Research Article 
Establishment and Biological Characteristics of Qing Kedan Chicken Embryonic Fibroblast Line for Genetic Resource Conservation
Hui Wang, Xiangchen Li, Changli Li, Wenxiu Zhang, Weijun Guan and Yuehui Ma
Journal of Animal and Veterinary Advances, 2012, 11(23), 4374-4381.
Abstract
A fibroblast cell line of Qing Kedan chicken was successfully isolated, purified and cryopreseved through direct culturing of explants, serial passage and cryogenic techniques. Assays for biological characteristics were conducted and the results suggested that: the cells cultured with a Population Doubling Time (PDT) of about 20 h, constituted a typical fibroblast cell line in morphology; tests for microbial contamination regarding bacteria, fungi, viruses and mycoplasmas were exclusively negative; Lactate Dehydrogenase (LDH) and Malate Dehydrogenase (MDH) polymorphism analysis disproved the existence of cross-contamination from other cell lines; diploid cells making up 85-93% of the population verified the hereditary stability of the fibroblast line. pEGFP-N3, pEYFP-N1 and pDsRed1-N1 were transfected into the Qing Kedan chicken embryonic fibroblasts with transfection efficiencies between 12.6 and 39.7%. To locate their cellular distribution, transfected cells were observed at 24, 48 and 72 h with confocal microscopy and the fluorescences could be observed throughout the cytoplasm and nuclei of positive cells except in cryptomere vesicles. This research preserved the precious germplasm resource of Qing Kedan chicken at cell level and hence served as an enlightening reference for those of other poultry in the world.
ASCI-ID: 156-3937
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Detection Class I Integron of Multidrug Resistace Pathogenic Salmonella from Chicken in Hebei Province, ChinaJournal of Animal and Veterinary Advances, 2012, 11(6), 742-745.
Detection of the Major Macrolide Resistance Genes in Streptococcus suis Serotype 2 Isolates in Hebei Province ChinaJournal of Animal and Veterinary Advances, 2012, 11(6), 781-784.
Seroprevalence of Toxoplasma gondii and Neospora caninum in Tarim Red Deer (Cervus elaphus yarkandensis) from Xinjiang Province, Northwest ChinaJournal of Animal and Veterinary Advances, 2012, 11(7), 912-915.
Energy and Nitrogen Provided by Native Herbage to Sheep Grazing in Alpine Grasslands of the Qinghai-Tibetan PlateauJournal of Animal and Veterinary Advances, 2012, 11(7), 919-923.
Tachyplesin I Induce Drug Resistance in Bacteria in vitroJournal of Animal and Veterinary Advances, 2012, 11(7), 939-945.
Molecular Cloning and Tissue Expression of Cell Division Cycle 42 in Buffalo TissueJournal of Animal and Veterinary Advances, 2012, 11(7), 1018-1022.
Prokaryotic Expression and Sequence Analysis of Porcine BCL10 GeneJournal of Animal and Veterinary Advances, 2012, 11(7), 1046-1051.
Phylogenetic Analysis of Complete rRNA Gene Sequence of Endoreticulatus sp. Shengzhou from the Silkworm, Bombyx mori in Zhejiang of ChinaJournal of Animal and Veterinary Advances, 2012, 11(7), 1056-1062.
Prevalence Investigation of Dermatophytes in Rabbits in Qingdao Region, ChinaJournal of Animal and Veterinary Advances, 2012, 11(7), 883-885.
Polymorphism of BMP2 Gene Associated with Growth Traits in Guizhou Semi-Fine Wool SheepJournal of Animal and Veterinary Advances, 2012, 11(8), 1110-1115.
Influence and Strategies of Economic Crisis on China’s Livestock FarmingJournal of Animal and Veterinary Advances, 2012, 11(8), 1096-1099.
Plasmid Mediated Streptomycin and Sulfonamide Resistance in Haemophilus parasuisJournal of Animal and Veterinary Advances, 2012, 11(8), 1106-1109.
Pharmacokinetics of Amikacin after Intravenous Intramuscular and Subcutaneous Administration in German Shepherd DogJournal of Animal and Veterinary Advances, 2012, 11(8), 1145-1148.
Molecullar Typing, Prevalence of netB and Antimicrobial Susceptibility among Clinical Isolates of Clostridium perfringens from Chickens in Southern ChinaJournal of Animal and Veterinary Advances, 2012, 11(8), 1183-1187.
Establishment of Two Types of Mammary Epithelial Cell Lines from Chinese Holstein Dairy CowJournal of Animal and Veterinary Advances, 2012, 11(8), 1166-1172.
Characterization and Isolation of Ovine Spermatogonial Stem CellsJournal of Animal and Veterinary Advances, 2012, 11(8), 1242-1245.
Porcine RPL28 Gene, Differentially Expressed in the Backfat Tissue Between Meishan, Large White Pigs and Their F1 HybridsJournal of Animal and Veterinary Advances, 2012, 11(8), 1237-1241.
Identification, mRNA Expression and Characterization of a Novel CENPP Gene from Chinese Banna Mini-Pig Inbred Line (BMI)Journal of Animal and Veterinary Advances, 2012, 11(8), 1230-1236.
Liver Cells of GFP Transgenic Male Mice Transplantation to Reconstruct Hematopoietic Function in Female MiceJournal of Animal and Veterinary Advances, 2012, 11(8), 1258-1264.
Activities of Aspartate Aminotransferase, Alanine Aminotransferase, Gamma-Glutamyltransferase, Alkaline Phosphatase in Plasma of Postpartum Holstein CowsJournal of Animal and Veterinary Advances, 2012, 11(8), 1270-1274.
Molecular Cloning, mRNA Expression and Characterization of a Novel FAIM1 Gene from Chinese Banna Mini-Pig Inbred Line (BMI)Journal of Animal and Veterinary Advances, 2012, 11(8), 1080-1086.
Prevalence and Major Pathogen Causes of Dairy Cows Subclinical Mastitis in Northeast ChinaJournal of Animal and Veterinary Advances, 2012, 11(8), 1278-1280.
Effects of Sijunzi Decoction on Small Intestinal T Lymphocyte Subsets Differentiation in Reserpine Induced Spleen Deficiency RatsJournal of Animal and Veterinary Advances, 2012, 11(9), 1290-1298.
Influence of Salinity and Temperature on the Germination of Haloxylon ammodendron and Ceratoides arborescensJournal of Animal and Veterinary Advances, 2012, 11(9), 1306-1311.
Tissue Expression Level Analysis of CD14 in BuffaloJournal of Animal and Veterinary Advances, 2012, 11(9), 1353-1355.
Study of Enzymolysis Kinetics with Four Kinds of Protein Feeds in Megalobrama amblycephala in vitroJournal of Animal and Veterinary Advances, 2012, 11(9), 1378-1384.
Effects of Dietary Pharmacological Zinc on Growth, Liver Metallothionein, Cu, Zn-SOD Concentration and Serum Parameters in PigletsJournal of Animal and Veterinary Advances, 2012, 11(9), 1390-1394.
Effect of Complex Lactic Acid Bacteria on Silage Quality and In vitro Dry Matter Digestibility of Corn StrawJournal of Animal and Veterinary Advances, 2012, 11(9), 1395-1399.
The MHC class I of Grass Carp (Ctenopharyngodon idellus) With Different Way to Present PeptideJournal of Animal and Veterinary Advances, 2012, 11(9), 1455-1460.
Effects of Dietary Glutamine Supplementation on Nutrient Absorption and Activity of Enzymes Involved in Glutamine Metabolism and Energy Production in the Jejunum of Weaned PigletsJournal of Animal and Veterinary Advances, 2012, 11(9), 1441-1449.
Immunogenicity of Foot and Mouth Disease Virus Type Asia 1 Protein VP1-2A Fused with a Multi-Epitope Expressed in Pichia pastorisJournal of Animal and Veterinary Advances, 2012, 11(9), 1512-1517.
Nutrient and Poisonous Composition in the Mixed Silage of Maize and Astragalus adsurgens Pall. with Varied ProportionsJournal of Animal and Veterinary Advances, 2012, 11(10), 1532-1537.
Different Duration of Cold Stress Enhances Pro-Inflammatory Cytokines Profile and Alterations of Th1 and Th2 Type Cytokines Secretion in Serum of Wistar RatsJournal of Animal and Veterinary Advances, 2012, 11(10), 1538-1545.
Protective Effects of Crude Polysaccharide from Gynostemma pentaphyllum on Swimming Exercise-Induced Oxidative Stress in RatJournal of Animal and Veterinary Advances, 2012, 11(10), 1627-1632.
Molecular Cloning, Sequence Characterization and Tissue Transcription Profile of a Novel Gene PQBP1 from Chinese Banna Mini-Pig Inbred Line (BMI)Journal of Animal and Veterinary Advances, 2012, 11(10), 1674-1680.
Polymorphism Analysis on Intron 3 of the GH Gene in Chinese DonkeyJournal of Animal and Veterinary Advances, 2012, 11(10), 1705-1707.
Effect of High-Cholesterol Diet on Serum Leptin and Blood Lipid in RabbitsJournal of Animal and Veterinary Advances, 2012, 11(10), 1719-1721.
Effects of Radix puerariae (Ge Gen) Water Extract on Exercise-Induced Fatigue in MiceJournal of Animal and Veterinary Advances, 2012, 11(10), 1722-1726.
Identification, Cloning, Expression of a Novel Functional Anas platyrhynchos mRNA TLR4Journal of Animal and Veterinary Advances, 2012, 11(10), 1727-1733.
Bioinformatics Analysis of Melanocortin-1 Receptor Gene for Silver Fox and Other SpeciesJournal of Animal and Veterinary Advances, 2012, 11(7), 1000-1006.
Identification of Intrauterine Mycoplasma in Endometritis of Dairy Cow Isolates by 16S rDNA Nested PCR-DGGEJournal of Animal and Veterinary Advances, 2012, 11(10), 1745-1752.
Effects of Yeast Polysaccharide on Immune Enhancement and Production Performance of RatsJournal of Animal and Veterinary Advances, 2012, 11(11), 1800-1804.
Establishment and Biological Characteristics of Hereford Cattle Fibroblast BankJournal of Animal and Veterinary Advances, 2012, 11(11), 1763-1770.
Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Visual Detection of Porcine Epidemic Diarrhea VirusJournal of Animal and Veterinary Advances, 2012, 11(11), 1897-1900.
Promoter Characterization and Expression Pattern Analysis of Porcine TCAP GeneJournal of Animal and Veterinary Advances, 2012, 11(11), 1876-1880.
IGF-1 Protects Myocardial Cells from ROS Stress-Induced Apoptosis via Up-Regulating ARCJournal of Animal and Veterinary Advances, 2012, 11(11), 1901-1906.
Trichostatin A Treatment on Two Types of Donor Cells for Somatic Cell Nuclear TransferJournal of Animal and Veterinary Advances, 2012, 11(11), 1812-1818.
Determination of Digestive Enzyme Activity in the Digesta from the Small Intestinal of Growing Pigs and Development of in vitro Evaluation System for Feed Bioavailability Using Artificial Small Intestinal JuiceJournal of Animal and Veterinary Advances, 2012, 11(11), 1819-1826.
Effect of Resveratrol on the Expression of p38 Mitogen Activated Protein Kinase and Intercellular Adhesion Molecule-1 in Rats with Lipopolysaccharide-Induced Acute Lung InjuryJournal of Animal and Veterinary Advances, 2012, 11(11), 1831-1836.
Genetic Diversity of Silurus asotus on Mitochondrial DNA 16SrRNA Gene Partial SequenceJournal of Animal and Veterinary Advances, 2012, 11(11), 1843-1846.
Study of a Simple and Rapid PCR Sex Identification of Bovine EmbryoJournal of Animal and Veterinary Advances, 2012, 11(11), 1847-1852.
Transgenic Expression and Antimicrobial Potential of CAMA-Syn in A549 CellsJournal of Animal and Veterinary Advances, 2012, 11(12), 2006-2012.
Advances in Animal Fatty Acid Transmembrane Transport Proteins FATP1 and FATP4Journal of Animal and Veterinary Advances, 2012, 11(12), 2064-2069.
Molecular Cloning and Characterization of a Novel Gene STXBP6 from Chinese Banna Mini-Pig Inbred Line (BMI)Journal of Animal and Veterinary Advances, 2012, 11(12), 2048-2053.
Construction of Full-Length Goose Muscle cDNA LibraryJournal of Animal and Veterinary Advances, 2012, 11(12), 2106-2109.
Expression of Etv4 mRNA in Cryptorchidism Mouse Testis and EpididymisJournal of Animal and Veterinary Advances, 2012, 11(12), 2163-2165.
Effect of Growth Performance and N-Balance of Growing Raccoon Dogs Fed Reduced Crude Protein, Lysine and DL-Methionine Supplementation DietsJournal of Animal and Veterinary Advances, 2012, 11(13), 2187-2190.
Mast Cell Dynamics in the Digestive System During Infection by Muscovy Duck ReovirusJournal of Animal and Veterinary Advances, 2012, 11(13), 2204-2210.
Simple and Rapid Purification of Infectious Laryngotracheitis Virus DNA from Infected TissueJournal of Animal and Veterinary Advances, 2012, 11(13), 2293-2300.
Effect of Enterococcus faecium 1 (EF1) on Antioxidant Functioning Activity of Caco-2 Cells under Oxidative StressJournal of Animal and Veterinary Advances, 2012, 11(13), 2307-2312.
Construction and Characterization of an Expression Plasmid Containing Peste des Petits Ruminants Virus Phosphoprotein GeneJournal of Animal and Veterinary Advances, 2012, 11(13), 2316-2322.
Effects of Fasting Duration and Body Weight on Fasting Heat Production in Growing PigsJournal of Animal and Veterinary Advances, 2012, 11(13), 2333-2341.
Effect of Iron Supplement on the Activity of Catalase, SDH and Their MRNA Expression in Rex RabbitJournal of Animal and Veterinary Advances, 2012, 11(13), 2347-2351.
Several Indicators of Immunity and Antioxidant Activities Improved in Grass Carp Given a Diet Containing Bacillus AdditiveJournal of Animal and Veterinary Advances, 2012, 11(14), 2392-2397.
Contribution of Insertion Within acrR to acrAB Expression and Ciprofloxacin Resistance in a Veterinary Escherichia coli IsolateJournal of Animal and Veterinary Advances, 2012, 11(14), 2436-2439.
Genomic Analysis of One Chinese H1N1 Swine Influenza Virus Strain from Healthy Pig Remaining Different Virulence DeterminantsJournal of Animal and Veterinary Advances, 2012, 11(14), 2465-2468.
Spatiotemporal Dynamincs and Concentrations of Intracellular Free-Ca2+ in in vitro Matured and in vitro Fertilized Bovine Oocytes and Very Early Embryo StagesJournal of Animal and Veterinary Advances, 2012, 11(14), 2516-2522.
Anatomic Peculiarities of the Cerebral Arterial System and Blood Supply in the Yak (Bos grunniens)Journal of Animal and Veterinary Advances, 2012, 11(14), 2533-2539.
Dietary Supplementation with Recombinant Lactoferrampin-Lactoferricin Improves Growth Performance and Affects Serum Parameters in PigletsJournal of Animal and Veterinary Advances, 2012, 11(14), 2548-2555.
The Effects of Rosiglitazone on Fatty Liver of High Fat and Cholesterol-Fed RabbitsJournal of Animal and Veterinary Advances, 2012, 11(14), 2564-2569.
Inflammatory Response of Peripheral Blood Mononuclear Cells Post Intramammary Challenge with Staphylococcus aureus in Dairy CowsJournal of Animal and Veterinary Advances, 2012, 11(14), 2588-2593.
Synthesis and Identification of Aflatoxin B2a Artificial Antigens: The First Critical Step in Preparing Monoclonal Antibodies Against Aflatoxin B1Journal of Animal and Veterinary Advances, 2012, 11(14), 2600-2604.
Application of the Dual-Luciferase Reporter Assay to the Analysis of Mouse Kit and Yy1 Promoter Activation Regulation by Sohlh1Journal of Animal and Veterinary Advances, 2012, 11(14), 2523-2528.
Angiogenic and Osteogenic Responses Related to Femoral Neck Fractures Treated at Different Fracture-To-Surgery Intervals in a Canine ModelJournal of Animal and Veterinary Advances, 2012, 11(15), 2611-2617.
Geometrical Study on FMDV Genome Based on Z-CurveJournal of Animal and Veterinary Advances, 2012, 11(15), 2630-2640.
Impacts of Chicken Manure and Irrigation on Base Cation Input-Output Budgets in a Vegetable Production SystemJournal of Animal and Veterinary Advances, 2012, 11(15), 2652-2659.
Expression of ETS Transcription Factor Etv4 in Busulfan-Treated Mouse Testis and EpididymisJournal of Animal and Veterinary Advances, 2012, 11(15), 2801-2803.
Effects of Tao-Hong-Si-Wu-Tang, A Traditional Chinese Herbal Medicine Formula on Glucose and Lipid Metabolism During Endurance Exercise in Male RatsJournal of Animal and Veterinary Advances, 2012, 11(15), 2737-2740.
Comparative Immunomic Analysis of Schistosoma japonicum Soluble Egg Antigens Reacting with Patient Sera Before and after Praziquantel TreatmentJournal of Animal and Veterinary Advances, 2012, 11(15), 2828-2838.
Sex Determination in Ovine Embryos Using Amelogenin (AMEL) Gene by High Resolution Melting Curve AnalysisJournal of Animal and Veterinary Advances, 2012, 11(16), 2948-2952.
Aerobic Bacterial Flora of Nasal Cavity of Seven Giant Pandas (Ailuropoda melanoleuca)Journal of Animal and Veterinary Advances, 2012, 11(16), 3008-3010.
The Expression Analysis of Ghrelin and Growth Hormone Secretagogue Receptor in Mouse Thymic Epithelial CellsJournal of Animal and Veterinary Advances, 2012, 11(16), 3027-3030.
Tissue Distribution and Relative Quantitation of Experimental Infection with Peste Des Petits Ruminant Virus in Goats Using Real-Time PCR (TaqMan®)Journal of Animal and Veterinary Advances, 2012, 11(16), 3011-3018.
Evaluation of the Suitability of Five Housekeeping Genes as Internal Control for Quantitative Real-Time RT-PCR Assays in Chickens Acutely Infected with IBV M41 StrainJournal of Animal and Veterinary Advances, 2012, 11(17), 3064-3067.
A Human Case Report of Diphyllobothrium latum at Shanghai, ChinaJournal of Animal and Veterinary Advances, 2012, 11(17), 3073-3075.
Serological Surveillance of Swine Influenza Virus Infection in Seven Cities Located in Guangdong Province, South China in 2010Journal of Animal and Veterinary Advances, 2012, 11(17), 3068-3072.
Effect of Additives on the Quality and in vitro Digestibility of Alfalfa Hay with High Moisture ContentJournal of Animal and Veterinary Advances, 2012, 11(17), 3094-3099.
Immunoregulation of Lycium barbarum Polysaccharide in Vaccinated ChickensJournal of Animal and Veterinary Advances, 2012, 11(17), 3105-3110.
Estimation of Genetic Parameters of Reproductive and Milk Yield Traits Using Multiple-Trait Animal Model in Holstein Under Subtropical ConditionsJournal of Animal and Veterinary Advances, 2012, 11(17), 3132-3139.
Preparation and Application of Polyclonal Antibody against PKZ from Ctenopharyngodon idellus (CiPKZ)Journal of Animal and Veterinary Advances, 2012, 11(17), 3169-3174.
Effects of Dietary Supplementation with Combined Arginine and Glutamine on Growth Performance and Small Intestinal Development in Neonatal PigletsJournal of Animal and Veterinary Advances, 2012, 11(17), 3187-3193.
Immunogenicity of Recombinant Attenuated Salmonella Choleraesuis C500 Strain Co-Expressing M and N Protein of Porcine Epidemic Diarrhea Virus (PEDV)Journal of Animal and Veterinary Advances, 2012, 11(17), 3234-3241.
Antibacterial Activity and Mechanism of Pogostemon cablin Against Bacteria from Milk of Dairy Cows Suffering with MastitisJournal of Animal and Veterinary Advances, 2012, 11(18), 3289-3297.
The Genetic Diversity of Hainan Black Goat by Capillary Gel Electrophoresis with Microsatellite Markers in Tropical Area of ChinaJournal of Animal and Veterinary Advances, 2012, 11(18), 3378-3384.
Comprehensive Therapy for Subfoveal Choroidal Neovascularization Secondary to Multifocal ChoroiditisJournal of Animal and Veterinary Advances, 2012, 11(18), 3396-3398.
Characterization of MicroRNAs in Paragonimus westermani by Solexa Deep Sequencing and Bioinformatics AnalysisJournal of Animal and Veterinary Advances, 2012, 11(18), 3469-3473.
Polymorphisms in Exon 2 of IGF-IR Gene and Theirs Association with Production Traits in Jinghai Yellow ChickenJournal of Animal and Veterinary Advances, 2012, 11(19), 3499-3505.
Rapid Detection of Virulent Salmonella Strains Using Multiplex PCRJournal of Animal and Veterinary Advances, 2012, 11(19), 3544-3549.
Effects of Dimethyl Sulfoxide, Ethylene Glycol, Propylene Glycol and Glycerol on Cryopreservation of Wild Tree Shrew (Tupaia belangeri Chinese) Cauda Epididymal SpermJournal of Animal and Veterinary Advances, 2012, 11(19), 3568-3574.
Associations of Very Low Density Lipoprotein Receptor (VLDLR) Gene Polymorphisms with Reproductive Traits in a Chinese Indigenous Chicken BreedJournal of Animal and Veterinary Advances, 2012, 11(19), 3662-3667.
The Effect of Ammonia and Humidity in Poultry Houses on Intestinal Morphology and Function of BroilersJournal of Animal and Veterinary Advances, 2012, 11(19), 3641-3646.
The Effect of Rearing Experience on the Behavior Patterns of Captive Male Alpine Musk DeerJournal of Animal and Veterinary Advances, 2011, 10(1), 128-132.
Clonal Integration for the L. chinensis in a Heterogeneous Alkaline Stress EnvironmentJournal of Animal and Veterinary Advances, 2012, 11(20), 3796-3799.
Prokaryotic Expression of p1 Gene of Yersinia ruckeri Isolated from Channel Catfish (Ictalunes punctatus) and Optimization of Expression ConditionsJournal of Animal and Veterinary Advances, 2012, 11(20), 3800-3805.
Advance in Proteomics Research and ApplicationJournal of Animal and Veterinary Advances, 2012, 11(20), 3812-3817.
Effect of Lactobacillin on the Mucosal Immune Function in Duodenum of Young BroilerJournal of Animal and Veterinary Advances, 2012, 11(20), 3843-3848.
Characterization of β-Actin Promoter of Leuciscus merzbacheriJournal of Animal and Veterinary Advances, 2012, 11(20), 3849-3854.
Impact of Calcium, Zinc and Copper Concentrations in Seminal Plasma on Semen Parameters in Zie GeeseJournal of Animal and Veterinary Advances, 2012, 11(20), 3840-3842.
Photosynthetic Properties of Different Drought Resistant Chicory Strains and their Responses to Drought StressJournal of Animal and Veterinary Advances, 2012, 11(21), 3898-3907.
The Characteristics of Context-Dependent Codon Usage Bias for Cleavage Sites in the Polyprotein of Foot and Mouth Disease VirusJournal of Animal and Veterinary Advances, 2012, 11(21), 3957-3968.
Clone and Bioinformatics Analysis of Chinese-Belgium Rabbit Metallothionein-1 (MT1), MT2 and MT3 Genes CDS RegionJournal of Animal and Veterinary Advances, 2012, 11(21), 4034-4039.
Effect of Mitochondrial Dysfunction in Nuclear Donor and Ooplasmic Recipient on Early Development of Goat-Sheep Interspecies Somatic Nuclear Transfer EmbryosJournal of Animal and Veterinary Advances, 2012, 11(22), 4079-4085.
Comparative Pharmacokinetics of Two Injection Formulations of Tulathromycin after a Single Intramuscular Administration in Healthy SwineJournal of Animal and Veterinary Advances, 2012, 11(22), 4201-4204.
First Report of Actinobacillus pleuropneumoniae Prevalence in Tibetan Pigs in Tibet, ChinaJournal of Animal and Veterinary Advances, 2012, 11(22), 4167-4170.
The Regulatory Role of Colorectal Adenocarcinoma Hypoxia-Inducible Factor-1α in the Expression of N-myc Down-Stream Regulative Gene 1Journal of Animal and Veterinary Advances, 2012, 11(22), 4121-4126.
Effect of Different Absorbents on Fermentation Quality of Wet Potato PulpJournal of Animal and Veterinary Advances, 2012, 11(22), 4230-4235.
Detection of Pathogenic Marek’s Disease Virus by Dot Blot Hybridization and PCR in Commercial Chickens in ChinaJournal of Animal and Veterinary Advances, 2012, 11(22), 4250-4257.
Expression Patterns of the Cannabinoid Receptor 1 Gene in Pig Skeletal Muscle and Skeletal Satellite Muscle CellsJournal of Animal and Veterinary Advances, 2012, 11(23), 4297-4303.
A Bibliometric Analysis of Peste Des Petits RuminantsJournal of Animal and Veterinary Advances, 2012, 11(23), 4389-4393.
Characteristics of Fluoroquinolone-Resistant of Avian Pathogenic Escherichia coli Isolates in ChinaJournal of Animal and Veterinary Advances, 2012, 11(23), 4406-4412.
Antioxidant Activity of Aspirin Eugenol Ester for Aging Model of Mice by D-GalactoseJournal of Animal and Veterinary Advances, 2012, 11(23), 4401-4405.
Development of Real-Time PCR Methods for the Detection of CD163 and Porcine Reproductive and Respiratory Syndrome Virus N Genes in Marc-145 CellJournal of Animal and Veterinary Advances, 2012, 11(23), 4489-4493.
Expression of Proliferation Cell Nuclear Antigen (PCNA) in Peripheral Erythrocytes of Triploid Rainbow Trout Oncorhynchus mykiss (Walbaum)Journal of Animal and Veterinary Advances, 2012, 11(23), 4451-4454.
The Effect of Helicobacter pylori on Apoptosis of the GES-1 Transfected pEGFP-N1-UreB Eukaryotic Expression Plasmid in vitroJournal of Animal and Veterinary Advances, 2012, 11(23), 4435-4440.
First Report of the Wild Tibetan Macaque (Macaca thibetana) as a New Primate Host of Gongylonema pulchrum with High Incidence in ChinaJournal of Animal and Veterinary Advances, 2012, 11(24), 4514-4518.
First Record of Chlamydia Seroprevalence in Peafowls in Southwestern ChinaJournal of Animal and Veterinary Advances, 2012, 11(24), 4567-4570.
Effect of Grazing Intensity on Microorganisms Quantity and Microbial Biomass of Soil in Grassland under Protection Forest of Songnen PlainJournal of Animal and Veterinary Advances, 2012, 11(24), 4549-4552.
Antimicrobial Susceptibility Testing of Mycoplasma bovis Isolated from Dairy Cows in Ningxia Hui Autonomous Region, Province of ChinaJournal of Animal and Veterinary Advances, 2012, 11(24), 4559-4562.
Somatic Cell Nuclear Transfer Efficiency Associated with Donor Cell Types in the Cashmere GoatJournal of Animal and Veterinary Advances, 2012, 11(24), 4578-4584.
Comparison of Effect of Non-Specific Filtering Methods and their Combinations on GeneChip Data Analysis: A Case for Affymetrix Porcine Genome MicroarrayJournal of Animal and Veterinary Advances, 2012, 11(24), 4593-4598.
Construction and Transfection of EGFP-N1-BDNF Expression PlasmidJournal of Animal and Veterinary Advances, 2012, 11(24), 4599-4602.
Cloning, Sequencing and Expression of a Heat Shock Protein 70 Gene from Tenebrio molitorJournal of Animal and Veterinary Advances, 2012, 11(24), 4632-4643.
Analysis of the Effect of Virulent Marek’s Disease Virus SORF2 Gene on its Horizontal Transmission Capacity Using Real-Time PCRJournal of Animal and Veterinary Advances, 2012, 11(24), 4648-4654.
Cytokine and Chemokine Microarray Profiles in Lung and Hilar Nodes from Pigs after Experimental Infection with Actinobacillus PleuropneumoniaeJournal of Animal and Veterinary Advances, 2012, 11(24), 4603-4610.
Responses in Milk Yield, Milk Composition and Rumen Fermentation in Lactating Cows Receiving a Corn Straw or Mixed Forage DietJournal of Animal and Veterinary Advances, 2012, 11(24), 4678-4683.
Therapeutic and Persistent Efficacy of Doramectin Against Nematode in Swine Infected Naturally in ChinaJournal of Animal and Veterinary Advances, 2012, 11(24), 4684-4688.
Cloning and Expression of Chicken AvBD8 Mature Peptide Gene in Lactococcus lactisJournal of Animal and Veterinary Advances, 2012, 11(23), 4369-4373.
Phylogenetic Analysis of the ORF2 Genes of Porcine Circovirus 2 Strains Isolated in Yunnan Province, ChinaJournal of Animal and Veterinary Advances, 2013, 12(1), 1-5.
Determination of Esptein-Barr Virus in the Plasma of Adult Patients with Otitis Media with EffusionJournal of Animal and Veterinary Advances, 2013, 12(1), 81-84.
In vitro Optimization of White Yak (Bos grunniens) Oviduct Epithelial Cells CultureJournal of Animal and Veterinary Advances, 2013, 12(1), 99-103.
Identification of Polymorphisms in the Bovine Paraoxonase 1 GeneJournal of Animal and Veterinary Advances, 2013, 12(2), 229-233.
Pilot Study on Degradation of Classical Swine Fever Virus Nonstructural 2 Protein in CellsJournal of Animal and Veterinary Advances, 2013, 12(2), 234-241.
Inflorescence Mutant Effect on Seed Yield Components in Alfalfa (Medicago sativa L. Gannong No. 6)Journal of Animal and Veterinary Advances, 2013, 12(3), 377-381.
Comparison of Ruminal and Rectal Sampling Sites for Estimating Digesta Passage Kinetics in Rumen of Holstein Cows Fed Corn Stalk-Based DietsJournal of Animal and Veterinary Advances, 2013, 12(9), 959-964.
Therapeutic Effect of Fructus Hordei Germinatus Ethanol Extract on Hyperplasia of Mammary Gland in RatsJournal of Animal and Veterinary Advances, 2013, 12(9), 954-958.
First Report of Neospora caninum Seroprevalence in Dairy Cattle in Jiangxi Province, Southeastern ChinaJournal of Animal and Veterinary Advances, 2013, 12(12), 1131-1133.
Phylogenetic Relationships of the 18SrRNA Gene Sequence of Histomonas meleagridis from Chickens in East ChinaJournal of Animal and Veterinary Advances, 2013, 12(16), 1338-1342.
Phylogenetic Analysis of the Streptococcus agalactiae Sip Gene in ChinaJournal of Animal and Veterinary Advances, 2013, 12(17), 1395-1398.
The Efficacy and Mechanism of Dexmedetomidine on the Myocardial Apoptosis Induced by Grave Scalding Rat ModelJournal of Animal and Veterinary Advances, 2014, 13(6), 377-384.
A hsp70 Gene-Based PCR for Detection of Mycoplasma ovipneumoniaeJournal of Animal and Veterinary Advances, 2013, 12(19), 1495-1497.
High Lysine-Yielding Bacillus subtilis as a Promising Alternative to Antibiotic for its Effects on Performance and Immune Responses of Linwu DucksJournal of Animal and Veterinary Advances, 2014, 13(6), 355-364.
Prevalence of Helminths in Adult Pigs in Hunan Province, ChinaJournal of Animal and Veterinary Advances, 2013, 12(12), 1123-1125.
Genetic Variants of the A-FABP Gene are Associated with Meat Production Traits in Different Cattle BreedsJournal of Animal and Veterinary Advances, 2014, 13(13), 792-796.
Studies of Sulfur-Induced Copper Deficiency in the Bactrian CamelJournal of Animal and Veterinary Advances, 2014, 13(14), 886-890.
Extraction of Alkaloids from C. komarovii Al. IljinskiJournal of Animal and Veterinary Advances, 2014, 13(15), 905-907.