Research Article 
Effect of Seed Cleaning and Washing on the Incidence of Bacterial Leaf Blight of Rice
A.K.M. Anowarul Hoque, M. R. Alam, Md. Nazrul Islam, M.K. Anam, M. Asad-ud-doullah and M.M. Hossain
Journal of Biological Sciences, 2002, 2(9), 565-568.
Abstract
The study was conducted to determine the effect of seed cleaning and washing on the incidence of bacterial leaf blight (BLB) of rice cv. BR11. Six different treatments were used. They were farmer`s seeds clean seeds, diseased seeds, farmer`s seeds washed with tap water, farmer`s seeds washed with 20% brine solution and clean seeds washed with tap water. Three seed health testing methods namely paper rolled towel, cassette slide holder and liquid assay method were used for detection of BLB infection in seed. Seed health both before sowing and freshly harvested seeds from six different treatments were determined. Seedling vigour of clean seeds and brine solution washed seed were also higher than farmer`s seed and diseased seed. Xanthomonas oryzae pv. oryzae was identified through physiological, biological, serological, hypersensitive reaction and pathogencity tests in seeds of all treatments. Incidence of BLB in field plots were recorded at tillering stage and flag leaf stage. Maximum and minimum BLB disease was recorded in diseased seeds and brine solution washed seeds respectively for both situation.
ASCI-ID: 38-605
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