Research Article 
Effect of Explant Type in Development of in vitro Micropropagation Protocol of an Endangered Medicinal Plant: Curcuma caesia Roxb.
Neha Behar, K.L. Tiwari and S.K. Jadhav
Biotechnology, 2014, 13(1), 22-27.
Abstract
Curcuma caesia Roxb. belonging to family Zingiberaceae is an important traditional medicinal plant. The rhizome has been widely used as folklore medicine. Chhattisgarh state medicinal plant board has categorized it as an endangered plant. Turmeric/ginger is vegetatively propagated exclusively through underground rhizomes and multiplication rate is very low. Furthermore, susceptibility to diseases, specifically soft rot caused by Pythium species causes heavy losses. In vitro micropropagation technique can be useful for conservation and commercial exploitation of valuable secondary metabolites in medicinal plants. In vitro plant regeneration system depends upon many factors but the type of explants has been identified as one of the major factor. Thus, the present study was conducted using various parts of the plant viz. leaf, root, rhizome sections, mature bud of rhizome and sprouted bud of rhizome in different hormone concentration and combinations in MS medium. Only mature bud and sprouted bud from the rhizome responded while others did not show any sign of morphogenesis. Sprouted and mature bud showed best response in 4 mg L-1 BAP+100 mg L-1 ADS with mean 3.8±0.32 number of shoots and mean 3.28±0.42 cm length, similarly, 1±0.39 mean shoots and mean 1.17±0.48 cm length in mature bud. In vitro produced plants were easily established in soil with almost 100% survival and were morphologically similar to their parent plants. Thus, sprouted buds of the rhizome can be exploited for further micropropagation and conservation studies.
ASCI-ID: 11-747
Similar Articles
Influence of Pre-Sowing Treatments on in vitro Seed Germination of Ativisha (Aconitum heterophyllum Wall) of Uttarakhand
Biotechnology, 2011, 10(2), 215-219.
Development of Alfalfa Tolerant to Salinity Stress Using Organogenesis TechniqueBiotechnology, 2004, 3(2), 136-139.
Effect of Medium pH on Shoot Regeneration from the Cotyledonary Explants of TomatoBiotechnology, 2005, 4(1), 7-10.
In Vitro Micropropagation of (Vicia faba L.) Cultivars ‘Waza Soramame and Cairo 241’ by Nodal Explants Proliferation and Somatic EmbryogenesisBiotechnology, 2006, 5(1), 32-37.
Effect of Growth Regulators and Carbon Sources on Axillary Shoot Proliferation from Shoot-Tip Explant and Successful Transplantation of Papaya (Carica papaya L.)Biotechnology, 2007, 6(2), 268-272.
Tissue Culture and RAPD Analysis of Cinnamomum camphora and Cinnamomum verumBiotechnology, 2007, 6(2), 239-244.
In vitro Shoot Initiation from Nodal Explants of Jojoba (Simmondsia chinensis) StrainsBiotechnology, 2007, 6(2), 165-174.
In vitro Shoot Multiplication of Six Promising Strains of Jojoba (Simmondsia chinensis)Biotechnology, 2007, 6(3), 309-315.
In vitro Root Formation in Micropropagated Shoots of Jojoba (Simmondsia chinensis)Biotechnology, 2007, 6(4), 465-472.
Foliar Regeneration in Anthurium andraeanum Hort. cv. AgnihothriBiotechnology, 2008, 7(1), 134-138.
In vitro Shoot Cut: A High Frequency Multiplication and Rooting Method in the Bamboo Dendrocalamus hamiltoniiBiotechnology, 2009, 8(2), 259-263.
In Vitro Micropropagation of Yew (Taxus Baccata) and Production of PlantletsBiotechnology, 2010, 9(1), 48-54.
Determination of Genetic Integrity in Long-term Micropropagated Plantlets of Allium ampeloprasum L. Using ISSR MarkersBiotechnology, 2010, 9(2), 218-223.
A Novel Strategy for in vitro Conservation of Aloe vera L. through Long Term Shoot CultureBiotechnology, 2010, 9(3), 326-331.
Somatic Embryogenesis of Date Palm (Phoenix dactylifera L.) Improved by Coconut WaterBiotechnology, 2010, 9(4), 477-484.
High Frequency Plant Regeneration of a Dessert Banana Cv. Mehersagar for Commercial ExploitationBiotechnology, 2006, 5(3), 296-300.
In vitro Clonal Propagation of Cassia tora L. (Coffee Pod): A Medicinal PlantBiotechnology, 2011, 10(6), 546-550.
Micropropagation of Yam (Dioscorea rotundata): Assessment of Performance in Cassava Starch-gelled MediumBiotechnology, 2011, 10(6), 551-553.
In vitro Propagation of Ochlandra wightii (Munro) Fisch.: An Endemic Reed of Southern Western Ghats IndiaBiotechnology, 2012, 11(2), 67-73.
Effect of Abscisic Acid and Polyethylene Glycol on the Synchronization of Somatic Embryo Development in Date Palm (Phoenix dactylifera L.)Biotechnology, 2012, 11(6), 318-325.
Micropropagation of an Indian Ginger (Curcuma vamana Sabu and Mangaly): A Wild Relative of TurmericBiotechnology, 2012, 11(6), 333-338.
Regeneration of plantlets from node-derived callus in Aegle marmelos Corr.Biotechnology, 2007, 6(1), 72-75.
Rapid In vitro Clonal Propagation of Mantisia spathulata Schult, A Rare and Endemic Plant of Northeastern India for RecoveryBiotechnology, 2007, 6(1), 68-71.
Micropropagation of an Indian Ginger (Curcuma vamana Sabu and Mangaly): A Wild Relative of TurmericBiotechnology, 2012, 11(6), 333-338.
Cited By
Optimisation of Adventitious Shoot Regeneration and Agrobacterium-Mediated Transformation in Canna × generalis (Canna Lily)
Horticultural Plant Journal, 2018, (), . DOI: 10.1016/j.hpj.2018.11.002