Research Article 
Identification and Phylogenetic Analysis of Peste des Petits Ruminants (PPR) Virus Isolates from India
Dharmender Singh, Yashpal Singh Malik, Kuldeep Sharma and Kuldeep Dhama
Asian Journal of Animal and Veterinary Advances, 2015, 10(8), 386-393.
Abstract
In this study we performed the sequence and phylogenetic analysis of peste des petits ruminants (PPR) virus isolates recovered from natural disease outbreaks in goats of central India. A total of 58 clinical samples comprised of blood, nasal/oral/rectal swabs were collected from 3 natural PPR disease outbreaks. The samples were tested by using N protein based MAb based sandwich ELISA (sELISA) and subsequently RT-PCR targeting Fusion (F) and Nucleoprotein (N) genes of PPR virus. The F and N gene specific PCR amplicons were sequenced and assessed for sequence variations with existing and circulating PPR virus strains and vaccine isolates. The results of this study presents PPR virus antigen in 48.27% clinical samples (28/58) using sELISA confirming this outbreak of PPR in goats. Amplification with primers F1/F2 for F gene yielded an expected amplicon of 372 bp and N1/N2 primer sets yielded an amplicon of 463 bp for N gene in reference vaccine virus as well as 28 field samples. In phylogeny, different patterns of branching based on N and F gene sequences and clustering of many of the central Indian PPR virus isolates with isolates from bordering countries was seen. The results confirm that heterogeneous population of PPR virus isolates is circulating in India which moreover emphasize the significance of molecular methods to understand the epidemiology and diversity of PPR virus in the country. The information from such studies will help in achieving the target of controlling and eradicating the disease, especially when the country is planning to launch the control programs in many states after the successful stamping out of the Rinderpest.
ASCI-ID: 13-752
Similar Articles
Prevalence and Dynamics of Some Gastrointestinal Parasites of Sheep and Goats in Tulus Area Based on Post-Mortem Examination
Asian Journal of Animal and Veterinary Advances, 2008, 3(6), 390-399.
Viral Zoonosis: A Comprehensive ReviewAsian Journal of Animal and Veterinary Advances, 2010, 5(2), 77-92.
Leishmania major in Tatera indica in Fasa, Southern Iran: Microscopy, Culture, Isoenzyme, PCR and Morphologic StudyAsian Journal of Animal and Veterinary Advances, 2011, 6(3), 255-264.
A Retrospective Analysis on Phylogeny and Evolution of CPV Isolates in ChinaAsian Journal of Animal and Veterinary Advances, 2011, 6(12), 1204-1213.
Epidemiological Studies, Seroprevalance and Some Risk Factors of Brucellosis in Sheep and Goats in the South Province of West BankAsian Journal of Animal and Veterinary Advances, 2012, 7(6), 535-539.
Powassan virus (POWV) Infection in Animals and Humans: A ReviewAsian Journal of Animal and Veterinary Advances, 2014, 9(3), 177-189.
Foamy Viruses Affecting Animals and Humans and their Public Health Concerns: A ReviewAsian Journal of Animal and Veterinary Advances, 2014, 9(2), 107-122.
Toroviruses Affecting Animals and Humans: A ReviewAsian Journal of Animal and Veterinary Advances, 2014, 9(3), 190-201.
Coronavirus Infection in Equines: A ReviewAsian Journal of Animal and Veterinary Advances, 2014, 9(3), 164-176.
Equine encephalosis virus (EEV): A ReviewAsian Journal of Animal and Veterinary Advances, 2014, 9(2), 123-133.
Insights into Bovine Tuberculosis (bTB), Various Approaches for its Diagnosis, Control and its Public Health Concerns: An UpdateAsian Journal of Animal and Veterinary Advances, 2014, 9(6), 323-344.
Capripoxviruses of Small Ruminants: Current Updates and Future PerspectivesAsian Journal of Animal and Veterinary Advances, 2016, 11(12), 757-770.
Effects of Selected Legume Species and Forage Sorghum Hay Grown in Limpopo Province on Voluntary Intake and Relative Palatability Indices of Pedi GoatsAsian Journal of Animal and Veterinary Advances, 2011, 6(12), 1249-1255.
Hematological and Serum Biochemical Parameters of Korean Native Goats Fed with Spent Mushroom SubstrateAsian Journal of Animal and Veterinary Advances, 2012, 7(11), 1139-1147.
Feed Intake, Digestibility and Growth Performance of Goats Offered Napier grass Supplemented with Molasses Protected Palm Kernel Cake and Soya WasteAsian Journal of Animal and Veterinary Advances, 2013, 8(3), 527-534.
Pathobiology of Spontaneous and Experimental Paratuberculosis (S-5 strain) in Goats with Special Reference to Early LesionsAsian Journal of Animal and Veterinary Advances, 2014, 9(8), 467-478.
Correlations of Climatic Conditions with Seminal Quality Parameters in Gaddi and Chegu Buck SemenAsian Journal of Animal and Veterinary Advances, 2020, 15(1), 32-37.
Utilization of High and Low-Quality Roughages by Sudan Desert Sheep and Nubian GoatsAsian Journal of Animal and Veterinary Advances, 2022, 17(2), 53-60.
Epidemiological Characterization of Salmonella gallinarum Isolates of Poultry Origin in India, Employing Two PCR Based Typing Methods of RAPD-PCR and PCR-RFLPAsian Journal of Animal and Veterinary Advances, 2011, 6(11), 1037-1051.
Antibiogram Assay of Salmonella Gallinarum and Other Salmonella enterica Serovars of Poultry Origin in IndiaAsian Journal of Animal and Veterinary Advances, 2012, 7(4), 309-317.
Incidence of Calf Diarrhea in Cattle and Buffalo Calves in Uttar Pradesh, IndiaAsian Journal of Animal and Veterinary Advances, 2012, 7(10), 1049-1054.
Diseases Prevalent in Equids in India: A Survey of Veterinary PractitionersAsian Journal of Animal and Veterinary Advances, 2010, 5(2), 143-153.
Seroprevalence of Peste Des Petits Ruminants in Goats in Assam, IndiaAsian Journal of Animal and Veterinary Advances, 2016, 11(3), 210-212.
Molecular Cloning and Sequence Analysis of Prion Protein Gene of Dezhou Donkey in ChinaAsian Journal of Animal and Veterinary Advances, 2006, 1(1), 23-32.
PCR-RFLP and Sequence Analysis of Hair Cytochrome b Gene for Identification of Animal SpeciesAsian Journal of Animal and Veterinary Advances, 2018, 13(2), 155-165.
Cited By
Peste des petits ruminants (PPR) in Africa and Asia: A systematic review and meta-analysis of the prevalence in sheep and goats between 1969 and 2018
Veterinary Medicine and Science, 2020, (), . DOI: 10.1002/vms3.300