Research Article
Biological Screening of Some Sewage Microbes in Bangladesh

M.E. Haque, M.M. Pervin, M.Z. Rahman, K.M.K.B. Ferdaus, M.H. Kabir, M.A. Rahman, M.A. Bari Miah, M.R. Islam and M.T. Hoque

Asian Journal of Plant Sciences, 2005, 4(6), 625-629.

Abstract

The aim of present study was to investigate the bioactivity (antibacterial, antifungal and cytotoxicity etc.) of the ethylacetate extract isolated from Streptomyces sp. Aspergillus sp. and Bacillus sp. from sewage of different regions of Bangladesh. The ethylacetate extract of Streptomyces sp., Aspergillus sp. and Bacillus sp. shown modest antibacterial and antifungal activities at the concentration of 200 μg disc-1. The maximum antibacterial and antifungal activities were shown by the Streptomyces sp. The minimum inhibitory concentration of the microbial extracts was between 64 to 128 μg mL-1 against test organisms. Brine srimp lethality bioassay was carried out for cytotoxicity measurement of the extracts and the LC50 values were calculated after probit transformation of the resulting mortality data. All the ethyl acetate extract showed lower cytotoxicity properties (Streptomyces sp. 42.37 μg mL-1, Aspergillus sp. 52.25 μg mL-1 and Bacillus sp. 47.51 μg mL-1) compared with the reference standard Bleomycin (0.41 μg mL-1) and Galic acid (4.53 μg mL-1).

ASCI-ID: 7-846

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