Research Article 
Development and Evaluation of an Δ aroA / Δ htrA Salmonella enteritidis Vector Expressing Eimeria maxima TRAP Family Protein EmTFP250 with CD 154 (CD 40L) as Candidate Vaccines against Coccidiosis in Broilers
S. Shivaramaiah, J.R. Barta, S.L. Layton, C. Lester, Y.M. Kwon, L.R. Berghman, B.M. Hargis and G. Tellez
International Journal of Poultry Science, 2010, 9(11), 1031-1037.
Abstract
Coccidiosis is caused by parasites of the genus Eimeria, belonging to phylum Apicomplexa. EmTFP250 is a high molecular mass, asexual stage antigen from Eimeria maxima (EM) strongly associated with maternal immunity in newly hatched chickens. Cloning and sequence analysis predict the antigen to be a novel member of the Thrombospondin-Related Adhesive Protein (TRAP) family. Three novel attenuated Salmonella enteritidis strains (ΔSE) expressing TRAP oligopeptides in association with a potential immune-enhancing CD 154 sequence, on the outer membrane protein lamB, were developed. Broiler chicks were grouped based on treatment and 108 cfu/chick of vectors expressing one of three sequences, or vehicle alone, was orally administered to each group. At 21 d of age, all groups were challenged with 104 sporulated oocysts/chick orally. Mortality at 5d post-challenge was markedly different (p<0.05) in chickens vaccinated with TRAP Upstream (US). To further evaluate the efficacy of TRAP US as a potential vaccine candidate, a similar study was conducted. Broilers were orally vaccinated with 108 cfu/chick vehicle with TRAP US and CD 154 or sham vaccinated with saline. Coccidia challenge was performed with 105 sporulated oocysts/chick at 22 d of age. Immunized chickens showed remarkable improvement in weight gain (p<0.05) and had reduced mortality (p = 0.055) when compared to non-immunized controls. These two studies underscore the potential of EmTFP250 as a potential candidate for a recombinant vaccine targeting coccidiosis in chickens.
ASCI-ID: 101-1477
Similar Articles
Comparative Efficacy of Different Feed Additive Anticoccidials in Broilers
International Journal of Poultry Science, 2002, 1(6), 185-187.
Prevalence and Economic Losses Due to Infectious Bursal Disease in Broilers in Mirpur and Kotli Districts of KashmirInternational Journal of Poultry Science, 2003, 2(4), 267-270.
Prevalence and Management Issues Associated with Poultry Coccidiosis in Abak Agricultural Zone of Akwa Ibom State, NigeriaInternational Journal of Poultry Science, 2004, 3(2), 135-139.
Assessment of Economic Impact Caused by Poultry Coccidiosis in Small and Large Scale Poultry Farms in Debre Zeit, EthiopiaInternational Journal of Poultry Science, 2004, 3(11), 715-718.
Association Between Coccidia and Intestinal Helminths in Broiler ChickensInternational Journal of Poultry Science, 2008, 7(1), 36-39.
Prevalence and Identification of Species of Eimeria Causing Coccidiosis in Poultry Within Vom, Plateau State, NigeriaInternational Journal of Poultry Science, 2008, 7(9), 917-918.
Comparing the Feeding of Fungus Myceliated Grain with Other Anticoccidial Control Measures on Oocyst Excretion of Eimeria Challenged BroilersInternational Journal of Poultry Science, 2010, 9(7), 648-651.
Outbreak of Coccidiosis in 5-day Old Commercial Broiler Breeder Flock in Zaria, NigeriaInternational Journal of Poultry Science, 2010, 9(12), 1112-1115.
Evaluation of Quebracho Red Wood (Schinopsis lorentzii) Polyphenolic Vegetable Extract for the Reduction of Coccidiosis in Broiler ChicksInternational Journal of Poultry Science, 2011, 10(5), 344-349.
Evaluating Different Vaccine Routes Against CoccidiosisInternational Journal of Poultry Science, 2009, 8(10), 980-984.
Effect of Different Mushrooms Fed to Eimeria-Challenged Broilers on Rearing PerformanceInternational Journal of Poultry Science, 2012, 11(7), 433-437.
Effect of Eimeria Infection on Cecal Microbiome of Broilers Fed Essential OilsInternational Journal of Poultry Science, 2012, 11(12), 747-755.
High Levels of Zinc Stimulate Different Aspects of Immune System in Broiler ChicksInternational Journal of Poultry Science, 2013, 12(2), 94-97.
Examination of a Novel Practical Poultry Management Method to Enhance the Effect of Live Eimeria Vaccination for Conventionally Housed Replacement Layer PulletsInternational Journal of Poultry Science, 2013, 12(3), 175-184.
Pyrosequencing Analysis of Salinomycin and Eimeria spp. Challenge-Induced Changes in Broiler Cecal Microbial CommunitiesInternational Journal of Poultry Science, 2013, 12(12), 689-697.
Coccidiosis Prevalence and Correlation with Intestinal Health of Broilers in Brazilian Agricultural Industries Between the Years 2012 and 2014International Journal of Poultry Science, 2015, 14(9), 511-515.
Haplotype Structure and DNA Sequence Variation of the Liver Expressed Antimicrobial Peptide-2 (chLEAP-2) Gene in Chickens challenged with Eimeria maximaInternational Journal of Poultry Science, 2017, 16(9), 336-343.
Study of the Correlation Between Intestinal Health and Prevalence of Coccidiosis in Broiler Chickens of Brazilian Agribusinesses Between the Years 2015 and 2016International Journal of Poultry Science, 2017, 16(10), 381-386.
Occurrence of Necrotic Enteritis (NE) Among Local Chickens Slaughtered at Muda Lawal Market in Bauchi Metropolis, Bauchi State, NigeriaInternational Journal of Poultry Science, 2020, 19(11), 498-502.
Aqueous Extracts of Aloe vera and Carica papaya Leaves: Impacts on Coccidiosis, Production and Haematology in Kabir Chickens in Buea, South West CameroonInternational Journal of Poultry Science, 2021, 20(3), 106-115.
CorrigendumAqueous Extracts of Aloe vera and Carica papaya Leaves: Impacts on Coccidiosis, Production and Haematology in Kabir Chickens in Buea, South West Cameroon
International Journal of Poultry Science, 2021, 20(3), 145-145.
The Influence of a Quillaja and Yucca Combination on Growth Performance and Lesion Scores of Broilers Administered Chemical Anticoccidials or a Live Coccidiosis VaccineInternational Journal of Poultry Science, 2022, 21(2), 50-56.
An Epizootic of Combined Clostridium perfringens, Eimeria Spp. and Capillaria Spp. Enteritis and Histomonas Spp. Hepatitis with Escherichia coli Septicemia in Bobwhite Quails (Colinus virginianus)International Journal of Poultry Science, 2004, 3(7), 438-441.
Assessment of Economic Impact Caused by Poultry Coccidiosis in Small and Large Scale Poultry Farms in Debre Zeit, EthiopiaInternational Journal of Poultry Science, 2004, 3(11), 715-718.
Study on Poultry Coccidiosis in Tiyo District, Arsi Zone, EthiopiaInternational Journal of Poultry Science, 2008, 7(3), 251-256.
Sources and Routes of Introduction of Eimeria Oocysts into Broiler Chick`s HousesInternational Journal of Poultry Science, 2007, 6(12), 925-927.
Effect of Phase Feeding Supplemental Fungus Myceliated Grain on Oocyst Excretion and Performance of Broiler ChickensInternational Journal of Poultry Science, 2011, 10(1), 1-3.
Coccidiosis Prevalence and Correlation with Intestinal Health of Broilers in Brazilian Agricultural Industries Between the Years 2012 and 2014International Journal of Poultry Science, 2015, 14(9), 511-515.
Study of the Correlation Between Intestinal Health and Prevalence of Coccidiosis in Broiler Chickens of Brazilian Agribusinesses Between the Years 2015 and 2016International Journal of Poultry Science, 2017, 16(10), 381-386.
Combination of Xylanase and Bacillus Direct-fed Microbials, as an Alternative to Antibiotic Growth Promoters, Improves Live Performance and Gut Health in Subclinical Challenged BroilersInternational Journal of Poultry Science, 2018, 17(8), 362-366.
The Isolation of Antibiotic-Resistant Salmonella from Intestine and Liver of Poultry in Shiraz Province of IranInternational Journal of Poultry Science, 2005, 4(5), 320-322.
Epidemiological Characterization of Avian Salmonella enterica Serovar Infections in IndiaInternational Journal of Poultry Science, 2005, 4(6), 388-395.
Detection of InvA Gene in Isolated Salmonella from Broilers by PCR MethodInternational Journal of Poultry Science, 2005, 4(8), 557-559.
Seroprevalence of Salmonella and Mycoplasma gallisepticum Infection in the Six Model Breeder Poultry Farms at Patuakhali District in BangladeshInternational Journal of Poultry Science, 2005, 4(11), 905-910.
Microbiology of Contaminated or Visibly Clean Broiler Carcasses Processed with an Inside-Outside Bird WasherInternational Journal of Poultry Science, 2005, 4(12), 955-958.
Antimicrobial Resistance Patterns of Salmonella from Retail ChickenInternational Journal of Poultry Science, 2006, 5(4), 351-354.
An Investigation into Salmonella and Fecal Coliform Contamination of Drinking Water in Broiler Farms in IranInternational Journal of Poultry Science, 2006, 5(5), 491-493.
Bacteriological Quality of Cracked Eggs Sold for Consumption in Abeokuta, NigeriaInternational Journal of Poultry Science, 2006, 5(8), 772-775.
Microbial Efficacy of Commercial Application of Cecure® CPC Antimicrobial to Ingesta-Contaminated Pre-Chill Broiler CarcassesInternational Journal of Poultry Science, 2006, 5(8), 698-703.
Influence of Grain Particle Size and Insoluble Fiber Content on Salmonella Colonization and Shedding of Turkeys Fed Corn-Soybean Meal DietsInternational Journal of Poultry Science, 2006, 5(8), 731-739.
Coliforms, Escherichia coli, Campylobacter, and Salmonella in a Counterflow Poultry Scalder with a Dip TankInternational Journal of Poultry Science, 2006, 5(9), 846-849.
Characterization of Salmonella from Three Commercial North Carolina Broiler FarmsInternational Journal of Poultry Science, 2006, 5(12), 1102-1109.
Humoral and Mucosal-Humoral Immune Response to a Salmonella vaccination Program in Broiler BreedersInternational Journal of Poultry Science, 2007, 6(3), 172-181.
The Evaluation of Organic Acids and Probiotic Cultures to Reduce Salmonella enteriditis Horizontal Transmission and Crop Infection in Broiler ChickensInternational Journal of Poultry Science, 2007, 6(3), 182-186.
Effect of Organic Acids and Probiotics on Salmonella enteritidis Infection in Broiler ChickensInternational Journal of Poultry Science, 2007, 6(6), 403-405.
Intestinal Humoral Immune Response and Resistance to Salmonella Challenge of Progeny from Breeders Vaccinated with Killed AntigenInternational Journal of Poultry Science, 2007, 6(6), 417-423.
An Investigation into Salmonella Infection Status in Backyard Chickens in IranInternational Journal of Poultry Science, 2007, 6(3), 227-229.
Use of Wide-Host-Range Bacteriophages to Reduce Salmonella on Poultry ProductsInternational Journal of Poultry Science, 2007, 6(10), 754-757.
Evaluation of Alternative Host Bacteria as Vehicles for Oral Administration of BacteriophagesInternational Journal of Poultry Science, 2007, 6(10), 758-761.
Internal and External Carriage of Inoculated Salmonella in Broiler ChickensInternational Journal of Poultry Science, 2007, 6(12), 952-954.
Preliminary Investigations on Salmonella spp. Incidence in Meat Chicken Farms in Italy, Germany, Lithuania and the NetherlandsInternational Journal of Poultry Science, 2008, 7(8), 813-817.
Risk Factors for the Presence of Campylobacter Sp. in Lithuanian Broiler FlocksInternational Journal of Poultry Science, 2008, 7(12), 1242-1246.
Epidemiological Surveillance on Environmental Contaminants in Poultry FarmsInternational Journal of Poultry Science, 2009, 8(2), 151-155.
Presence of Interferon-Gamma and IL-2 in Supernatants of Salmonella enteritidis-Immune LymphokinesInternational Journal of Poultry Science, 2009, 8(9), 820-823.
Antimicrobial Activity of Commercial Concrete Sealant Against Salmonella Spp: A Model for Poultry Processing PlantsInternational Journal of Poultry Science, 2009, 8(10), 939-945.
Efficacy of a Post-Chill Precure™ Treatment1 (pH = 1.5) for Reduction of Salmonella on Inoculated BroilersInternational Journal of Poultry Science, 2010, 9(1), 97-97.
Efficacy of the Precure™ Antimicrobial1 at Various pH Values for Controlling Microorganisms on Broiler PawsInternational Journal of Poultry Science, 2010, 9(1), 98-98.
Efficacy of the FreshLight® 210 Ultraviolet Light System1 for Control and Elimination of Salmonella typhimurium and Listeria innocua in a Commercial Poultry Marinade (Flow Rate = 20 GPM and Turnover Time = 1 minute)International Journal of Poultry Science, 2010, 9(1), 100-100.
Evaluation of a Screening and Selection Method for Bacillus Isolates for Use as Effective Direct-fed Microbials in Commercial PoultryInternational Journal of Poultry Science, 2010, 9(4), 317-323.
Rapid Detection of Salmonella in Chicken Meat Using Immunomagnetic Separation, CHROMagar, ELISA and Real-time Polymerase Chain Reaction (RT-PCR)International Journal of Poultry Science, 2010, 9(9), 831-835.
Detection of Salmonella and Escherichia coli in Egg Shell and Egg Content from Different Housing Systems for Laying HensInternational Journal of Poultry Science, 2011, 10(2), 93-97.
Zeolite as a Feed Additive to Reduce Salmonella and Improve Production Performance in BroilersInternational Journal of Poultry Science, 2011, 10(6), 448-454.
Supplementation of Feed Grade Sodium Bisulfate in Broiler Diets Improves Feed EfficiencyInternational Journal of Poultry Science, 2011, 10(9), 670-676.
Effect of Ultrasonic Marination on Broiler Breast Meat Quality and Salmonella ContaminationInternational Journal of Poultry Science, 2011, 10(10), 757-759.
Evaluation of an Alcohol-based Sanitizer Spray’s Bactericidal Effects on Salmonella Inoculated onto Stainless Steel and Shell Egg Processing EquipmentInternational Journal of Poultry Science, 2012, 11(2), 92-95.
Live Performance Characteristics, Pathogen Load and Foot Pad Lesions in Range-Reared Heritage vs. Conventional Turkeys (Meleagris gallopavo)International Journal of Poultry Science, 2012, 11(7), 438-444.
Effect of Adding Various Organic Acids During the Feed Withdrawal Period on Salmonella Reduction in BroilersInternational Journal of Poultry Science, 2012, 11(7), 482-487.
Effect of an Acid Antimicrobial on Turkey Breast Meat Salmonella and Total AerobesInternational Journal of Poultry Science, 2012, 11(11), 673-676.
Effect of Organic Acids on Salmonella Typhimurium Infection in Broiler ChickensInternational Journal of Poultry Science, 2013, 12(2), 72-75.
Reduction of Salmonella and Ammonia Emissions in Broiler Litter Using Sulfuric Acid and Aluminum SulfateInternational Journal of Poultry Science, 2013, 12(6), 328-334.
Effect of Chitosan as a Biological Sanitizer for Salmonella Typhimurium and Aerobic Gram Negative Spoilage Bacteria Present on Chicken SkinInternational Journal of Poultry Science, 2013, 12(6), 318-321.
Effects of Enterococcus faecium on Diet in the Dynamics of CD4+ and CD8+ Cell Infiltration in the Intestinal Mucosa of Broilers Challenged with Salmonella MinnesotaInternational Journal of Poultry Science, 2013, 12(9), 523-528.
Bacterial Content in Runoff from Simulated Rainfall Applied to Plots Amended with Poultry LitterInternational Journal of Poultry Science, 2014, 13(3), 133-137.
Improved Detection of Campylobacter jejuni, Listeria monocytogenes and Salmonella Typhimurium from Raw Meat Products Using Conventional and Newly Developed TaqMan AssaysInternational Journal of Poultry Science, 2015, 14(7), 364-375.
Detection of Antimicrobial Phenotypes, β-Lactamase Encoding Genes and Class I Integrons in Salmonella Serovars Isolated from BroilersInternational Journal of Poultry Science, 2016, 15(1), 1-7.
Analysis of Salmonella Contamination in Poultry Meat at Various Retailing, Different Storage Temperatures and Carcass Cuts - A Literature SurveyInternational Journal of Poultry Science, 2016, 15(3), 111-120.
Rugose Morphotype of Salmonella enterica Serovar Typhimurium ATCC14028 Exhibits Chlorine Resistance and Strong Biofilm Forming AbilityInternational Journal of Poultry Science, 2018, 17(6), 295-305.
Persistence of Campylobacter and Salmonella in the Ceca, Spleen and Liver/gallbladder of Inoculated BroilersInternational Journal of Poultry Science, 2018, 17(8), 374-377.
Effect of Light Spectrum on Stress Susceptibility and Salmonella Status of Laying HensInternational Journal of Poultry Science, 2018, 17(11), 529-535.
Association of the Toll-like Receptor 4 (TLR4) and Myxovirus (Mx) Genes With Resistance to Salmonella and Newcastle Disease in Selected Sentul ChickensInternational Journal of Poultry Science, 2018, 17(12), 591-599.
Role of Maternal Antibodies in Protection Against Infectious Bursal Disease in Commercial BroilersInternational Journal of Poultry Science, 2003, 2(4), 251-255.
Evaluation of Different Programs of Newcastle Disease Vaccination in Japanese Quail (Coturnix coturnix japonica)International Journal of Poultry Science, 2004, 3(5), 354-356.
Effects of Vaccination Routes Against IB on Performance and Immune Responses of Broiler ChickensInternational Journal of Poultry Science, 2005, 4(10), 795-798.
Study of Experimental Vaccination in White Pekin Duck (Anas platyrhynchos) Against Newcastle Disease: Investigation of the State of Virus CarrierInternational Journal of Poultry Science, 2006, 5(8), 786-788.
Clinical and Immunological Parameters of Newcastle Disease Vaccination in Juvenile Ring - Necked Pheasants (Phasianus colchicus)International Journal of Poultry Science, 2008, 7(3), 283-284.
Clinical and Immunological Parameters of Newcastle Disease Vaccination in Chinese Goose (Anser cygnoides)International Journal of Poultry Science, 2008, 7(7), 676-677.
National Vaccination Campaigns Against Highly Pathogenic Avian Influenza Outbreaks in Developing NationsInternational Journal of Poultry Science, 2007, 6(7), 531-534.
Effect of Levamisole as an Immunomodulator in Cockerels Vaccinated with Newcastle Disease VaccineInternational Journal of Poultry Science, 2008, 7(11), 1042-1044.
Adoption of Vaccination Against Newcastle Disease by Rural Poultry Women Farmers in the North Central Zone of NigeriaInternational Journal of Poultry Science, 2009, 8(5), 500-503.
Response to Experimental Vaccination Against Newcastle Disease in Domestic Ducks (Anas platyrhynchos): Clinical and Immunological ParametersInternational Journal of Poultry Science, 2009, 8(10), 963-965.
Evaluating Different Vaccine Routes Against CoccidiosisInternational Journal of Poultry Science, 2009, 8(10), 980-984.
Evaluation of Experimental Vaccination in Chinese Goose (Anser cygnoides) Against Newcastle Disease: Investigation of the State of Virus CarrierInternational Journal of Poultry Science, 2009, 8(5), 512-514.
Experimental Vaccination Against Newcastle Disease in Japanese Quails (Coturnix coturnix japonica): Clinical and Immunological ParametersInternational Journal of Poultry Science, 2009, 8(1), 52-54.
Immunological and Clinical Parameters of Newcastle Disease Vaccination in Bronze Turkeys (Meleagris gallopavo)International Journal of Poultry Science, 2010, 9(2), 180-182.
Effects of Vaccination with Lentogenic Vaccine and Challenge with Virulent Newcastle Disease Virus (NDV) on Egg Production in Commercial and SPF ChickensInternational Journal of Poultry Science, 2011, 10(2), 98-105.
Analysis of the Effect of Diluent for Rehydration of PoulVac® MycoF on Vaccination Seroconversion ResultsInternational Journal of Poultry Science, 2011, 10(5), 397-400.
Evaluation of Experimental Vaccination Against Newcastle Disease in Lovebirds (Agapornis roseicollis): Investigation of the State of Virus CarrierInternational Journal of Poultry Science, 2011, 10(9), 694-696.
Investigation of the State of Virus Carrier of Newcastle Disease in Budgerigars (Melopsittacus undulatus)International Journal of Poultry Science, 2013, 12(3), 135-137.
Humoral Antibody Immune Response of Newcastle Disease Vaccination in Lovebirds (Agapornis roseicollis)International Journal of Poultry Science, 2013, 12(3), 138-140.
Vaccination Against Infectious Bursal Disease Virus (IBDV) in Broiler Birds in Mozambique: Are We Doing Enough?International Journal of Poultry Science, 2015, 14(12), 644-646.
Phytogenic Feed Additive Enhance Innate and Humoral Immune Response to Newcastle Disease Virus Vaccination in Broiler ChickensInternational Journal of Poultry Science, 2019, 18(2), 93-100.
In vivo and In vitro Inoculations of Live Viruses Alter Parthenogenesis in Chinese Painted Quail1International Journal of Poultry Science, 2019, 18(6), 284-292.
Comparison of Three Different Vaccination Protocols Against Avian Infectious Anemia with One and Two Vaccines in Breeders and their Impact on ProgenyInternational Journal of Poultry Science, 2023, 22(1), 84-89.
Cited By
Evaluation of germination, distribution, and persistence of Bacillus subtilis spores through the gastrointestinal tract of chickens
Poultry Science, 2014, 93(7), 1793. DOI: 10.3382/ps.2013-03809
Protective effects of a food-grade recombinant Lactobacillus plantarum with surface displayed AMA1 and EtMIC2 proteins of Eimeria tenella in broiler chickensMicrobial Cell Factories, 2020, 19(1), . DOI: 10.1186/s12934-020-1297-4
Coccidiosis: Recent Progress in Host Immunity and Alternatives to Antibiotic StrategiesVaccines, 2022, 10(2), 215. DOI: 10.3390/vaccines10020215