Response of Some Egyptian and Introduced Wheat Hybrids to Androgenic Process

Research Article
Response of Some Egyptian and Introduced Wheat Hybrids to Androgenic Process

Aida A. Rizkalla, A.M.F. Al-Ansary, S.A.A. Attia, A.A.A. Haiba and J.E. Nasseef

International Journal of Agricultural Research, 2012, 7(4), 205-214.

Abstract

Wheat anther culture produces homozygous doubled haploid plants, in a very short time span as comparing with traditional breeding methods. It is affected with several different factors, such as suitable spike stage as a donor for anthers, anthers cold pretreatment duration, rate of embryogenic callus formation and the time of anthers transferring from callus induction to regeneration medium. The effects of these factors on androgenic process were studied in this work the response of five F₁wheat hybrids, of ten varieties, differing in their salt tolerance, was investigated in anther culture system for haploid plantlets regeneration. Anthers were plated on P4S medium for callus induction, anthers were taken from different spikes ages (as in a distance between the top of the spike and flag leaf), different duration of cold pretreatment and different time of transferring anthers from callus induction to regeneration medium (190-2) were studied. Significant differences between genotypes on androgenic process were detected. Genotype influence was significant on the suitable spike length which has high percentage of proper stage of mid-uninucleate stage, consequently on both of callus induction and plant regeneration (green, albino and total plantlets). On the other hand, there was no significant influence in the embryogenic callus formation per 100 anthers between the hybrids under same duration (14, 28 and 56 days). The suitable duration for staying on induction callus medium was 28 days as the green regeneration plants increased. This is considered as accurate parameter for evaluation efficiency of androgensis for anther culture.

ASCI-ID: 32-508

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