Novel Approaches for Identification of Streptomyces noboritoensis TBG-V20 with Cellulase Production

Research Article
Novel Approaches for Identification of Streptomyces noboritoensis TBG-V20 with Cellulase Production

R. Arunachalam, E.G. Wesely, J. George and G. Annadurai

Current Research in Bacteriology, 2010, 3(1), 15-26.

Abstract

The present study was aimed at to identify the cellulase producing Actinomycetes from soil of Southwest ghats, Tamilnadu, India. The morphology, cultural, physiological and chemo taxonomical analysis and phylogenetic analysis of TBG-V20 were characterized. According to the morphological, isolate TBG-V20 was identified as a representative of the genus Streptomyces. The 16S rRNA region of this strain was amplified and sequenced. A Neighbor-Joining algorithm tree of 16S rRNA was constructed. In endocellulase activity, the Carboxy Methyl Cellulose (CMCase) activities of the strain TBG-V20 on eighth day an amount of 935 U L^-1 of glucose, 225 μg mL^-1 of protein and 870 mg/100 mL of growth (biomass) on tenth day were recorded. In exocellulase activity strain TBG-V20 on first day an amount of 500 μg mL^-1 glucose was produced. According to the observation and phylogenetic analysis, the strain TBG-V20 was proved to belong to the species Streptomyces noboritoensis with cellulase production.

ASCI-ID: 14-21

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