Research Article
Novel Approaches for Identification of Streptomyces noboritoensis TBG-V20 with Cellulase Production

R. Arunachalam, E.G. Wesely, J. George and G. Annadurai

Current Research in Bacteriology, 2010, 3(1), 15-26.


The present study was aimed at to identify the cellulase producing Actinomycetes from soil of Southwest ghats, Tamilnadu, India. The morphology, cultural, physiological and chemo taxonomical analysis and phylogenetic analysis of TBG-V20 were characterized. According to the morphological, isolate TBG-V20 was identified as a representative of the genus Streptomyces. The 16S rRNA region of this strain was amplified and sequenced. A Neighbor-Joining algorithm tree of 16S rRNA was constructed. In endocellulase activity, the Carboxy Methyl Cellulose (CMCase) activities of the strain TBG-V20 on eighth day an amount of 935 U L-1 of glucose, 225 μg mL-1 of protein and 870 mg/100 mL of growth (biomass) on tenth day were recorded. In exocellulase activity strain TBG-V20 on first day an amount of 500 μg mL-1 glucose was produced. According to the observation and phylogenetic analysis, the strain TBG-V20 was proved to belong to the species Streptomyces noboritoensis with cellulase production.

ASCI-ID: 14-21

Cited References Fulltext

Similar Articles

Characterization of Drug Resistant Bacteria, Conjugal Transfer Efficiency and their Growth Kinetics Against Cassia Plants Leaf Extract

Current Research in Bacteriology, 2015, 8(1), 1-10.

New Report on the Occurrence of Exiguobacterium sp. AT1b in the Persian Gulf and its Resistance to Mercury Pollution

Current Research in Bacteriology, 2011, 4(1), 23-27.

Antioxidant Activity of a Novel Streptomyces Strain Eri12 Isolated from the Rhizosphere of Rhizoma curcumae Longae

Current Research in Bacteriology, 2011, 4(2), 63-72.

Cited By

Influence of Carbon and Nitrogen Sources on the a-amylase Production by a Newly Isolated Thermophilic Streptomyces sp. MSC702 (MTCC 10772)

Asian Journal of Biotechnology, 2011, 3(6), 540. DOI: 10.3923/ajbkr.2011.540.553

Optimization of Culture Conditions Affecting Fungal Cellulase Production

Research Journal of Microbiology, 2012, 7(1), 23. DOI: 10.3923/jm.2012.23.31

Enzymatic Screening and Random Amplified Polymorphic DNA Fingerprinting of Soil Streptomycetes Isolated from Wayanad District in Kerala, India

Journal of Biological Sciences, 2012, 12(1), 43. DOI: 10.3923/jbs.2012.43.50

Systematic characterization of potential cellulolytic marine actinobacteria Actinoalloteichus sp. MHA15

Biotechnology Reports, 2017, 13(), 30. DOI: 10.1016/j.btre.2016.12.003

Actinomycetes benefaction role in soil and plant health

Microbial Pathogenesis, 2017, (), . DOI: 10.1016/j.micpath.2017.09.036

Optimization and characterization of biosurfactant from Streptomyces griseoplanus NRRL-ISP5009 (MS1)

Journal of Applied Microbiology, 2018, 124(3), 691. DOI: 10.1111/jam.13665

Screening and Molecular Characterization of Cellulase Producing Actinobacteria from Litchi Orchard

Current Chemical Biology, 2019, 13(1), 90. DOI: 10.2174/2212796812666180718114432

Bioethanol production from seagrass waste, through fermentation process using cellulase enzyme isolated from marine actinobacteria

Vegetos, 2021, (), . DOI: 10.1007/s42535-021-00239-5

Microbial Cell Factories Engineering for Production of Biomolecules

Microbial Cell Factories Engineering for Production of Biomolecules, 2021, (), 183. DOI: 10.1016/B978-0-12-821477-0.00011-8