Search. Read. Cite.

Easy to search. Easy to read. Easy to cite with credible sources.

Research Article
Alleviation of Inhibitory Effects of Staphylococcus aureus on MPO Expression and Myeloperoxidase Activity in Human Neutrophils in vitro by Piper betle Linn. Ethanolic Extract

Yalda Modirrahmati, Mohd Fahmi Mastuki and Roslinah Mohamad Hussain

Journal of Applied Sciences, 2015, 15(6), 874-883.

Abstract

Dissemination of S. aureus within the host is facilitated by its ability to survive neutrophils (PMNs) clearance mechanisms. Identifying agents from natural sources that can boost neutrophil killing ability is therefore beneficial. We investigated effects of P. betle ethanolic extract on Myeloperoxidase (MPO) and killing potential of PMNs. Staphylococcus aureus suppresses MPO expression in PMNs with corresponding loss of MPO activity and 19.23% protein reduction. Piper betle extract alleviated this repressor effect with threefold (3.106 mU mL-1 μg-1 protein) increase in specific MPO activity in 30 min and 13.44% increase in protein concentration PMNs treated with S. aureus together with extract increased activity even higher (3.629 mU mL-1 μg-1 protein) with a corresponding 19.23% increase in protein concentration. The RT-qPCR analyses confirmed that S. aureus down-regulates MPO causing reduced expression (0.053 fold), whereas, treating PMNs with S. aureus with extract increased MPO expression by 2.29 fold. Staphylococcus aureus showed 79.24% survival in PMNs, whereas, addition of extract enhanced neutrophil killing ability as reflected by a marked reduction in cell survival to 7.14%. Piper betle ethanolic extract overcomes the inhibitory effects of Staphylococcus aureus on myeloperoxidase, resulting in increased clearance of intracellular cells.

ASCI-ID: 35-6321

Cited References Fulltext

Related Articles


Anticandidal and Antistaphylococcal Activity of Soap Fortified with Ocimum gratissimum Extract

Journal of Applied Sciences, 2010, 10(18), 2121-2126.

Screening Antimicrobial Activity of Venoms from Snakes Commonly Found in Malaysia

Journal of Applied Sciences, 2010, 10(19), 2328-2332.

Plasma Sterilization Using the High Voltage Pulsed Discharge at Atmospheric Pressure

Journal of Applied Sciences, 2006, 6(7), 1566-1570.

Antibacterial Activity of the Essential Oils of Sudanese Accessions of Basil (Ocimum basilicum L.)

Journal of Applied Sciences, 2009, 9(23), 4161-4167.

Cited By


Aronia melanocarpa Elliot anthocyanins inhibit colon cancer by regulating glutamine metabolism

Food Bioscience, 2021, 40(), 100910. DOI: 10.1016/j.fbio.2021.100910

Microbial lipases: Propitious biocatalysts for the food industry

Food Bioscience, 2022, 45(), 101509. DOI: 10.1016/j.fbio.2021.101509

The combined effect of ultrasound treatment and leek (Allium ampeloprasum) extract on the quality properties of beef

Food Bioscience, 2022, 47(), 101622. DOI: 10.1016/j.fbio.2022.101622

An adaptive clustering algorithm by neighbourhood search for large-scale data

Journal of Statistical Computation and Simulation, 2022, (), 1. DOI: 10.1080/00949655.2022.2098298

Genoprotection and metabolic benefits of marine macroalgae - Insights into the concept of functional foods through direct and indirect consumption

Food Bioscience, 2022, 47(), 101649. DOI: 10.1016/j.fbio.2022.101649

Distribution of blood groups in the Iranian general population

Immunohematology, 2016, 32(4), 135. DOI: 10.21307/immunohematology-2019-058

Valorization of fruit wastes for circular bioeconomy: Current advances, challenges, and opportunities

Bioresource Technology, 2022, 359(), 127459. DOI: 10.1016/j.biortech.2022.127459

Advances in fermentative production, purification, characterization and applications of gellan gum

Bioresource Technology, 2022, 359(), 127498. DOI: 10.1016/j.biortech.2022.127498

Enterobacter asburiae KUNi5, a Nickel Resistant Bacterium for Possible Bioremediation of Nickel Contaminated Sites

Polish Journal of Microbiology, 2016, 65(1), 115. DOI: 10.5604/17331331.1197284

Prevalence of Parasitic Contamination in Salad Vegetables Collected from Supermarkets and Street Vendors in Amman and Baqa’a – Jordan

Polish Journal of Microbiology, 2016, 65(2), 201. DOI: 10.5604/17331331.1204480

Suppressive Effect of Trichoderma spp. on toxigenic Fusarium species

Polish Journal of Microbiology, 2017, 66(1), 85. DOI: 10.5604/17331331.1234997

In vitro Interaction between Fumonisin B1 and the Intestinal Microflora of Pigs

Polish Journal of Microbiology, 2017, 66(2), 245. DOI: 10.5604/01.3001.0010.7858

Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa in a Healthcare Setting in Alexandria, Egypt

Polish Journal of Microbiology, 2017, 66(3), 297. DOI: 10.5604/01.3001.0010.4855

Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity

Polish Journal of Microbiology, 2018, 67(4), 441. DOI: 10.21307/pjm-2018-052

Bioactive Compounds of Pseudoalteromonas sp. IBRL PD4.8 Inhibit Growth of Fouling Bacteria and Attenuate Biofilms of Vibrio alginolyticus FB3

Polish Journal of Microbiology, 2019, 68(1), 21. DOI: 10.21307/pjm-2019-003

An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

Polish Journal of Microbiology, 2019, 68(2), 193. DOI: 10.33073/pjm-2019-019

Inhibition of Drug Resistance of Staphylococcus aureus by Efflux Pump Inhibitor and Autolysis Inducer to Strengthen the Antibacterial Activity of β-lactam Drugs

Polish Journal of Microbiology, 2019, 68(4), 477. DOI: 10.33073/pjm-2019-047

Effect of Chlorella vulgaris on Growth and Photosynthetic Pigment Content in Swiss Chard (Beta vulgaris L. subsp. cicla)

Polish Journal of Microbiology, 2020, 69(2), 235. DOI: 10.33073/pjm-2020-023

Complex coacervation microcapsules by tannic acid crosslinking prolong the antifungal activity of cinnamaldehyde against Aspergillus brasiliensis

Food Bioscience, 2022, 47(), 101686. DOI: 10.1016/j.fbio.2022.101686

Luffa cylindrica Immobilized with Aspergillus terreus QMS-1: an Efficient and Cost-Effective Strategy for the Removal of Congo Red using Stirred Tank Reactor

Polish Journal of Microbiology, 2020, 69(2), 193. DOI: 10.33073/pjm-2020-022

Development of gluten-free tarhana formulations: Part II. Utilization of legume composite flours and baker’s yeast

Food Bioscience, 2022, 47(), 101692. DOI: 10.1016/j.fbio.2022.101692

Improved Biosurfactant Production by Enterobacter cloacae B14, Stability Studies, and its Antimicrobial Activity

Polish Journal of Microbiology, 2020, 69(3), 273. DOI: 10.33073/pjm-2020-030