Research Article
Incubation and Hatching of Tachypleus gigas (Muller, 1785) Eggs in Sand and Water Media

M.I.M. Faizul, M.M. Faizal, A. Christianus and S.M.N. Amin

Asian Journal of Animal and Veterinary Advances, 2013, 8(2), 333-340.

Abstract

In order to develop suitable methods to successfully hatch Tachypleus gigas eggs, a study was carried out on different salinity and culture media. The main objective for this study was to determine the effect of watering frequency, salinity and media on the incubation period and hatching of T. gigas eggs. This research consisted of three experimental studies. In the first experiment, effect of water salinities (15, 20, 25 and 30 ppt) for watering or moistening sand was studied. For the second study, effect of watering frequencies (once in 1, 3 and 6 days) on the eggs incubated in sand were investigated. As for the last experiment, effect of incubation medium (water and sand) on eggs were compared. Data collected for these experiments were eggs diameter and hatching percentages. Embryonic developments were observed and photographed during the study period. Results from experiment 1, showed that at the end of the incubation period, watering with water salinity of 25-30 ppt produced significantly larger eggs diameter (p<0.05) while percentages of hatching was the highest with 30 ppt water. In experiment 2, it was found that percentages of hatching were significantly higher (p<0.05) when watered once a day and in three days. As for experiment 3, at the end of the incubation period, there was no significant different (p>0.05) in the eggs diameter and percentage of hatching between sand and water medium. In conclusion, the most suitable salinity and watering frequency were 25-30 ppt and once in 3 days, respectively. However, both sand and water are suitable media to successfully incubate T. gigas eggs. Overall, this study showed that T. gigas eggs can hatch as early as 40 days after fertilization.

ASCI-ID: 13-554

Cited References Fulltext

Similar Articles


Blood Cell Histology of Horseshoe Crab, Tachypleus gigas

Asian Journal of Animal and Veterinary Advances, 2013, 8(2), 275-283.

Assessment of Salmonella enteritidis Viability in Egg White during Early Incubation Stages by Fluorescent Staining Method

Asian Journal of Animal and Veterinary Advances, 2012, 7(7), 556-567.

Effect of Exposure to Extremely Low Frequency Electromagnetic Fields (50 Hz, 0.5 mT) During Incubation on Hatchability, T3 and T4 Levels in Newly-hatched Chicks

Asian Journal of Animal and Veterinary Advances, 2012, 7(8), 733-738.

Genetic Diversity of Horseshoe Crab (Tachypleus gigas) in Malaysia Revealed using Microsatellite Markers

Asian Journal of Animal and Veterinary Advances, 2013, 8(1), 63-72.

Blood Cell Histology of Horseshoe Crab, Tachypleus gigas

Asian Journal of Animal and Veterinary Advances, 2013, 8(2), 275-283.