Research Article
The Receptor for Bacillus Thuringiensis Cry 1Ac Delta-endotoxin in the Brush Border Membrane of the Lepidopteran Helicoverpa armigera is Aminopeptidase N

Kausar Malik, Tariq Mahmood and S. Riazuddin

Journal of Biological Sciences, 2001, 1(8), 782-784.


120 Kda and 70 Kda protein in the larval midgut membrane of the lepidopteran Helicoverpa armigera identified as putative receptor for Bt Cry 1Ac delta-endotoxin .Receptor proteins have been purified by a combination of gel-filtration and anion exchange chromatography .In ligand-blotting experiment ,the purified protein has binding capacity with Cry 1Ac and Cry 1Ab but not to Cry 2A .N-terminal sequence obtained from the protein show no homology to already existing sequences .When assayed for amino-peptidase and alkaline phosphatase activities , purified receptors preparations were enriched 2.5 fold in amino-peptidase activity compare to Helicoverpa-armigera brush border membrane vesicles . The 70 Kda protein seems to be the part of 120 Kda receptor protein.

ASCI-ID: 38-346

Cited References Fulltext

Similar Articles

Evaluation of Adjuvants for Increased Efficacy of HearNPV Against Helicoverpa armigera (Hubner) Using Suntest Machine

Journal of Biological Sciences, 2008, 8(3), 534-541.

Monitoring and Control of Helicoverpa armigera by Synthetic Pheromone Traps in Peas, Pisum sativum

Journal of Biological Sciences, 2003, 3(10), 926-931.

Synergistic Effect of Bacillus thuringiensis subsp. aizawai with Synthetic Pyrethroids against Insecticide Resistant Helicoverpa armigera (Hubner)

Journal of Biological Sciences, 2005, 5(6), 678-680.

Stimulo-deterrent Diversionary Strategy with Conjunctive Use of Trap Crops, Neem and Bacillus thuringiensis Berliner for the Management of Insecticide Resistant Helicoverpa armigera (Hubner) in Cotton

Journal of Biological Sciences, 2005, 5(6), 681-686.

Cited By

Interaction of Allium sativum leaf agglutinin with midgut brush border membrane vesicles proteins and its stability in Helicoverpa armigera

PROTEOMICS, 2010, (), n/a. DOI: 10.1002/pmic.201000152

The use of CAMB biopesticides to control pests of rice (Oryza sativa).

Asian Journal of Plant Sciences, 2003, 2(15), 1079. DOI: 10.3923/ajps.2003.1079.1082

Diversity of aminopeptidases, derived from four lepidopteran gene duplications, and polycalins expressed in the midgut of Helicoverpa armigera: Identification of proteins binding the δ-endotoxin, Cry1Ac of Bacillus thuringiensis

Insect Biochemistry and Molecular Biology, 2008, 38(7), 685. DOI: 10.1016/j.ibmb.2008.03.010

Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters

Toxins, 2016, 8(12), 79. DOI: 10.3390/toxins8030079