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Research Article
In vitro Shoot Tip Culture of Sugar-cane (Saccharum officinarum) Variety Isd 28

R. Baksha, R. Alam, M.Z. Karim, S.K. Paul, M.A. Hossain, M.A.S. Miah and A.B.M.M. Rahman

Biotechnology, 2002, 1(2), 67-72.

Abstract

Multiple shoots were obtained from shoot tip explant of sugar-cane (Saccharum officinarum) cultured on MS medium supplemented with BAP (0.5-2.0 mg l-1), Kn (0.1-0.5 mg l-1) and IBA (0.1-0.5 mg l-1). Roots were induced in in vitro regenerated shoots on half MS medium supplemented with 5.0 mg l-1 NAA, IBA and IAA. Plant regeneration from shoot tip was the highest on MS medium supplemented with BAP 2.0 mg l-1 and IBA 0.5 mg l-1. The reported experimental findings present a method of plant regeneration of sugar-cane variety Isd 28 through shoot tip culture. The plantlets were successfully transferred to soil and the percentage of survivability under ex vitro condition was 70.

ASCI-ID: 11-40

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Journal of Biological Sciences, 2003, 3(10), 915. DOI: 10.3923/jbs.2003.915.920