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Research Article
Study on Apparent Amylose Content in Context of Polymorphism Information Content along with Indices of Genetic Relationship Derived through SSR Markers in Birain, Bora and Chokuwa Groups of Traditional Glutinous Rice (Oryza sativa L.) of Assam

B. Shaptadvipa and R.N. Sarma

Asian Journal of Biochemistry, 2009, 4(2), 45-54.


Amylose content was determined in 41 traditional glutinous rice varieties of Assam classed as Birain, Bora and Chokuwa group during 2004-06. Average apparent amylose content in 6 accessions of Chokuwa (9.368%) was higher than 20 accessions of Bora group (0.502%) and 15 accessions of Birain (0.191%) genotypes. Mahsuri, a non-glutinous rice variety contained intermediate amylose content (21.2%). Eight SSR markers were used to assess genetic variability. The size of amplified fragments ranged from 100 to 500 bp. Among all genotypes, average Polymorphism Information Content (PIC) was 0.923. The average genetic similarity within the Birain accessions ranged from 0.119 to 0.571. Within Bora-Chokuwa accessions, similarity value ranged from 0.047 to 0.667. The average similarity was 0.228, which reflected that the Bora group could be more diverse than the Birain group. Amylose content is said to be highly influenced by environmental conditions. Since, Birain accessions were from the same Barak valley agro-climatic condition and Bora as well as Chokuwa were from the Brahmaputra valley, an analysis was made with corresponding pair-wise relative rate of increase (%) in apparent amylose contents as well as corresponding values of pair-wise Jaccard’s co-efficient of similarity among the accessions of Birain, Bora and Chokuwa groups of glutinous rice. It showed the existence of a matching relation between the increased values of respective apparent amylose content and the genetic similarity. It seems that apparent amylose content though cannot play a solid indicator for genetic variability in glutinous rice germplasm. However it may help to gauge biochemical bases towards genetic variability under same environmental condition.

ASCI-ID: 16-165

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