Research Article
Pollen Parameters Estimates and Molecular Characterisation of Selected Varieties of Soybean [Glycine max (L.) Merril]

O.A.Y. Daudu, O.A. Falusi, A. Abubakar, V.O. Azuh, A.A. Gado and A.U. Ahiwe

Biotechnology, 2022, 21(1), 22-29.

Abstract

Background and Objective: Soybean (Glycine max) is a very important leguminous crop known for its highly valued protein and oil. Genetic variability is the basic requirement for crop improvement, it provides a wider scope for selection. Selection through studies of pollen behaviours and molecular characterisation increases the frequency for favourable alleles which can be explored in a breeding programme for the crop. Materials and Methods: Nineteen varieties of soybeans were collected from the gene bank of the International Institute of Tropical Agriculture (IITA), Ibadan. The varieties were evaluated for their pollen parameters following standard procedures. Results: Eleven distinct genotypes obtained from the pollen studies were further evaluated for genetic diversity using a simple sequence repeat (SSR) molecular marker. Most of these varieties were seen to be highly fertile (26.70-84.60%). The highest sterility value was recorded in TGX-2010-11F with a value of 73.3% and the least value of 15.4% was recorded in TGX-2018-5E. TGX-2007-3F produced significantly (p<0.05) highest pollen diameter (0.27 μ). Molecular diversity of 11 selected varieties from the initial twenty varieties using simple sequence repeat DNA marker generated 150-320 base pair with six primers. Genetic similarity among the varieties varies from 0.17-4.09 with an average gene diversity of 0.28. Clustered dendrogram of the 11 accessions revealed two major clades (TGX-1987-62F and TGX-2011-6F). Conclusion: The high genetic variability obtained for both pollen parameters and molecular characterisation indicate that soybean could be selected for those heritable traits and used as a tool for the improvement of the crop.

ASCI-ID: 11-911

Cited References Fulltext

Similar Articles


Plant Regeneration from the Cotyledonds of Tossa Jute (Corchorus olitorius L.)

Biotechnology, 2003, 2(3), 206-213.

In vitro Shoot Initiation from Nodal Explants of Jojoba (Simmondsia chinensis) Strains

Biotechnology, 2007, 6(2), 165-174.

In vitro Shoot Multiplication of Six Promising Strains of Jojoba (Simmondsia chinensis)

Biotechnology, 2007, 6(3), 309-315.

In vitro Root Formation in Micropropagated Shoots of Jojoba (Simmondsia chinensis)

Biotechnology, 2007, 6(4), 465-472.

Genetic Diversity of Wild and Cultivated Barley Genotypes Under Drought Stress Using RAPD Markers

Biotechnology, 2008, 7(4), 745-750.

Influence of Genotypes on Plant Regeneration from Cotyledons of Corchorus capsularis L.

Biotechnology, 2003, 2(1), 44-51.

Biochemical Properties of Some Thermophilic Lactic Acid Bacteria Strains from Traditional Fermented Milk Relevant to Their Technological Performance as Starter Culture

Biotechnology, 2007, 6(1), 14-21.

Detection of Genetically Modified Maize and Soybean Food Products in the Jordanian Market

Biotechnology, 2010, 9(4), 499-505.

Microbiological Assessment of Soya Milk Samples from Akoko Areas of Ondo State, Nigeria

Biotechnology, 2022, 21(3), 120-126.

Toward the Mapping of Agronomic Characters on a Rice Genetic Map: Quantitative Trait Loci Analysis under Saline Condition

Biotechnology, 2009, 8(1), 144-149.

Comparative Analysis of SSR and SRAP Sequence Divergence in Citrus Germplasm

Biotechnology, 2012, 11(1), 20-28.