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Research Article
Random Amplification of Polymorphic DNA (RAPD) of Salmonella enteritidis Isolated from Chicken Samples

A. Maripandi, Suresh S.S. Raja, P. Ponmurugan and G. Gurusubramanian

Biotechnology, 2007, 6(2), 278-282.


An attempt was made to study RAPD using 30 isolates of S. enteritidis obtained from chicken samples for the purpose of strain discrimination. The primer used was P1254, (5’CCGCAGCCAA3’) to amplify the genomic fragment of S. enteritidis isolates, which resulted in production of six clones. Six isolates each in clone 1, 2 and 5 and another four isolates each in clone 2, 4 and 6, respectively. In clone 1, having an additional 900 bp amplified products but it was lacking in clones 2 and shared a common 500 bp fragments. Clone 3 had additional amplified fragment at 450 bp. It was lacking in clone 4 and clone 5 had the additional amplified products at 950, 900, 750 and 560 bp but it was lacking in clone 6. The final cluster 6 had three fragments with molecular weight 1550, 1300 and 400 bp, respectively. To sum up, there were 6 distinct clones of S. enteritidis serovars identified based on RAPD profiling.

ASCI-ID: 11-279

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