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Research Article
Bioenergetics of Binary Mixed Culture of Pseudomonas aeruginosa and Pseudomonas fluorescence Growth on Phenol in Aerobic Chemostat Culture

Samuel E. Agarry, Bamidele O. Solomon and Thomas O.K. Audu

International Journal of Chemical Technology, 2011, 3(1), 1-13.

Abstract

This study was carried out to estimate and evaluate the growth parameters of indigenous binary mixed culture of Pseudomonas aeruginosa and Pseudomonas fluorescence on phenol in an aerobic chemostat culture. The analysis was done by checking the obtained biodegradation data for consistency using carbon and available electron balances and estimating the growth parameters using two forms of growth models: reparameterized Pirt’s model based on Monod approach and a modified model based on substrate consumption rate being rate limiting. The results showed that the biodegradation data were consistent and the growth parameter estimates obtained using the two forms of models were similar. Using the reparameterized Pirt’s model coupled with covariate adjustment estimation technique (CAT), the best growth parameter estimates were the Maximum Likelihood Estimates (MLE) based on the complete data. The results also revealed that phenol could be oxidatively metabolized with a maximum of about 38-41% of the energy contained in it incorporated into binary mixed culture of P. aeruginosa and P. fluorescence biomass. The balance of 58-62% is mostly evolved as heat with little energy needed for maintenance of the cells. Thus, the study demonstrates that phenol supports the growth of Pseudomonas species.

ASCI-ID: 72-27

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