Search. Read. Cite.

Easy to search. Easy to read. Easy to cite with credible sources.

Research Article
Oxidative Stress Indicators of Human Sickle Erythrocytes Incubated in Aqueous Extracts of Three Medicinal Plants

P.C. Chikezie

Asian Journal of Biochemistry, 2011, 6(5), 373-383.

Abstract

In vitro study was carried out to investigate levels of oxidative stress indicators of sickle erythrocytes incubated in aqueous extracts of Anacardium occidentale, Psidium guajava and Terminalia catappa for 12 h. At regular time intervals of 3 h, portions of the incubation mixtures were withdrawn and spectrophotometric method was used to assay for levels of erythrocyte Malondialdehyde (MDA) and methaemoglobin (Met. Hb%). The control analysis showed that within the experimental time, erythrocyte MDA increased from 2.45±0.35 to 3.13±0.59 mmol mL-1 (p>0.05; pvalue = 0.801176). Erythrocyte MDA concentrations in the presence of the three extracts were higher than the control samples at t = 3 h (p>0.05; p value = 0.963253). Compared with the control samples at the given time (t) intervals, extract of T. catappa exhibited the highest capacity to cause reduction of erythrocyte MDA ([T. catappa] = 800 mg%; [MDA] = 2.89±0.33 mmol mL-1; t = 12 h). Erythrocyte Met. Hb% increased from 2.42±0.55 to 2.51±0.49% (p>0.05; p value = 0.995171) in the control samples within 12 h. Incubation of sickle erythrocytes with extract of (P. guajava) = 800 mg% for 9 h caused reduction of Met. Hb% from 2.49±0.49 to 2.29±0.45%; p>0.05; p = 0.983519. Extracts of A. occidentale, P. guajava and T. catappa exhibited variable capacities to hinder lipid peroxidation but did not cause corresponding reduction in erythrocyte Met. Hb%, exemplified by negative correlation between the two oxidative stress indicators in the presence of T. catappa and higher concentrations of A. occidentale and P. guajava.

ASCI-ID: 16-251

Cited References Fulltext

Related Articles


Methaemoglobin Content and NADH-methaemoglobin Reductase Activity of Three Human Erythrocyte Genotypes

Asian Journal of Biochemistry, 2011, 6(1), 98-103.

Comparative Erythrocyte Glutathione S-Transferase Activity Profile of Non-Malarious Guinea Pigs (Cavia tschudii) Administered with Pyrimethamine/Sulphadoxine and Artemether/Lumefantrine Combination Therapies

Asian Journal of Biochemistry, 2014, 9(4), 203-212.

L-Arginine Ameliorates Arylesterase/Paraoxonase Activity of Paraoxonase-1 in Hypercholesterolemic Rats

Asian Journal of Biochemistry, 2011, 6(3), 263-272.

Protective Effect of Tecoma stans Flowers on Gentamicin-Induced Nephrotoxocity in Rats

Asian Journal of Biochemistry, 2016, 11(1), 59-67.

Immunohistochemical Expression of Insulin and Glucagon, Superoxide Dismutase and Catalase Activity in Pancreas in Hyperglycaemia Condition

Asian Journal of Biochemistry, 2016, 11(4), 177-185.

Pomegranate (Punica granatum L.) Juice Improves Liver Damage in Carbon Tetrachloride-induced Rats

Asian Journal of Biochemistry, 2017, 12(3), 79-84.

Pumpkin and Vitamin E as Potent Modulators of Apoptosis in Gentamicin-induced Rat Nephrotoxicity

Asian Journal of Biochemistry, 2018, 13(1), 1-8.

Methaemoglobin Content and NADH-methaemoglobin Reductase Activity of Three Human Erythrocyte Genotypes

Asian Journal of Biochemistry, 2011, 6(1), 98-103.

Cited By


Protective Effect of Silymarin on Human Erythrocyte Against Tert-butyl Hydroperoxide Induced Oxidative Stress in vitro

American Journal of Biochemistry and Molecular Biology, 2012, 2(3), 167. DOI: 10.3923/ajbmb.2012.167.174