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Research Article
Comparative in vitro Osmotic Stability of Three Human Erythrocyte Genotypes in the Presence of Quinine and Chloroquine Phosphate

P.C. Chikezie

Asian Journal of Biochemistry, 2011, 6(1), 55-64.

Abstract

The aim of the present in vitro study was to ascertain the tendency of two quinoline (quinine and chloroquine phosphate) drugs to interfere with osmotic stability of three human erythrocyte genotypes, namely, HbAA, HbAS and HbSS. Spectrophotometric method was used for determination of the capacity of the erythrocyte genotypes to withstand osmotic stress in the presence of separate increasing concentrations (0.2, 0.4, 0.6 and 0.8 mg%) of the two antimalarials. The Mean Corpuscular Fragility (MCF) index of the three genotypes was in the order: HbAA<HbAS<HbSS irrespective of the malaria status of the blood donors. Whereas there was no significant difference (p>0.05) between the MCF values of non-malarious blood samples of HbAA and HbAS erythrocytes, values between HbAA and HbSS erythrocytes showed significant difference (p<0.05). In addition, parasitized erythrocytes exhibited significant (p<0.05) increased MCF values. Furthermore, at relative low experimental concentrations (approx<0.4 mg %) of the two drugs, parasitized erythrocytes and those of non-malarious human origin of HbAA and HbAS genotypes showed variable levels of stability. The HbSS erythrocytes did not exhibit osmotic stability within the range of experimental concentrations of the two drugs. The implications of these findings are discussed.

ASCI-ID: 16-214

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