Research Article 
A Rapid and Cost Effective Method for Genotyping Two Types of GHR Mutations in Sex-Linked Dwarf Chicken
Yan Wang, Hao Qu, Cheng-Long Luo, Jie Wang and Ding-Ming Shu
Journal of Animal and Veterinary Advances, 2012, 11(11), 1873-1875.
Abstract
Large deletion or mutation at the exon 10 and 3' untranslated region of Growth Factor Receptor (GHR) gene results in dwarf chicken and the common method for detecting mutation is direct PCR amplification. But since, the target fragment is large, the amplification is inefficient and unreliable and this process consumes a lot of expensive specific DNA polymerases. Researchers focused on the point mutation C146280A in the deletion fragment and set up a new method to detect large deletion and point mutation in this region. Comparing with direct PCR this new method which can detect both large deletion and point mutation with regular low-cost DNA polymerases is more stable, reliably and cheapness.
ASCI-ID: 156-3477
Similar Articles
Genotypic Comparison of Some Indian Chicken Populations with East Lansing Reference Populations
Journal of Animal and Veterinary Advances, 2007, 6(1), 49-52.
Genotyping of Staphylococcus aureus Isolated from Bovine Clinical Mastitis by Pulsed-Field Gel Electrophoresis (PFGE)Journal of Animal and Veterinary Advances, 2010, 9(1), 5-11.
The Analysis of ERIC-PCR Genomic Polymorphism of Salmonella Isolated Strains in Pig CarcassJournal of Animal and Veterinary Advances, 2011, 10(13), 1694-1698.
The Research of KRG Serotyping and ERIC-PCR and PCR-RFLP Genotyping of Haemophilus parasuis Isolates from Anhui Province of ChinaJournal of Animal and Veterinary Advances, 2011, 10(20), 2669-2674.
Association of Growth Traits with SSCP Polymorphisms at the Growth Hormone Receptor (GHR) and Growth Hormone Releasing Hormone Receptor (GHRHR) Genes in the Baluchi SheepJournal of Animal and Veterinary Advances, 2009, 8(6), 1063-1069.
Mutation in Exon 4 of apoVLDL-II Gene is a Candidate for Meat Tenderness in ChickenJournal of Animal and Veterinary Advances, 2008, 7(12), 1624-1627.
MEF2A Gene Polymorphisms are Associated with Growth Traits in Chinese Indigenous Cattle BreedsJournal of Animal and Veterinary Advances, 2010, 9(4), 814-819.
Polymorphic Study of FecXG, FecGH and FecB Mutations in Four Domestic Sheep Breeds in the Lower Yellow River Valley of ChinaJournal of Animal and Veterinary Advances, 2011, 10(17), 2198-2201.
Polymorphism Analysis on the Second Intron of the GH Gene in Chinese DonkeysJournal of Animal and Veterinary Advances, 2012, 11(7), 916-918.
MSTN Gene Polymorphism and its Relationships with Growth Performance in Ma Shen PorcineJournal of Animal and Veterinary Advances, 2012, 11(8), 1173-1176.
Geometrical Study on FMDV Genome Based on Z-CurveJournal of Animal and Veterinary Advances, 2012, 11(15), 2630-2640.
The Correlation Between Goat Cox II Gene Single Nucleotide Polymorphism and Sperm MotilityJournal of Animal and Veterinary Advances, 2012, 11(20), 3677-3681.