In vitro Shoot Multiplication of Chrysanthemum morifolium as Affected by Sucrose, Agar and pH
2003, 2(2), 115-120.
Effects of sucrose, agar and pH on in vitro shoot multiplication
of Chrysanthemum were studied. Nodal explant from the ex vitro grown
plant was used as the test material. For optimum shoot induction and multiplication
in MS medium containing BAP+ sucrose 30 gm l-1, agar 6 gm l-1
and pH 5.5-6.0 proved more effective. The media having 30 gm l-1
sucrose showed the highest percentage of explant responded to shoot proliferation
and that was 100%. This sucrose concentration also showed the optimum result
for number of usable shoots per culture, number of node shoot-1 and average
length of shoots and the values were 5.4±0.6, 5.1±0.8 and 5.6±0.4
cm. The highest proliferation response of the explant was observed on MS medium
having 6 gm l-1 of agar and the frequency was 100%. Among different
level of pH, the highest percentage of explant showing proliferation was observed
on the media adjusted to pH 5.5 and 6.0. The results presented here proved to
be suitable for the in vitro shoot multiplication of Chrysanthemum
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