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Research Article
Effect of NaCl on Growth and Development of in vitro Plants of Date Palm (Phoenix dactylifera L.) ‘Khainazi’ Cultivar

Saleh Alturki

Asian Journal of Plant Sciences, 2018, 17(3), 120-128.

Abstract

Background and Objectives: High salinity of soil and water resources is a global issue which is causing substantial decline of productive lands in many arid and semiarid regions of the world due to salinization. Therefore, the main objective of this study was to determine the effect of NaCl on growth and development of in vitro plants of date palm (Phoenix dactylifera L.) ‘Khainazi’ cultivar. Materials and Methods: The study was carried out in the Tissue Culture Laboratory, Date Palm Research Center, King Faisal University, Kingdom of Saudi Arabia. Ten date palm offshoots of cv. Khainazi, about 3-4 years old and weighing 5-7 kg were used in the experiment. The shoot tip and lateral buds were sterilized in 20% v/v clorex solution for 15 min and then sectioned into 1 cm explants for culture initiation. The modified MS media was supplemented with different concentrations of NaCl ranging from 0, 50, 100, 200, 300 and 400 mmol L–1 and incubated at 25±2°C for 16 h in light daily supplied by 65/80 Warm White Weisse 3500 fluorescent tubes. Each treatment was replicated 10 times by following a completely randomized design. Plant observations included dry weight of shoots and roots and length of stem and roots. The concentration of Na, Mg, Ca and K in plant roots and shoots were determined by the Atomic Absorption Spectrophotometer while Cl by a Chloride meter. Experimental data were analyzed by following appropriate statistical procedures. Results: Stem and root length and dry weight were progressively reduced in most of the treatments by the addition of 0, 50, 100, 200, 300 and 400 mmol L–1 to the growth medium. The reduction in shoot, however, was greater than root. The concentration of Na increased significantly both in the shoots and roots. While the concentration of Ca, Mg and K increased with the addition of NaCl up to 100 mmol L–1, then decreased with increasing the NaCl concentration up to 400 mmol L–1 in the growth medium.. Conclusion: Addition of high NaCl concentration to growth medium for the development of date palm off-shoots using tissue culture technology did not show encouraging results. In conclusion, addition of NaCl solution having concentration above 100 mmol L–1 to growth medium proved detrimental to explants to obtain desired date palm off shoots.

ASCI-ID: 7-1773

Cited References Fulltext

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