic Acid (GA) a major component of licorice, has been reported to have potent antioxidant effects and used widely throughout the world. In the present study, the effects of GA on the function, viability and level of Reactive Oxygen Species (ROS) in isolated rat pancreatic islets were evaluated. After Laparotomy, pancreas was removed and islets were isolated and incubated in RPMI 1640 for 24 h and then islets were separated. GA at logarithmic doses (1, 10, 100 and 1000 μM) were added to islets and incubated for 24 h and then static insulin secretion was tested. Also, viability of cells and their ROS level were determined using Mitochondrial Toxicity Test (MTT) and fluorometric assay. Then islets were stained by dithizone and observed under microscope. The results of MTT test indicated that rang of 1-100 μM of GA is safe. In the dose of 1000 μM, GA increased ROS and reduced viability of islets. GA at 1 μM significantly increased secretion of insulin via isolated islets in the presence of stimulation level of glucose (16.7 mM). Results of dithizone staining showed a reduction in live cells at high dose of GA. The LC50 study was done to determine the toxicity of GA on rat pancreatic islets and a 24 h LC50 of 15 mM was found. GA showed remarkable anti oxidative effects at low doses and improved islet’s viability and insulin secretion in stimulation level of glucose. Interestingly, high dose of GA induced oxidative stress and reduced function of islets. The results of the present study indicate that GA is a good candidate to be examined in islet transplantation procedures to maintain islets viable and functional.
ASCI-ID: 13-524
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