In vitro and in vivo Antibacterial Activity of Three Natural Substances, for the Control of Xanthomonas albilineans Responsible for Sugarcane Leaf Scald in Côte d'Ivoire

Research Article
In vitro and in vivo Antibacterial Activity of Three Natural Substances, for the Control of Xanthomonas albilineans Responsible for Sugarcane Leaf Scald in Côte d'Ivoire

Klinnanga Noël Kone, Konan Didier Kouamé, Aya Carine N'Guessan, Koffi Fernand Jean-Martial Kassi, Kouadio Jacques-Edouard Yao, Barakissa Bamba, Kassoum Kone and Daouda Kone

Journal of Agronomy, 2023, 22(1), 1-12.

Abstract

Background and Objective: Sugarcane cultivation in Côte d'Ivoire is subject to numerous biotic constraints, notably leaf scald caused by Xanthomonas albilineans. The present study was initiated with a view to controlling this disease using natural substances. Materials and Methods: The experiment was carried out in the laboratory. The in vitro efficacy of three essential oils was evaluated on three strains of Xanthomonas albilineans by deposition on YPGA medium. Their efficacy was tested on leaf slices of the R585 sugarcane variety, inoculated by soaking for 3 hrs in the laboratory. An analysis of variance was applied to the recorded data. Results: The essential oils of Ocimum gratissimum L. and the combination of Zingiber officinale+Ocimum gratissimum L. were the most effective in vitro, at concentrations of 4000 and 8000 ppm with diameters greater than 11 mm. Under in vivo conditions and at a concentration of 8000 ppm, the essential oil of Ocimum gratissimum L. (Oci) and the combination of Zingiber officinale+Ocimum gratissimum L. (ZiOci) proved the most effective. Conclusion: The essential oils tested were effective in vitro at concentrations of 4000 and 8000 ppm and in vivo at 6000 and 8000 ppm. These natural substances could provide an alternative to chemical control of sugarcane leaf scald.

ASCI-ID: 31-765

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