Research Article 
The
Receptor for Bacillus Thuringiensis Cry 1Ac Delta-endotoxin in the Brush
Border Membrane of the Lepidopteran Helicoverpa armigera is Aminopeptidase
N
Kausar Malik, Tariq Mahmood and S. Riazuddin
Journal of Biological Sciences, 2001, 1(8), 782-784.
Abstract
120 Kda and 70 Kda protein in the larval midgut membrane of the lepidopteran Helicoverpa armigera identified as putative receptor for Bt Cry 1Ac delta-endotoxin .Receptor proteins have been purified by a combination of gel-filtration and anion exchange chromatography .In ligand-blotting experiment ,the purified protein has binding capacity with Cry 1Ac and Cry 1Ab but not to Cry 2A .N-terminal sequence obtained from the protein show no homology to already existing sequences .When assayed for amino-peptidase and alkaline phosphatase activities , purified receptors preparations were enriched 2.5 fold in amino-peptidase activity compare to Helicoverpa-armigera brush border membrane vesicles . The 70 Kda protein seems to be the part of 120 Kda receptor protein.
ASCI-ID: 38-346
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