Research Article
In vitro Seed Germination and Micropropagation of Edible Bamboo Dendrocalamus giganteus Munro using Seeds

W. Sayanika Devi, L. Bengyella and G.J. Sharma

Biotechnology, 2012, 11(2), 74-80.

Abstract

Successful propagation from seeds of Dendrocalamus giganteus Munro was achieved by in vitro methods. Incidence of sporadic flowering was recorded. The descriptions of inflorescence and floral morphology are in agreement with the prior taxonomic descriptions but a more detailed description and illustrations are presented in this communication. In vitro seed germination was enhanced in gibberellic acid (GA3) supplemented liquid Murashige and Skoog (MS) medium in the presence of light. Shoots multiplication was obtained directly from seeds inoculated on MS medium containing benzylaminopurine (BAP). In another method, callus was induced and proliferated on MS medium supplemented with 3 mg L-1 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5 mg L-1 kinetin (Kn). De novo production of shoots took place from white, compact and nodular calli after transfer to MS medium containing 1 mg L-1 naphthalene acetic acid (NAA) and 0.5 mg L-1 Kn. Regenerated shoots were excised and induced to root on media with auxin. In vitro rooting percentage of shoots reached 86% with half-strength MS medium containing 5 mg L-1 indole-3-butyric acid (IBA). After the process of hardening and acclimatization, the plantlets established well when transferred to field during favourable season.

ASCI-ID: 11-675

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