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Research Article
Callus Induction and Regeneration from Nodal Segment of Potato Cultivar Diamant

N. Khatun, M.A. Bari, R. Islam, S. Huda, N.A.Siddique, M.H. Rahman and M.U. Mollah

Journal of Biological Sciences, 2003, 3(12), 1101-1106.

Abstract

The nodal segments of Diamant cultivar of potato from in vitro grown plantlets were cultured for callus induction and regeneration on MS semisolid medium supplemented with different concentrations of 2,4-D, NAA, BAP alone and NAA with BAP. Highest 90.00% of callus formation was observed in MS+2.5 mgl G 12,4-D. The second highest 83.33% callus induction was recorded in MS+5.0 mgl G 1 BAP. Maximum percentages (70.00%) of calli-induced shoots were observed in MS medium fortified with 5.0 mgl G 1 BAP+0.1 mgl G 1IBA. The regenerated shoots were rooted on MS and 2 MS medium containing different concentrations of IBA and maximum rooting response was achieved in 2 MS +1.0 mgl G 1IBA. Regenerated plants were successfully established in soil after acclimatization.

ASCI-ID: 38-810

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