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Research Article
Analysis of Black Seed Effect on Aedes aegyptii

Fahma Wijayanti, Moh Naim and Ibnu Sina

International Journal of Zoological Research, 2019, 15(1), 13-20.


Background and Objective: Insect vectors, especially mosquitoes are responsible for spreading serious human diseases like malaria, Japanese encephalitis, yellow fever, dengue and filariasis. The various synthetic products and devices designed to combat such vectors are not successful because of increased resistance developed by various mosquito species. The usage of plant extracts is one of the possible methods of pollution free method in insect control. The objective of this study was to find new method for controlling adult of Aedes aegyptii using extract seeds of Nigella sativa. Materials and Methods: The study was conducted from July, 2016-August, 2017 at Laboratory of Toxicology, University of Malaya, Kuala Lumpur, Malaysia. The crude extract was obtained by powdered seed of Nigella sativa and extracted by n-hexane solution for 72 h. Bioassays was conducted using both Y-Olfactometer and wind tunnel condition. Identification of Nigella sativa has been conducted by injecting crude extract into GC-MS. Results: Dual choice bioassay showed that 64% females mosquito significantly (p<0.001) avoided black seed within 3.2 min whereas the longest time was 10.29 min for female mosquito to avoid the black seed at concentration 5000 ppm. In the wind tunnel, single mosquito was tested against 250-5000 ppm for 100 cm in wind or no wind conditions. In wind tunnel assays the threshold for single mosquito to exhibit avoidance behavior was 1000 and 5000 ppm in the presence of wind within 100 cm whereas it was 5000 ppm in the presence of no wind condition within 100 cm. The result of this study showed that the compounds of styrol, thymoquinone and p-cymene in the black seed were confirmed to be effective disturbing the behavior of mosquito. Conclusion: The black seed Nigella sativa has a potential to managing behavior of mosquito Aedes agyptii and can be used in term of integrated management system of pest control.

ASCI-ID: 46-300

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Agro-waste derived compounds (flax and black seed peels): Toxicological effect against the West Nile virus vector, Culex pipiens L. with special reference to GC–MS analysis

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