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Research Article
Genetic Variation of Limnonectes blythii (Anura: Dicroglossidae) Using RAPD (Random Amplified Polymorphic DNA) Analysis in West Sumatra

Wince Hendri, Djong Hon Tjong, Dahelmi and Dewi Imelda Roesma

International Journal of Zoological Research, 2019, 15(1), 1-5.

Abstract

Background and Objective: Limnonectes blythii population in west Sumatra sharply decline because of its trading activity and damaged habitat. Conservation effort is necessary to protect the species. One of important information that needs to be done is to understand the species genetic variation. The aim of this study was to analyze the genetic variety of Limnonectes blythii of west Sumatra using Random Amplified Polymorphic DNA (RAPD). Materials and Methods: Thirty frogs were collected from three location of wildlife sanctuary in west Sumatra. Genetic variation analysis using RAPD with 11 OPA primer. The electrophoresis results were marked as 1 if a band founded and 0 if there’s no band. Results: Genetic variation of three population were low and the highest founded on Malampah population , next on Sijunjung and lastly Harau . However, genetic differentiation inter-population was considered moderate, which of half of overall genetic variation generated from differentiation inter-population and also a half from differentiation of intra-population which supported by lower heterozygosity inter-population than intra-population heterozygosity. Gen flow value of inter-population was observed moderate. Conclusion: Genetic variation of L. blythii intra-population is lower than inter-population and the gene flow between L. blythii population considered moderate.

ASCI-ID: 46-297

Cited References Fulltext

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