Research Article 
Gene Expression Profile of Synovial Cells in Experimental Post-Traumatic Arthritis of Knee in Swine
O. Baatartsogt, K. Choi, P.K. Mandal, Hee-kyong Lim, Guan-Hao Li, Hong-Sik Kong, Dae-Hyun Hahm, Chi-Ho Lee and Jun-Heon Lee
Biotechnology, 2009, 8(1), 70-77.
Abstract
To get insights into pathological pathways of Post Traumatic Arthritis (PTA) this study was done on gene expression profile of synovial cells of knee using gene chip analysis developing experimental post traumatic arthritis (ETA) in swine. ETA was induced by transection of the Anterior Cruciate Ligament (ACL) of left knee in 3 piglets. Articular cartilage and synovial tissues were obtained after 0, 2, 5 and 8 weeks for histopathologic examination. Synovial cells collected after sacrificing the piglet at 8 weeks, were used at 5 passage for gene expression profiling using Affimetrix Gene Chip. Histopathologic examination showed overt chronic inflammation indicating the development of ETA. Through genome analyses it was observed that 87 known genes were up-regulated and 76 known genes were down regulated. By analyzing, it was found that many genes with differential expression are related to inflammation, immune response, lipid binding, cell adhesion, growth activity and muscle development. The present study provided an insight into the TA related gene expression pattern. The genome analysis of synovial cells provided us new candidate molecules which may be useful to understand the pathogenesis of Post Traumatic Arthritis (PTA). The established porcine model may serve as in vivo disease model for further research on traumatic arthritis to elucidate molecular pathogenesis.
ASCI-ID: 11-457