Research Article 
Stressful Effect of Repeated Subcutaneous Injection of Tulathromycin on Social and Grooming Behaviors of Rats
Fatma Khalil, Naglaa M. Abdel Azeem and Abeer M. Radi
Asian Journal of Animal and Veterinary Advances, 2016, 11(3), 178-183.
Abstract
Stressful effect of repeated subcutaneous injection of tulathromycin on serum cortisol level and behavior of rats was investigated. Twenty seven apparently healthy adult male rats were equally divided into three groups. Group 1 was subcutaneously injected with tulathromycin in a dose of (2.5 mg kg–1 b.wt.) twice with 15 days interval, group 2 was injected for three successive injections with one week interval and control group rats were subcutaneously injected with 0.1 mL normal saline once weekly for three weeks. Rat’s behavior was videotaped. Thereafter, frequency and duration/min of rats behaviors (Self-grooming, feeding, comfort and locomotion) were calculated and their aggression bouts were scored. At the end of the experiment, blood samples were collected and serum was obtained for measuring liver and kidney enzymes and serum cortisol level. Cortisol level was increased to 1.4 and 1.5 times that of control in group 1 and group 2, respectively, while the enzymes activities were not significantly altered after the end of the experiment. At the second week, group 2 rats performed self-grooming for longer time than control rats. However, at the third week of injection, self-grooming duration of rats in both groups was shorter than those in control. Furthermore, group1 rats showed marked social behavior disturbance expressed in prominent aggression in between. Data suggested stressful effect of repeated subcutaneous injection of tulathromycin to rats. Thus, repeated subcutaneous injection of tulathromycin is not recommended for animals.
ASCI-ID: 13-819
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