Research Article 
Control of Mycoflora of Farmer`s Stored Seeds of Mungbean
Joyjit Barua, M. Mahboob Hossain, Ismail Hossain, A.A.M. Syedur Rahman and Md. Abu Taher Sahel
Asian Journal of Plant Sciences, 2007, 6(1), 115-121.
Abstract
The prevalence of fungi and bacteria associated with mungbean (Vigna radiata) seeds and their control were studied during the month of January-November, 2004. Twenty seed samples of mungbean were collected from two villages of Mymensingh district. The germination percentage of all samples of mungbean remarkably varied. Hot water treatment at 53°C for 15 min showed comparatively better performance than Vitavax-200 at the rate of 2.5 g kg-1 in case of germination. Seed-borne fungi were recorded and detected by using blotter method. Four different fungal pathogens were identified, viz. Aspergillus flavus, Aspergillus niger, Fusarium spp. (F. oxysporum, F. moniliforme, F. semitectum) and Penicillium spp. Two groups of bacteria were identified on un-germinated seeds and emerging radicles. Yellow and creamy colored bacterial colonies were found in seed surface during blotter incubation test. It was also observed that liquid assay was better than the washing test and blotter method for detecting pathogenic bacteria. Xanthomonas campestris pv. vignicola and Pseudomonas syringae pv. syringae were identified. Efficacy of two seed treating methods viz. Vitavax-200 and hot water were tested in the laboratory. Mungbean seeds treated with Vitavax-200 at the rate of 2.5 g kg-1 showed highest control of seed-borne fungi than hot water treatment. Hot water treatment at 55°C temperature for 15 min also effective to reduction of seed-borne fungi and bacterial infections. But at 55°C of hot water treatment hampered the germination percentage of seeds. Hot water treatment at 53°C temperature for 15 min was effective for controlling seed-borne mycoflora next to Vitavax-200at the rate of 2.5 g kg-1.
ASCI-ID: 7-1073
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