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Research Article
Effect of Pineapple Extracts on the Reproduction of Rhipicephalus sanguineus Semi-Engorged Females

Amornrat Juasook, Thidarut Boonmars, Atchara Artchayasawat, Benjamabhorn Pumhirunroj, Sirintip Boonjaraspinyo, Pranee Sriraj and Ratchadawan Aukkanimart

Asian Journal of Animal and Veterinary Advances, 2018, 13(4), 339-345.

Abstract

Background and Objective: Rhipicephalus sanguineus (R. sanguineus), known as the brown dog tick, is a main vector of several important pathogens in dog and occasionally in humans. The present study investigated the in vitro effects of pineapple skin and core extracts on Rhipicephalus sanguineus semi-engorged females compared with a commercial chemical insecticide, in a search for new alternative methods to control this ectoparasite. Materials and Methods: A total of 180 female ticks with homogeneous weight were divided into four groups with three replications and each group was immersed for 5 min in the following solutions: 0.25% w/v fipronil (FIP), pineapple skin (PAS) extract, pineapple core (PAC) extract, or distilled water (CON) for the control group. After the immersion, the ticks were incubated at 28°C, RH>80% and a 12 h photoperiod for 14 days to analyze the toxicity and effects on morphological changes, mortality rates, laying rates and oocyte histological appearances. Data were analyzed using analysis of variance (one-way ANOVA). Results: The laying rates of female ticks in the treated groups (FIP 0%, PAC 26.70% and PAS 20.00%) were significantly lower than in the control group (46.70%) (p<0.05). The mortality rates in treated groups (PAC 100%, PAS and FIP 93.33%) were higher than in the control group (46.67%). For oocyte histology result, pineapple extracts had effect on structural changes in oocyte development which was similar to the effect of the chemical compound, including large vacuolated cytoplasm, size variations, laceration and withering of yolk granules. Conclusion: Extracts from the skin and core of pineapple can be considered as potent natural agents that can reduce the reproduction of R. sanguineus females and control tick population. However, the active ingredients and mechanism of this plant should be further clarified to improve the acaricidal efficiency.

ASCI-ID: 13-989

Cited References Fulltext

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