Research Article
Measurement of an Optical Parameters: Absorption Scattering and Auto-florescence of Skin in vitro

S. Firdous, M. Ikram, M. Nawaz and M. Aslam

International Journal of Cancer Research, 2005, 1(1), 10-15.


In this study the chicken breast skin tissues were illuminated with collimated radiation of 400-700 nm Nd-YAG pumped dye laser and measured skin optical properties for dry and hydrated sample in vitro. Total reflectance and transmitted intensities were recorded by which scattering, absorption and anisotropic factors of the sample obtained using double integrating sphere setup. The in vitro optical parameters are higher then in vivo measurements. Our in vitro results are in agreements with other data available in literature. Hydration of skin is found to influence its scattering properties. Dry sample scatter less then hydrated sample. Skin auto-florescence spectra were acquired under different excitation wavelength, it shows difference between normal and malignant tissues.

ASCI-ID: 36-4

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