Search. Read. Cite.

Easy to search. Easy to read. Easy to cite with credible sources.

Research Article
Random Amplification of Polymorphic DNA (RAPD) of Salmonella enteritidis Isolated from Chicken Samples

A. Maripandi, Suresh S.S. Raja, P. Ponmurugan and G. Gurusubramanian

Biotechnology, 2007, 6(2), 278-282.

Abstract

An attempt was made to study RAPD using 30 isolates of S. enteritidis obtained from chicken samples for the purpose of strain discrimination. The primer used was P1254, (5’CCGCAGCCAA3’) to amplify the genomic fragment of S. enteritidis isolates, which resulted in production of six clones. Six isolates each in clone 1, 2 and 5 and another four isolates each in clone 2, 4 and 6, respectively. In clone 1, having an additional 900 bp amplified products but it was lacking in clones 2 and shared a common 500 bp fragments. Clone 3 had additional amplified fragment at 450 bp. It was lacking in clone 4 and clone 5 had the additional amplified products at 950, 900, 750 and 560 bp but it was lacking in clone 6. The final cluster 6 had three fragments with molecular weight 1550, 1300 and 400 bp, respectively. To sum up, there were 6 distinct clones of S. enteritidis serovars identified based on RAPD profiling.

ASCI-ID: 11-279

Cited References Fulltext

Similar Articles


Investigation on Intera-Specific Biodiversity of 51 Peanut Cocoon Strains of Iran Silkworm (Bombyx mori) Germplasm Based on Reproductive Traits

Biotechnology, 2010, 9(2), 149-156.

Molecular Markers Associated with Resistance to Schistosoma mansoni Infection in the Biomphalaria glabrata Snails

Biotechnology, 2006, 5(4), 404-412.

Molecular Typing of Iranian Cladosporium Isolates Using RAPD-PCR

Biotechnology, 2008, 7(4), 763-768.

Characterization of Staphylococcus aureus Isolated from the Skin Surface of Athletes and Training Environment by Random Amplified Polymorphic DNA and Antibiotic Resistance Profiling

Biotechnology, 2006, 5(4), 489-494.

Genetic Diversity in Bean Populations Based on Random Amplified Polymorphic DNA Markers

Biotechnology, 2008, 7(1), 1-9.

Cited By


Application of an optimized 18-h method involving one step culturing and single primer-based PCR assay for detection of Salmonella spp. in foods

Food Control, 2010, 21(5), 593. DOI: 10.1016/j.foodcont.2009.08.012

Epidemiological Characterization of Salmonella gallinarum Isolates of Poultry Origin in India, Employing Two PCR Based Typing Methods of RAPD-PCR and PCR-RFLP

Asian Journal of Animal and Veterinary Advances, 2011, 6(11), 1037. DOI: 10.3923/ajava.2011.1037.1051

Molecular Characterization of Salmonella enterica Serovars Isolated from a Turkey Production Facility in the Absence of Selective Antimicrobial Pressure

Foodborne Pathogens and Disease, 2016, 13(2), 80. DOI: 10.1089/fpd.2015.2002

An Optimized EMA-RAPD-PCR for a Reliable Detection of Viable Salmonella spp. in Chicken Products

Journal of Food Safety, 2013, 33(3), 247. DOI: 10.1111/jfs.12046

Salmonella enterica isolates from pasture-raised poultry exhibit antimicrobial resistance and class I integrons

Journal of Applied Microbiology, 2010, 109(6), 1957. DOI: 10.1111/j.1365-2672.2010.04825.x