Random Amplification of Polymorphic DNA (RAPD) of Salmonella enteritidis Isolated from Chicken Samples
Suresh S.S. Raja,
2007, 6(2), 278-282.
An attempt was made to study RAPD using 30 isolates of S. enteritidis obtained from chicken samples for the purpose of strain discrimination. The primer used was P1254, (5CCGCAGCCAA3) to amplify the genomic fragment of S. enteritidis isolates, which resulted in production of six clones. Six isolates each in clone 1, 2 and 5 and another four isolates each in clone 2, 4 and 6, respectively. In clone 1, having an additional 900 bp amplified products but it was lacking in clones 2 and shared a common 500 bp fragments. Clone 3 had additional amplified fragment at 450 bp. It was lacking in clone 4 and clone 5 had the additional amplified products at 950, 900, 750 and 560 bp but it was lacking in clone 6. The final cluster 6 had three fragments with molecular weight 1550, 1300 and 400 bp, respectively. To sum up, there were 6 distinct clones of S. enteritidis serovars identified based on RAPD profiling.
Cited References Fulltext
Investigation on Intera-Specific Biodiversity of 51 Peanut Cocoon Strains of Iran Silkworm (Bombyx mori) Germplasm Based on Reproductive Traits
2010, 9(2), 149-156.
Molecular Markers Associated with Resistance to Schistosoma mansoni Infection in the Biomphalaria glabrata Snails
2006, 5(4), 404-412.
Molecular Typing of Iranian Cladosporium Isolates Using RAPD-PCR
2008, 7(4), 763-768.
Characterization of Staphylococcus aureus Isolated from the Skin Surface of Athletes and Training Environment by Random Amplified Polymorphic DNA and Antibiotic Resistance Profiling
2006, 5(4), 489-494.
Genetic Diversity in Bean Populations Based on Random Amplified Polymorphic DNA Markers
2008, 7(1), 1-9.
Application of an optimized 18-h method involving one step culturing and single primer-based PCR assay for detection of Salmonella spp. in foods
2010, 21(5), 593.
Epidemiological Characterization of Salmonella gallinarum Isolates of Poultry Origin in India, Employing Two PCR Based Typing Methods of RAPD-PCR and PCR-RFLP
Asian Journal of Animal and Veterinary Advances,
2011, 6(11), 1037.
Molecular Characterization of Salmonella enterica Serovars
Isolated from a Turkey Production Facility in the Absence of Selective
Foodborne Pathogens and Disease,
2016, 13(2), 80.
An Optimized EMA-RAPD-PCR for a Reliable Detection of Viable Salmonella
spp. in Chicken Products
Journal of Food Safety,
2013, 33(3), 247.
Salmonella enterica isolates from pasture-raised poultry exhibit antimicrobial resistance and class I integrons
Journal of Applied Microbiology,
2010, 109(6), 1957.