Research Article 
Resveratrol Inhibits Invasion and Metastasis of Hepatocellular Carcinoma Cells
Hai-Bo Yu, De-Yu Li, Hui-Feng Zhang, Huan-Zhou Xue, Cheng-En Pan, Si-Hai Zhao and Lei Wang
Journal of Animal and Veterinary Advances, 2010, 9(24), 3117-3124.
Abstract
Resveratrol (trans-3, 4', 5-trihydroxystilbene) is a natural phytoalexin found in grapes and other plants that has anti-cancer and anti-inflammatory effects. To observe its effects of on proliferation of human hepatocellular carcinoma cells and the underlying molecular mechanisms, we performed the current study. MTT assay was used to assess the effects of resveratrol on proliferation of human hepatocellular carcinoma cell line HepG2. Flow cytometry and invasion assays were performed to detect apoptosis and tumor cell invasion capacity was detected in vitro and in vivo. After that Real-time quantitative PCR and western blotting assays were used to detect the expression of MMP-9 mRNA and protein, respectively. MMP-9 activity was also analyzed by gelatin zymography assay. NF-kappa B was measured by electrophoretic mobility shift assay. Resveratrol inhibited growth of HepG2 cells with a dose and time-dependent manner. The reduced cell viability is due to apoptosis or proliferation inhibited by resveratrol. Invasion assay found that resveratrol induce a decrease in the potential for invasion. Molecularly, resveratrol was able to reduce both MMP-9 mRNA expression and protein levels as wellas MMP-9 enzymatic activity which may be via the inhibition of NF-kappaB activity. The data showed that resveratrol was able to reduce viability of hepatocellular carcinoma cells which is due to induction of apoptosis by resveratrol. Resveratrol also inhibited tumor cell invasion capacity which is through down regulation of MMP-9 levels and its enzymatic activity. Future study will investigate the effects of resveratrol in vivo in control of hepatocellular carcinoma cell in clinics.
ASCI-ID: 156-2523
Similar Articles
Meiotic Competence and DNA Damage of Porcine Oocytes from Ovaries Exposed to an Elevated Temperature
Journal of Animal and Veterinary Advances, 2008, 7(10), 1179-1183.
Fas-Associated Death Domain (FADD) Mediated Activation of a Apoptosis Program in Bovine Follicular Granulosa CellsJournal of Animal and Veterinary Advances, 2011, 10(15), 1994-2003.
The Apoptotic Effects of Alcohol Shock on Bovine OocytesJournal of Animal and Veterinary Advances, 2011, 10(20), 2679-2682.
Effect of Pentoxifylline on Renal Ischemic ReperfusionJournal of Animal and Veterinary Advances, 2011, 10(20), 2675-2678.
Increasing of Caspase-1 mRNA in the Lung and Heart of Hyperthyroid Broiler ChickensJournal of Animal and Veterinary Advances, 2011, 10(22), 3010-3013.
Protective Effect of Metformin on Cardiomyocytes Ischemia-Reperfusion (IR) Induced Apoptosis in RatsJournal of Animal and Veterinary Advances, 2012, 11(1), 108-112.
Effect of Simvastatin on Apoptosis Inhibition in Renal Tissue after Unilateral Ureteral Obstruction in RatJournal of Animal and Veterinary Advances, 2011, 10(23), 3129-3133.
Effect of PPARα’s over Expression on the Type 2 Diabetic Rat CardiomyocytesJournal of Animal and Veterinary Advances, 2012, 11(10), 1588-1594.
The Effects of Resveratrol Supplementation on Exercise-Induced Apoptosis of Rat HepatocytesJournal of Animal and Veterinary Advances, 2012, 11(11), 1921-1924.
IGF-1 Protects Myocardial Cells from ROS Stress-Induced Apoptosis via Up-Regulating ARCJournal of Animal and Veterinary Advances, 2012, 11(11), 1901-1906.
Amaryllidaceae Alkaloids Exhibit Anti-Influenza Activity in MDCK Cells, an Investigation of Amaryllidaceae Alkaloids and MDCK Cells InsightJournal of Animal and Veterinary Advances, 2012, 11(14), 2485-2492.
Effects of Grape Seed Extract on Renal Ischemic Reperfusion in RatsJournal of Animal and Veterinary Advances, 2012, 11(14), 2529-2532.
The Effects of MEM Vitamins and β-Mercaptoethanol on Porcine Embryonic Development, Reactive Oxygen Species and ApoptosisJournal of Animal and Veterinary Advances, 2012, 11(14), 2576-2583.
Histological, Histochemical and Immunohistochemical Investigations on the Developing Small Intestines of Broiler EmbryosJournal of Animal and Veterinary Advances, 2012, 11(16), 2936-2944.
Detection of Apoptosis in Experimental Fibrosarcoma Using DNA Fragmentation and Immunohystochemical MethodsJournal of Animal and Veterinary Advances, 2012, 11(17), 3054-3058.
The Effect of Helicobacter pylori on Apoptosis of the GES-1 Transfected pEGFP-N1-UreB Eukaryotic Expression Plasmid in vitroJournal of Animal and Veterinary Advances, 2012, 11(23), 4435-4440.
Protective Effect of Kinetin on Iso-Induced Myocardial Ischemia in vitro and in vivoJournal of Animal and Veterinary Advances, 2012, 11(24), 4617-4624.
Effects of Dietary Vitamin D3 on the Apoptosis, Morphological Structure and Antibacterial Peptide Hepcidin Gene Expression in the Spleen of Monopterus albusJournal of Animal and Veterinary Advances, 2013, 12(3), 316-322.
Effects of Resveratrol on Testis Damage in Streptozotocin Induced Diabetic RatsJournal of Animal and Veterinary Advances, 2013, 12(6), 747-753.
Oxidative Stress Induced by Diabetes Activates Apoptosis in Rat BrainJournal of Animal and Veterinary Advances, 2013, 12(9), 927-931.
Apoptosis in the Bovine Oocytes by Different in vitro Culture ConditionsJournal of Animal and Veterinary Advances, 2014, 13(9), 570-576.
Resveratrol Inhibits MMP-2 Expression of Hepatoma in Nude MiceJournal of Animal and Veterinary Advances, 2011, 10(1), 33-37.
Expression of Augmenter of Liver Regeneration and Nuclear Faction-κB in Human Hepatocellular CarcinomaJournal of Animal and Veterinary Advances, 2011, 10(4), 480-483.
Range Expansion of Introduced Tilapia Species (Oreochromis niloticus, L. 1758, Cichlidae) in TurkeyJournal of Animal and Veterinary Advances, 2010, 9(12), 1753-1756.
Erythrocyte Sizes of Carassius gibelio Species in Porsuk Dam Lake (Eskisehir/Turkey)Journal of Animal and Veterinary Advances, 2010, 9(24), 3077-3082.
Resveratrol Inhibits MMP-2 Expression of Hepatoma in Nude MiceJournal of Animal and Veterinary Advances, 2011, 10(1), 33-37.
Study on Mechanism for P21/53 Promoting Invasion of Human Glioma Stem Cells by Inhibiting TIMP-2 ExpressionJournal of Animal and Veterinary Advances, 2013, 12(14), 1220-1227.
Resveratrol Inhibits MMP-2 Expression of Hepatoma in Nude MiceJournal of Animal and Veterinary Advances, 2011, 10(1), 33-37.
The Effects of Resveratrol Supplementation on Exercise-Induced Apoptosis of Rat HepatocytesJournal of Animal and Veterinary Advances, 2012, 11(11), 1921-1924.
Effect of Resveratrol on the Expression of p38 Mitogen Activated Protein Kinase and Intercellular Adhesion Molecule-1 in Rats with Lipopolysaccharide-Induced Acute Lung InjuryJournal of Animal and Veterinary Advances, 2012, 11(11), 1831-1836.
Immunohistochemical Study of the Effect of Resveratrol on the Expression of β-Catenin Protein in Experimental Colonic Carcinoma of RatJournal of Animal and Veterinary Advances, 2012, 11(23), 4472-4475.
Effects of Resveratrol on Testis Damage in Streptozotocin Induced Diabetic RatsJournal of Animal and Veterinary Advances, 2013, 12(6), 747-753.