Research Article 
Effect of 6-Benzyladenine Purine, α-Naphthalene Acetic Acid, Coconut Water and Potato Extract on Micropropagation of Ensete glaucum from Vietnam
Xuan Duong Vu, Thi Tam Tien Ha, Thanh Loan Pham, Luong Thi Hien Dao and Phi Bang Cao
Asian Journal of Plant Sciences, 2023, 22(2), 374-381.
Abstract
Background and Objective: Ensete glaucum (Roxb.) Cheesman is widely dispersed and has ornamental value as well as potential medicinal benefits. This research aimed to discover the effect of BAP, NAA and two organic additives, coconut water and potato extract on shooting and rooting during in vitro propagation of Ensete glaucum collected from Xuan Son Nation Park, Phu Tho Province, Vietnam. Materials and Methods: For shoot production, sterilized shoot tip explants of E. glaucum (1 cm in height) were placed in cultured media containing BAP at different concentrations (0, 1.0, 2.0, 3.0 and 4.0 mg L–1) combined with 0.2 mg L–1 NAA for the first experiment. Coconut water (CW) (0, 50, 100, 150 and 200 mL L–1) and potato extract (PE) (0, 10, 20, 30 and 40 g L–1) combined with BAP at the optimum concentration obtained from the previous experiment was used for the second experiment. For rooting, explants of E. glaucum (3 cm in height) were transferred in cultured media containing different concentrations of NAA (0, 0.5, 1.0, 1.5 and 2.0 mg L–1) combined with 0.2 mg L–1 BAP for the first experiment. In the second experiment, explants of E. glaucum were cultured in the medium added CW (0, 50, 100, 150 and 200 mL L–1) and PE (0, 10, 20, 30 and 40 g L–1) combined with NAA at the optimum concentration obtained from the previous experiment to investigate the effect of these organic additives. Obtained data were statistically analyzed by using Duncan’s Multiple Range Test (p = 0.05). Results: The BAP at the concentration of 3 mg L–1 combined with 0.2 mg L–1 NAA gave the highest mean number of shoots produced per explant and shoot length while, 2 mg L–1 BAP combined with 0.2 mg L–1 NAA acquired the highest mean number of leaves. The NAA at 0.5 mg L–1 (combined with BAP at 0.2 mg L–1) was the most favorable for the rooting of E. glaucum. Medium supplemented with CW (100 mL L–1) or PE (30 g L–1 for shooting and 20 g L–1 for rooting, respectively) resulted in an increase in shoot multiplication and root generation during micropropagation of E. glaucum. Conclusion: The present study first represents an investigation of the effect of BAP, NAA, coconut water and potato extract on micropropagation of E. glaucum collected in Vietnam.
ASCI-ID: 7-2083
Similar Articles
Development of a Highly Efficient Callus Induction and Regeneration Protocol for Sugarcane using Apical Meristem
Asian Journal of Plant Sciences, 2022, 21(1), 78-87.
Effect of Duration of Light:Dark Cycles on in vitro Shoot Regeneration of TomatoAsian Journal of Plant Sciences, 2005, 4(3), 255-260.
Micropropagation of Water Chestnut (Trapa sp.) Through Local Varieties of Rajshahi DivisionAsian Journal of Plant Sciences, 2006, 5(3), 409-413.
Brassinosteroids Influences in vitro Regeneration Using Shoot Tip Sections of Cymbidium elegans LindlAsian Journal of Plant Sciences, 2007, 6(2), 308-313.
An Improved System for in vitro Propagation of Banana (Musa acuminate L.) CultivarsAsian Journal of Plant Sciences, 2008, 7(1), 116-118.
Seed Germination and in vitro Propagation of Agave grijalvensis an Endemic Endangered Mexican SpeciesAsian Journal of Plant Sciences, 2008, 7(8), 752-756.
Genetic Stability in Date Palm MicropropagationAsian Journal of Plant Sciences, 2008, 7(8), 775-778.
Effect of Explants Density on the in vitro Proliferation and Growth of Separated and Cluster Shoots of Smooth Cayenne Pineapple (Ananas comosus L. Merr.)Asian Journal of Plant Sciences, 2009, 8(4), 313-317.
Nano-Silver Particles Eliminate the in vitro Contaminations of Olive ‘Mission’ ExplantsAsian Journal of Plant Sciences, 2009, 8(7), 505-509.
Factors Affecting the in vitro Multiplication of the Endemic Zingiber Curcuma haritha Mangaly and SabuAsian Journal of Plant Sciences, 2006, 5(5), 847-853.
Micro-propagation and 1 ’, 2’-didehydrostemofoline Production from Stemona Sp.Asian Journal of Plant Sciences, 2011, 10(6), 338-341.
The Effect of Cytokinin Type and Concentration on Multiplication Rate of Spathiphyllum (Fam. Araceae)Asian Journal of Plant Sciences, 2005, 4(4), 401-404.
In vitro Micropropagation of Ere Meraat (Aloe percrassa Tod.) Using Explants from OffsetsAsian Journal of Plant Sciences, 2014, 13(2), 66-72.
Morphological and Genetic Variations of Micropropagated Paulownia Hybrid (P. elongata X P. fortunei) Using Gamma IrradiationAsian Journal of Plant Sciences, 2021, 20(3), 396-405.
Antioxidant Activity, Active Compounds and Oil Characterization of in vitro Sequoia sempervirens CulturesAsian Journal of Plant Sciences, 2021, 20(4), 673-683.
Relation Between Culture Medium, Hormones Combination and Shoots Proliferation of Plum Cv. “Santa Rosa”Asian Journal of Plant Sciences, 2021, 20(1), 157-162.
Effects of Copper Sulphate on Shoot Multiplication and Rooting of Banana (Musa acuminata L.) (In vitro Study)Asian Journal of Plant Sciences, 2020, 19(3), 200-204.
Validating the Efficacy of an Established Micropropagation Protocol for Commercial Propagation of Neolamarckia cadambaAsian Journal of Plant Sciences, 2023, 22(3), 485-495.
Evaluation of Cotyledonary Node of Clitoria ternatea L. for High Frequency in vitro Axillary Shoot ProliferationAsian Journal of Plant Sciences, 2010, 9(6), 351-357.
Cited By
Vi nhân giống chuối hoa sen (Ensete Glaucum (Roxb.) Cheesman)
CTU Journal of Science, 2026, 62(3), 233. DOI: 10.22144/ctujos.2026.129