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Research Article
Attenuation of Antheraea mylitta Cytoplasmic Polyhedrosis Virus (AmCPV) and its Potential as an Oral Vaccine Against Virus Diseases in Tasar Silkworm, Antheraea mylitta D

Gajendra Pal Singh, Ajit Kumar Sinha, Kallahally Nagendra Madhusudhan, Phani Kiran Kumar, Bhadwan Chandra Prasad, Deepak Kumar Roy and Alok Sahay

International Journal of Virology, 2011, 7(1), 33-38.

Abstract

The aim of the present investigation was to attenuate the Antheraea mylitta Cytoplasmic Polyhedrosis Virus (AmCPV) and to test their efficacy against the AmCPV infection in tasar silkworm. AmCPV was attenuated by treatment of Na2CO3 and formalin. The attenuated AmCPV were tested for its potential as oral vaccine in tasar silkworm, Antheraea mylitta D. against the infection of cytoplasmic polyhedrosis virus. The vaccination with attenuated AmCPV 24 h prior to challenge inoculation was effective to protect tasar silkworm from infection of cytoplasmic polyhedrosis virus. It was effective at higher dosages of 30 and 40 μL mL-1. Triple vaccination protected silkworm for comparatively a longer period and reduced the mortality 80.78% due to cytoplasmic polyhedrosis virus infection.

ASCI-ID: 45-170

Cited References Fulltext

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