Research Article 
Effect of Exogenous Fibrolytic Enzymes on Ruminal Fermentation
and Gas Production by RUSITEC, in vitro Abomasum and Ileum
Digestibility
Mohamed Abdalla Elsiddig Mohamed, Cao Yangchun, Bello Musa Bodinga, Zheng Lixin, Yang Zekun, Liu Lihui, Junhu Yao, Rab Nawaz Soomro, Imtiaz Hussain Raja Abbasi and Li Wen
International Journal of Pharmacology, 2017, 13(8), 1020-1028.
Abstract
Background and Objective: Numerous in vitro studies have showed that exogenous fibrolytic enzyme can enhance fiber degradation of roughage. The aim of this study was to evaluate the effect of an Exogenous Fibrolytic Enzymes (EFE) product applied to a total mixed ration on ruminal fermentation, gas production and nutrient digestion in vitro whole digestive tract. Materials and Methods: The total mixed ration contained 55% concentrates, 22.5% corn silage and 22.5% alfalfa hay on a dry matter basis. The EFE multi enzyme feed additive powder produced from Ruminococcus flavefaciens was used in this study. Four levels of the EFE (0.0, 0.4, 0.8 and 1.2 g) were used E0, E2, E4 and E6, respectively, these levels of EFE were added directly into 20 g of diet in nylon bags and incubated in the RUSITEC for 48 h in vitro fermentation. Statistical analyses of continuous data were performed by use of SAS with repeated measures or Tukey’s test.Results: Results of the rumen experiment showed no significant difference (p>0.05) in pH-values, dry matter, organic matter digestibility, crude protein digestibility, gas production, CH4 and total volatile fatty acids production among all treatments in RUSITEC by using different EFE levels. The digestibility of neutral detergent fibre, acid detergent fiber and NH3-N concentrations were increased (p<0.05) with increasing EFE levels. The in vitro abomasum digestibility and total volatile fatty acids were unchanged (p>0.05). NH3-N concentrations were also increased (p<0.05) with increasing EFE levels. Moreover, in the in vitro abomasum experiment, the neutral detergent fibre digestibility and acid detergent fiber digestibility was increased (p<0.05) with increasing EFE levels. Conclusion: The results indicate that using high doses of EFE (VTR®) containing Ruminococcus flavefaciens in TMR (45:55) could improve the neutral detergent fibre digestibility and acid detergent fiber digestibility in rumen and small intestine.
ASCI-ID: 23-1209