Research Article 
Molecular Variation of Potato virus Y Isolated from Egypt
E.A. El-Absawy, Amal Mahmoud, A.A. Hemeida and M. Helmy
International Journal of Virology, 2012, 8(1), 81-89.
Abstract
Potato Virus Y (PVY) is one of the most important viruses affecting cultivated potatoes in Egypt. Different potato plants were collected from an experimental station in Giza Governorate, Egypt and were tested using RT-PCR. PVY was amplified using primers represented portion of the Coat Protein (CP) gene and 3' Untranslated Regions (UTR). Phylogenetic tree showed two main strain groups: Group I regroups PVYN and PVYNTN stains while Group II includes PVYO, PVYW and PVYN:O strains. The Egyptian PVY isolate was clearly classified within group I and was more closely related to PVYNTN strains. Ten nucleotide substitutions resulted in 3 conserved amino acid substitutions (V1→I, G7→E, M or V and S8→G) and were able to differentiate between both groups. The partial coat protein region was more diverse than that of the 3'UTR (92.6-100% and 97.7-100% identity, respectively). The 3'UTR of the Egyptian isolate showed RNA secondary structures different from those of the 5 PVY strains.
ASCI-ID: 45-197
Similar Articles
Genome Sequencing, Comparison and Phylogenetic Analysis of Citrus yellow mosaic virus Isolates Originating from Different Citrus Species in India
International Journal of Virology, 2009, 5(4), 143-153.
First Report of Coat Protein Sequence of Cucumber Green Mottle Mosaic Virus in Cucumber Isolated from Khorasan in IranInternational Journal of Virology, 2011, 7(1), 1-12.
Nucleotide Sequencing and Phylogenic Analysis of Fusion (F) Epitope for Egyptian Pestes Des Petit Ruminants Virus (PPRV) Predicting Unique Criteria Stated as Egypt 2009International Journal of Virology, 2011, 7(4), 204-209.
Biological, Serological and Molecular Diagnosis of Three Major Potato Viruses in EgyptInternational Journal of Virology, 2009, 5(2), 77-88.
Evaluation of Some Therapies to Eliminate Potato Y Potyvirus from Potato PlantsInternational Journal of Virology, 2009, 5(2), 64-76.
Detection of Three Potato Viruses Isolated from Saudi ArabiaInternational Journal of Virology, 2014, 10(3), 224-234.
Chemotherapy of Potato Virus Y Infecting Potato Plants Using Antiviral DrugsInternational Journal of Virology, 2021, 17(1), 8-19.
Use of Helper Component Proteinase Gene to Identify a New Egyptian Isolate of Watermelon Mosaic PotyvirusInternational Journal of Virology, 2007, 3(3), 107-116.
Cloning and Sequencing of a cDNA Encoding the Coat Protein of an Egyptian Isolate of Pepper mild mottle virusInternational Journal of Virology, 2009, 5(2), 109-118.
Etiology of a Mosaic Disease of Radish and Lettuce and Sequencing of the Coat Protein Gene of the Causal Agent in Saudi ArabiaInternational Journal of Virology, 2009, 5(3), 131-142.
Prevalence of Respiratory Syncytial Virus Infection in Riyadh During the Winter Season 2007-2008 and Different Risk Factors ImpactInternational Journal of Virology, 2009, 5(4), 154-163.
Differentiation Study between Alfalfa Mosaic Virus and Red Clover Mottle Virus Affecting Broad Bean by Biological and Molecular CharacterizationInternational Journal of Virology, 2010, 6(4), 224-239.
Differentiation Among Three Egyptian Isolates of Citrus psorosis virusInternational Journal of Virology, 2009, 5(2), 49-63.
Elimination of Grapevine fanleaf virus (GFLV) and Grapevine leaf roll-associated virus-1 (GLRaV-1) from Infected Grapevine Plants Using Meristem Tip CultureInternational Journal of Virology, 2009, 5(2), 89-99.
Single-Step Multiplex Reverse Transcription-Polymerase Chain Reaction (m-RT-PCR) for Simultaneous Detection of Five RNA Viruses Affecting Stone Fruit TreesInternational Journal of Virology, 2009, 5(2), 100-108.
First Report of Coat Protein Sequence of Cucumber Green Mottle Mosaic Virus in Cucumber Isolated from Khorasan in IranInternational Journal of Virology, 2011, 7(1), 1-12.
Genetic Variations in a Conserved 5'-untranslated Region of Hepatitis C Virus Isolated from EgyptInternational Journal of Virology, 2011, 7(3), 91-99.
Serological and Molecular Identification of Some Isolated Avian Influenza Viruses During Outbreaks in EgyptInternational Journal of Virology, 2011, 7(4), 123-134.
Biological and Molecular Characterization of Potato Infecting Alfalfa Mosaic Virus in EgyptInternational Journal of Virology, 2012, 8(1), 106-113.
Characterization and Elimination of a TMV Isolate Infecting Chrysanthemum Plants in EgyptInternational Journal of Virology, 2012, 8(1), 14-26.
Molecular Characterization of Onion Yellow Dwarf Virus (Garlic Isolate) with Production of Virus-free PlantletsInternational Journal of Virology, 2012, 8(1), 61-70.
Partially Nucleotide Sequence and Secondary Structure of Chrysanthemum stunt viroid Egyptian Isolate from Infected-Chrysanthemum PlantsInternational Journal of Virology, 2012, 8(2), 191-202.
Establishment of Dot-blot Hybridization for Diagnosis of Bovine Ephemeral Fever Virus in EgyptInternational Journal of Virology, 2012, 8(3), 271-278.
A New Egyptian Satellite Strain of Cucumber Mosaic CucumovirusInternational Journal of Virology, 2012, 8(3), 240-257.
Double Infections with Cucumber Mosaic Virus and Plum Pox Virus (Sharka) in Apricot TreesInternational Journal of Virology, 2012, 8(1), 50-60.
Detection of Human Metapneumo Virus among Infants with BronchiolitisInternational Journal of Virology, 2014, 10(1), 37-45.
Intraspecific Diversity of Cucumber mosaic Cucumoviridae in EgyptInternational Journal of Virology, 2014, 10(2), 94-102.
Rapid Detection of Foot and Mouth Disease Virus from Tongue Epithelium of Cattle and Buffaloes in Suez Canal Area, Egypt from 2009-2011International Journal of Virology, 2014, 10(1), 55-62.
Indexing of Potato leaf roll virus (PLRV) from Potato Growing Areas of Punjab, IndiaInternational Journal of Virology, 2014, 10(4), 272-279.
Serological and Molecular Characterization of Cucurbit chlorotic yellows virus Affecting Cucumber Plants in EgyptInternational Journal of Virology, 2015, 11(1), 1-11.
Nucleotide Sequence of Capsid Protein Gene of Bean yellow mosaic potyvirus in Bean Plants from Al-Makhwah Governorate, Saudi ArabiaInternational Journal of Virology, 2015, 11(2), 41-53.
Serological and Molecular Identification of Tobacco streak ilarvirus Infecting Onion (Allium cepa L.) on Commercial Fields in Southern IndiaInternational Journal of Virology, 2016, 12(1), 18-25.
Cited By
Occurrence of Diverse Recombinant Strains of Potato virus Y Circulating in Potato Fields in Egypt
Plant Disease, 2017, 101(8), 1463. DOI: 10.1094/PDIS-02-17-0275-RE
Preliminary characterization of potato virus Y (PVY) populations in Algerian potato fieldsJournal of Plant Pathology, 2018, (), . DOI: 10.1007/s42161-018-0103-1
Biodiversity and full genome sequence of potato viruses Alfalfa mosaic virus and potato leaf roll virus in EgyptZeitschrift für Naturforschung C, 2018, 73(11-12), 423. DOI: 10.1515/znc-2018-0033
Potato virus Y isolates infecting bell pepper from parts of Southern Africa display distinct recombination patternsPhysiological and Molecular Plant Pathology, 2021, 114(), 101638. DOI: 10.1016/j.pmpp.2021.101638
Investigation into the strain typing of Potato Virus Y isolates in EgyptJournal of Plant Diseases and Protection, 2021, 128(2), 369. DOI: 10.1007/s41348-020-00399-7
Potato virus Y recombinant coat protein production in Escherichia coli : Applications in antibody production and virus detectionJournal of Phytopathology, 2021, (), . DOI: 10.1111/jph.12997