A conserved transcriptional regulator is required for RNA-directed DNA methylation and plant development

X. J He, Y. F Hsu, S Zhu, H. L Liu, O Pontes, J Zhu, X Cui, C. S Wang and J. K. Zhu

Genes & Development, 2009, 23(23), 2717-2722. DOI: 10.1101/gad.1851809


RNA-directed DNA methylation (RdDM) is a conserved mechanism for epigenetic silencing of transposons and other repetitive elements. We report that the rdm4 (RNA-directed DNA Methylation4) mutation not only impairs RdDM, but also causes pleiotropic developmental defects in Arabidopsis. Both RNA polymerase II (Pol II)- and Pol V-dependent transcripts are affected in the rdm4 mutant. RDM4 encodes a novel protein that is conserved from yeast to humans and interacts with Pol II and Pol V in plants. Our results suggest that RDM4 functions in epigenetic regulation and plant development by serving as a transcriptional regulator for RNA Pol V and Pol II, respectively.

ASCI-ID: 1332-222