Research Article 
Anticandidal and Antistaphylococcal Activity of Soap Fortified with Ocimum gratissimum Extract
A.A. Ogunnowo, C.N. Ezekiel, C.P. Anokwuru, Y.A. Ogunshola and O.O. Kuloyo
Journal of Applied Sciences, 2010, 10(18), 2121-2126.
Abstract
The possibility of producing a highly competitive water-soluble hard soap fortified with O. gratissimum extract (Ts) which will inhibit the growth and activity of Candida and staphylococci was investigated in comparison to a commercial standard soap (Ss) and the control soap without an extract (Cs). The standard agar well diffusion method and broth micro-dilution assay technique were used for the antimicrobial potency determination of the soap samples and Minimal Inhibitory Concentrations (MIC) respectively. The pH range of the soap samples was 9.8-10.2 ±0.2. Ts had clear forest green coloration as compared to the creamy appearance of Ss and Cs. The moisture content (%), foamability (cm) and total fatty matter (%) values for Ts were 19.7±0.02, 13.4±0.03 and (10.0±0.01), respectively. The inhibitory activity of soap fortified with O. gratissimum against all Candida sp. and Staphylococci was significant over Cs but insignificant towards Ss (p<0.05). The sensitivity values (mm) for C. albicans, C. parapsilosis and the staphylococci to Ts ranged between 9.5±0.02-23.1±0.01, 8.7±0.00-21.5±0.02 and 6.5±0.01-26.5±0.02, respectively, as compared to their low sensitivity to Cs, (6.2±0.01-17.0±0.00), (6.5±0.01-15.8±0.00) and (6.2±0.01-18.0±0.00), respectively. The MIC was 0.0312 and 0.0156 g mL-1 for the Ss and Cs and Ts, respectively for all isolates (Candida and staphylococci). On an overall, Ts was highly comparable with Ss as compared to the Cs in terms of general acceptability, texture and fragrance and other analyzed parameters.
ASCI-ID: 35-3627
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