Research Article
Effect of Organic Acids and Probiotics on Salmonella enteritidis Infection in Broiler Chickens

A. D. Wolfenden, J .L. Vicente, J. P. Higgins, R.L. Andreatti Filho, S. E. Higgins, B. M. Hargis and G. Tellez

International Journal of Poultry Science, 2007, 6(6), 403-405.

Abstract

The effect of an organic acid mixture (OA) and a Lactobacillus-based probiotic culture on Salmonella enteritidis (SE) infection in broiler chicks was evaluated. In exp. 1, chicks were challenged by oral gavage with SE, held in chick boxes for 2 h and randomly assigned to either untreated control or continuous OA treatment in the drinking water. Crop and cecal tonsils were cultured at 48 h and 5 d post-challenge for recovery of SE. Recovery of SE in the crop and cecal tonsils at 48 h was significantly (p<0.05) lower in the OA treated group as compared to control chickens but not different at 5d. In exps.2 and 3, chicks were SE challenged, held in chick boxes for 2 h and randomly assigned to either untreated control, probiotic, OA, or probiotic+OA. After 24 or 48 h, crop and cecal tonsils were cultured for the presence or absence of SE. After 24 h, probiotic or probiotic+OA significantly reduced SE recovery from the crop as compared to controls. All treatments reduced SE recovery from the cecal tonsils at 24 h. While no significant differences were observed in SE recovery from crop at 48 h, SE recovery from probiotic and or probiotic+OA groups was significantly lower than the controls in the cecal tonsils. These data suggest that combination treatment with the selected OA and Lactobacillus-based probiotic culture is more effective than individual treatment for Salmonella reduction in chicks.

ASCI-ID: 101-847

Cited References Fulltext

Similar Articles


Effects of Calsporin® on Turkey Performance, Carcass Yield and Nitrogen Reduction

International Journal of Poultry Science, 2004, 3(1), 75-79.

Inhibition of Growth of Escherichia coli, Salmonella typhimurium, and Clostridia perfringens on Chicken Feed Media by Lactobacillus salivarius and Lactobacillus plantarum

International Journal of Poultry Science, 2004, 3(9), 603-607.

Effect of Botanical Probiotic Containing Lactobacilli on Growth Performance and Populations of Bacteria in the Ceca, Cloaca, and Carcass Rinse of Broiler Chickens

International Journal of Poultry Science, 2006, 5(4), 344-350.

Effect of Lactobacillus Cultures and Oxytetracycline on the Growth Performance and Serum Lipids of Chickens

International Journal of Poultry Science, 2008, 7(4), 385-389.

Effect of a Lactic Acid Bacteria Based Probiotic, FloraMax-B11®, On Performance, Bone Qualities and Morphometric Analysis of Broiler Chickens: An Economic Analysis

International Journal of Poultry Science, 2013, 12(6), 322-327.

Characteristic of Lactobacillus Isolated from Pengging Duck's Intestines as Probiotics

International Journal of Poultry Science, 2014, 13(1), 47-51.

When Rooster Semen is Exposed to Lactobacillus Fertility is Reduced

International Journal of Poultry Science, 2015, 14(9), 541-547.

Effects of Organic Acid and Probiotics on Cecal Colonization and Immune Responses in Broiler Chickens Challenged with Salmonella Enteritidis

International Journal of Poultry Science, 2020, 19(1), 29-36.

The Effects of Antibiotic Growth Promoter, Probiotic or Organic Acid Supplementation on Performance, Intestinal Microflora and Tissue of Broilers

International Journal of Poultry Science, 2006, 5(2), 149-155.

Effect of Dietary Organic Acid Supplementation on Egg Production, Egg Quality and Some Blood Serum Parameters in Laying Hens

International Journal of Poultry Science, 2008, 7(6), 613-621.

The Evaluation of Organic Acids and Probiotic Cultures to Reduce Salmonella enteriditis Horizontal Transmission and Crop Infection in Broiler Chickens

International Journal of Poultry Science, 2007, 6(3), 182-186.

Use of Organic Acid, Herbs and Their Combination to Improve the Utilization of Commercial Low Protein Broiler Diets

International Journal of Poultry Science, 2009, 8(1), 14-20.

Egg Production and Calcium-Phosphorus Utilization of Laying Hens Fed Diets Supplemented with Phytase Alone or in Combination with Organic Acid

International Journal of Poultry Science, 2012, 11(3), 181-189.

Effect of Organic Acids on Salmonella Typhimurium Infection in Broiler Chickens

International Journal of Poultry Science, 2013, 12(2), 72-75.

Effects of Organic Acids on Salmonella enteritidis Growth Inhibition and Ileum Surface Area in Laying Ducks Fed Anaerobically Fermented Feed

International Journal of Poultry Science, 2017, 16(3), 98-104.

A Comparison of Essential Oil and Organic Acid Supplementation on the Live Performance and Intestinal Morphology of Broilers Fed a Diet Containing Palm Kernel Meal

International Journal of Poultry Science, 2017, 16(5), 196-202.

Comparison of Tributyrin and Coated Sodium Butyrate as Sources of Butyric Acid for Improvement of Growth Performance in Ross 308 Broilers

International Journal of Poultry Science, 2018, 17(6), 290-294.

Reduction of Broiler House Malodor by Direct Feeding of a Lactobacilli Containing Probiotic

International Journal of Poultry Science, 2003, 2(5), 313-317.

The Influence of Dietary Probiotic (Saccharomyces cerevisiae) Supplementation and Different Slaughter Age on the Performance, Slaughter and Carcass Properties of Broilers

International Journal of Poultry Science, 2005, 4(5), 309-316.

Effect of Probiotic Supplements on Egg Quality and Laying Hen`s Performance

International Journal of Poultry Science, 2005, 4(7), 488-492.

The Effects of Antibiotic Growth Promoter, Probiotic or Organic Acid Supplementation on Performance, Intestinal Microflora and Tissue of Broilers

International Journal of Poultry Science, 2006, 5(2), 149-155.

Effect of Probiotics on Broilers Performance

International Journal of Poultry Science, 2006, 5(6), 593-597.

Effect of Yoghurt and Protexin Boost on Broiler Performance

International Journal of Poultry Science, 2006, 5(7), 651-655.

Effect of Probiotic Thepax® and Saccharomyces cerevisiae Supplementation on Performance and Egg Quality of Laying Hens

International Journal of Poultry Science, 2007, 6(1), 52-54.

Effect of Dietary Inclusion of Cassava Yeast as Probiotic Source on Growth Performance, Small Intestine (Ileum) Morphology and Carcass Characteristic in Broilers

International Journal of Poultry Science, 2008, 7(3), 246-250.

Effects of Dietary Inclusion of Prebiotic, Probiotic and Synbiotic on the Intestinal Glucose Absorption of Broiler Chickens

International Journal of Poultry Science, 2008, 7(7), 686-691.

Prevention of Salmonella Infection in Poultry by Specific Egg-Derived Antibody

International Journal of Poultry Science, 2007, 6(4), 230-235.

Effects of a Probiotic and Other Feed Additives on Performance and Immune Response of Broiler Chicks

International Journal of Poultry Science, 2007, 6(4), 261-265.

The Evaluation of Organic Acids and Probiotic Cultures to Reduce Salmonella enteriditis Horizontal Transmission and Crop Infection in Broiler Chickens

International Journal of Poultry Science, 2007, 6(3), 182-186.

Effects of Cecal Cultures and Aspergillus Meal Prebiotic (Fermacto) on Growth Performance and Organ Weights of Broiler Chickens

International Journal of Poultry Science, 2007, 6(5), 340-344.

Metabolic and Physiological Impact of Probiotics or Direct-Fed-Microbials on Poultry: A Brief Review of Current Knowledge

International Journal of Poultry Science, 2007, 6(10), 694-704.

Effect of Different Levels of Protein and Protexin on Broiler Performance

International Journal of Poultry Science, 2007, 6(8), 573-577.

Evaluation of Spray Application of a Lactobacillus-based Probiotic on Salmonella enteritidis Colonization in Broiler Chickens

International Journal of Poultry Science, 2007, 6(7), 493-496.

The Influence of Biological Feed Additives on Broiler Performance

International Journal of Poultry Science, 2008, 7(9), 862-871.

Evaluation of Some Natural Feed Additive in Growing Chicks Diets

International Journal of Poultry Science, 2008, 7(9), 872-879.

Direct Fed Microbial, Primalac®, Supplementation and Jejunal Glucose and Proline Transport in Broiler Chickens

International Journal of Poultry Science, 2008, 7(12), 1163-1166.

Effect of Probiotic (Aspergillus niger) and Prebiotic (Taraxacum officinale) on Blood Picture and Biochemical Properties of Broiler Chicks

International Journal of Poultry Science, 2008, 7(12), 1182-1184.

Effect of Dietary Inclusion of Cassava Yeast as Probiotic Source on Egg Production and Egg Quality of Laying Hens

International Journal of Poultry Science, 2009, 8(2), 195-199.

Antibiotic Resistance in Poultry

International Journal of Poultry Science, 2009, 8(4), 404-408.

Performance and Economic Benefit of Broilers Fed Palm Kernel Cake-Based Diet Supplemented with Probiotic

International Journal of Poultry Science, 2009, 8(10), 1003-1005.

Performance of Single Comb White Leghorn Layers Fed with Aloe vera, Curcuma longa (Turmeric) and Probiotic

International Journal of Poultry Science, 2009, 8(8), 775-778.

Probiotic in Quail Nutrition: A Review

International Journal of Poultry Science, 2009, 8(12), 1218-1222.

Growth and Survival of Lactic Acid Bacteria Isolated from Byproduct of Virgin Coconut Oil as Probiotic Candidate for Poultry

International Journal of Poultry Science, 2011, 10(4), 309-314.

Effect of Probiotic on Some Physiological Parameters in Broiler Breeders

International Journal of Poultry Science, 2011, 10(8), 626-628.

The Effect of Single or Combined Dietary Supplementation of Mannan Oligosacharide and Probiotics on Performance and Slaughter Characteristics of Broilers

International Journal of Poultry Science, 2011, 10(11), 854-862.

The Probiotic Escherichia coli Nissle 1917 Enhances Early Gastrointestinal Maturation in Young Turkey Poults

International Journal of Poultry Science, 2012, 11(7), 445-452.

Effects of Bacillus subtilis in the Dynamics of Infiltration of Immunological Cells in the Intestinal Mucosa of Chickens Challenged with Salmonella Minnesota

International Journal of Poultry Science, 2012, 11(10), 630-634.

Effect of a Lactic Acid Bacteria Based Probiotic, FloraMax-B11®, On Performance, Bone Qualities and Morphometric Analysis of Broiler Chickens: An Economic Analysis

International Journal of Poultry Science, 2013, 12(6), 322-327.

Effect of Probiotic and Population Density on the Growth Performance and Carcass Characteristics in Broiler Chickens

International Journal of Poultry Science, 2013, 12(7), 390-395.

Effect of Dietary Supplementation with Probiotic or Essential Oils on Growth Performance of Broiler Chickens

International Journal of Poultry Science, 2013, 12(9), 538-544.

Effects of Enterococcus faecium on Diet in the Dynamics of CD4+ and CD8+ Cell Infiltration in the Intestinal Mucosa of Broilers Challenged with Salmonella Minnesota

International Journal of Poultry Science, 2013, 12(9), 523-528.

Effects of Dietary Inclusion of Probiotic or Prebiotic on Growth Performance, Organ Weight, Blood Parameters and Antibody Titers Against Influenza and Newcastle in Broiler Chickens

International Journal of Poultry Science, 2014, 13(2), 70-75.

Reducing the Toxicity of Aflatoxin in Broiler Chickens' Diet by Using Probiotic and Yeast

International Journal of Poultry Science, 2014, 13(7), 397-407.

Effect of Dry Probiotic Supplemented Fermented Feed on Production Performance of Akar Putra Chicken

International Journal of Poultry Science, 2015, 14(7), 420-426.

Welfare and Egg Production of Local Ducks Fed Diets Containing Two Probiotics in Commercial Farms

International Journal of Poultry Science, 2016, 15(6), 235-239.

Ability of Select Probiotics to Reduce Enteric Campylobacter Colonization in Broiler Chickens

International Journal of Poultry Science, 2017, 16(2), 37-42.

Evaluation of Bacteria from Gallus domesticus as a Potential Probiotic in Broiler Chicks: Effects on Growth Performance and Feed Conversion Ratio

International Journal of Poultry Science, 2017, 16(2), 43-49.

Inclusion of Probiotic on Chicken Performance and Immunity: A Review

International Journal of Poultry Science, 2017, 16(9), 328-335.

Effect of the Balance of Energy and Protein in Rations Given to Pitalah Ducks along with the Probiotic Bacillus amyloliquefaciens on the Live Weight, Percentage of Carcass, Percentage of Abdominal Fat and Income Over Feed Cost

International Journal of Poultry Science, 2017, 16(12), 500-505.

Effect of Direct-fed Microbials, Bacillus subtilis, on Production Performance, Serotonin Concentrations and Behavioral Parameters in a Selected Dominant Strain of White Leghorn Hens

International Journal of Poultry Science, 2018, 17(3), 106-115.

Measurement of in vitro Inhibition by Lactobacillus spp. Against Salmonella Heidelberg

International Journal of Poultry Science, 2018, 17(4), 184-188.

Impact of Feeding Fermented Wet Feed on Broiler Breeder Production Performance and Some Hatchability Traits

International Journal of Poultry Science, 2019, 18(3), 116-121.

Improvement of Small Intestine Morphometry in Broiler Chicken Using Fermented Cow and Soymilk as Probiotic

International Journal of Poultry Science, 2019, 18(6), 255-259.

Effect of Fermented Cow and Soymilk as Probiotic on Energy Metabolism and Nutrient Retention in Broiler Chicken

International Journal of Poultry Science, 2020, 19(6), 277-281.

Effects of Dietary Supplementation of a Single-and a Multi-Strain Probiotic on Growth Performance and Intestinal Histomorphology of Commercial Broiler Chickens

International Journal of Poultry Science, 2020, 19(8), 363-371.

Effects of Megasphaera elsdenii on Growth Performance and Characteristics of Cecal Digesta in Broiler Chickens

International Journal of Poultry Science, 2020, 19(7), 321-329.

Effect of Dietary Supplementation of Probiotic Bacteria Obtained from Fermented Tchoukoutou on the Production Performance of Local and Exotic Guinea Fowl

International Journal of Poultry Science, 2021, 20(5), 224-230.

Probiotic Potential of Lactic Acid Bacteria Isolated from Broiler Chickens in Côte d’Ivoire

International Journal of Poultry Science, 2022, 21(3), 119-128.

The Isolation of Antibiotic-Resistant Salmonella from Intestine and Liver of Poultry in Shiraz Province of Iran

International Journal of Poultry Science, 2005, 4(5), 320-322.

Epidemiological Characterization of Avian Salmonella enterica Serovar Infections in India

International Journal of Poultry Science, 2005, 4(6), 388-395.

Detection of InvA Gene in Isolated Salmonella from Broilers by PCR Method

International Journal of Poultry Science, 2005, 4(8), 557-559.

Seroprevalence of Salmonella and Mycoplasma gallisepticum Infection in the Six Model Breeder Poultry Farms at Patuakhali District in Bangladesh

International Journal of Poultry Science, 2005, 4(11), 905-910.

Microbiology of Contaminated or Visibly Clean Broiler Carcasses Processed with an Inside-Outside Bird Washer

International Journal of Poultry Science, 2005, 4(12), 955-958.

Antimicrobial Resistance Patterns of Salmonella from Retail Chicken

International Journal of Poultry Science, 2006, 5(4), 351-354.

An Investigation into Salmonella and Fecal Coliform Contamination of Drinking Water in Broiler Farms in Iran

International Journal of Poultry Science, 2006, 5(5), 491-493.

Bacteriological Quality of Cracked Eggs Sold for Consumption in Abeokuta, Nigeria

International Journal of Poultry Science, 2006, 5(8), 772-775.

Microbial Efficacy of Commercial Application of Cecure® CPC Antimicrobial to Ingesta-Contaminated Pre-Chill Broiler Carcasses

International Journal of Poultry Science, 2006, 5(8), 698-703.

Influence of Grain Particle Size and Insoluble Fiber Content on Salmonella Colonization and Shedding of Turkeys Fed Corn-Soybean Meal Diets

International Journal of Poultry Science, 2006, 5(8), 731-739.

Coliforms, Escherichia coli, Campylobacter, and Salmonella in a Counterflow Poultry Scalder with a Dip Tank

International Journal of Poultry Science, 2006, 5(9), 846-849.

Characterization of Salmonella from Three Commercial North Carolina Broiler Farms

International Journal of Poultry Science, 2006, 5(12), 1102-1109.

Humoral and Mucosal-Humoral Immune Response to a Salmonella vaccination Program in Broiler Breeders

International Journal of Poultry Science, 2007, 6(3), 172-181.

The Evaluation of Organic Acids and Probiotic Cultures to Reduce Salmonella enteriditis Horizontal Transmission and Crop Infection in Broiler Chickens

International Journal of Poultry Science, 2007, 6(3), 182-186.

Intestinal Humoral Immune Response and Resistance to Salmonella Challenge of Progeny from Breeders Vaccinated with Killed Antigen

International Journal of Poultry Science, 2007, 6(6), 417-423.

An Investigation into Salmonella Infection Status in Backyard Chickens in Iran

International Journal of Poultry Science, 2007, 6(3), 227-229.

Use of Wide-Host-Range Bacteriophages to Reduce Salmonella on Poultry Products

International Journal of Poultry Science, 2007, 6(10), 754-757.

Evaluation of Alternative Host Bacteria as Vehicles for Oral Administration of Bacteriophages

International Journal of Poultry Science, 2007, 6(10), 758-761.

Internal and External Carriage of Inoculated Salmonella in Broiler Chickens

International Journal of Poultry Science, 2007, 6(12), 952-954.

Preliminary Investigations on Salmonella spp. Incidence in Meat Chicken Farms in Italy, Germany, Lithuania and the Netherlands

International Journal of Poultry Science, 2008, 7(8), 813-817.

Risk Factors for the Presence of Campylobacter Sp. in Lithuanian Broiler Flocks

International Journal of Poultry Science, 2008, 7(12), 1242-1246.

Epidemiological Surveillance on Environmental Contaminants in Poultry Farms

International Journal of Poultry Science, 2009, 8(2), 151-155.

Presence of Interferon-Gamma and IL-2 in Supernatants of Salmonella enteritidis-Immune Lymphokines

International Journal of Poultry Science, 2009, 8(9), 820-823.

Antimicrobial Activity of Commercial Concrete Sealant Against Salmonella Spp: A Model for Poultry Processing Plants

International Journal of Poultry Science, 2009, 8(10), 939-945.

Efficacy of a Post-Chill Precure™ Treatment1 (pH = 1.5) for Reduction of Salmonella on Inoculated Broilers

International Journal of Poultry Science, 2010, 9(1), 97-97.

Efficacy of the Precure™ Antimicrobial1 at Various pH Values for Controlling Microorganisms on Broiler Paws

International Journal of Poultry Science, 2010, 9(1), 98-98.

Efficacy of the FreshLight® 210 Ultraviolet Light System1 for Control and Elimination of Salmonella typhimurium and Listeria innocua in a Commercial Poultry Marinade (Flow Rate = 20 GPM and Turnover Time = 1 minute)

International Journal of Poultry Science, 2010, 9(1), 100-100.

Evaluation of a Screening and Selection Method for Bacillus Isolates for Use as Effective Direct-fed Microbials in Commercial Poultry

International Journal of Poultry Science, 2010, 9(4), 317-323.

Rapid Detection of Salmonella in Chicken Meat Using Immunomagnetic Separation, CHROMagar, ELISA and Real-time Polymerase Chain Reaction (RT-PCR)

International Journal of Poultry Science, 2010, 9(9), 831-835.

Development and Evaluation of an Δ aroA / Δ htrA Salmonella enteritidis Vector Expressing Eimeria maxima TRAP Family Protein EmTFP250 with CD 154 (CD 40L) as Candidate Vaccines against Coccidiosis in Broilers

International Journal of Poultry Science, 2010, 9(11), 1031-1037.

Detection of Salmonella and Escherichia coli in Egg Shell and Egg Content from Different Housing Systems for Laying Hens

International Journal of Poultry Science, 2011, 10(2), 93-97.

Zeolite as a Feed Additive to Reduce Salmonella and Improve Production Performance in Broilers

International Journal of Poultry Science, 2011, 10(6), 448-454.

Supplementation of Feed Grade Sodium Bisulfate in Broiler Diets Improves Feed Efficiency

International Journal of Poultry Science, 2011, 10(9), 670-676.

Effect of Ultrasonic Marination on Broiler Breast Meat Quality and Salmonella Contamination

International Journal of Poultry Science, 2011, 10(10), 757-759.

Evaluation of an Alcohol-based Sanitizer Spray’s Bactericidal Effects on Salmonella Inoculated onto Stainless Steel and Shell Egg Processing Equipment

International Journal of Poultry Science, 2012, 11(2), 92-95.

Live Performance Characteristics, Pathogen Load and Foot Pad Lesions in Range-Reared Heritage vs. Conventional Turkeys (Meleagris gallopavo)

International Journal of Poultry Science, 2012, 11(7), 438-444.

Effect of Adding Various Organic Acids During the Feed Withdrawal Period on Salmonella Reduction in Broilers

International Journal of Poultry Science, 2012, 11(7), 482-487.

Effect of an Acid Antimicrobial on Turkey Breast Meat Salmonella and Total Aerobes

International Journal of Poultry Science, 2012, 11(11), 673-676.

Effect of Organic Acids on Salmonella Typhimurium Infection in Broiler Chickens

International Journal of Poultry Science, 2013, 12(2), 72-75.

Reduction of Salmonella and Ammonia Emissions in Broiler Litter Using Sulfuric Acid and Aluminum Sulfate

International Journal of Poultry Science, 2013, 12(6), 328-334.

Effect of Chitosan as a Biological Sanitizer for Salmonella Typhimurium and Aerobic Gram Negative Spoilage Bacteria Present on Chicken Skin

International Journal of Poultry Science, 2013, 12(6), 318-321.

Effects of Enterococcus faecium on Diet in the Dynamics of CD4+ and CD8+ Cell Infiltration in the Intestinal Mucosa of Broilers Challenged with Salmonella Minnesota

International Journal of Poultry Science, 2013, 12(9), 523-528.

Bacterial Content in Runoff from Simulated Rainfall Applied to Plots Amended with Poultry Litter

International Journal of Poultry Science, 2014, 13(3), 133-137.

Improved Detection of Campylobacter jejuni, Listeria monocytogenes and Salmonella Typhimurium from Raw Meat Products Using Conventional and Newly Developed TaqMan Assays

International Journal of Poultry Science, 2015, 14(7), 364-375.

Detection of Antimicrobial Phenotypes, β-Lactamase Encoding Genes and Class I Integrons in Salmonella Serovars Isolated from Broilers

International Journal of Poultry Science, 2016, 15(1), 1-7.

Analysis of Salmonella Contamination in Poultry Meat at Various Retailing, Different Storage Temperatures and Carcass Cuts - A Literature Survey

International Journal of Poultry Science, 2016, 15(3), 111-120.

Rugose Morphotype of Salmonella enterica Serovar Typhimurium ATCC14028 Exhibits Chlorine Resistance and Strong Biofilm Forming Ability

International Journal of Poultry Science, 2018, 17(6), 295-305.

Persistence of Campylobacter and Salmonella in the Ceca, Spleen and Liver/gallbladder of Inoculated Broilers

International Journal of Poultry Science, 2018, 17(8), 374-377.

Effect of Light Spectrum on Stress Susceptibility and Salmonella Status of Laying Hens

International Journal of Poultry Science, 2018, 17(11), 529-535.

Association of the Toll-like Receptor 4 (TLR4) and Myxovirus (Mx) Genes With Resistance to Salmonella and Newcastle Disease in Selected Sentul Chickens

International Journal of Poultry Science, 2018, 17(12), 591-599.