Clonal Propagation of White Mulberry (Morus alba L.) Using in vitro Technique

Research Article
Clonal Propagation of White Mulberry (Morus alba L.) Using in vitro Technique

A. Habib, M.R. Ali, M.N. Amin and M.M. Rahman

Journal of Biological Sciences, 2003, 3(12), 1181-1187.

Abstract

The present study was undertaken with a view to develop a reproducible protocol for in vitro propagation of white mulberry (Morus alba L.). For this purpose, surface sterilization of the explants from field grown mature plants was done with 0.1% HgCl₂ with a treatment duration of 5 minutes for shoot tip and that with 6 minutes for nodal segments. Between the two explants used, nodal explant exhibit comparatively better response to axillary shoot proliferation. Analysis of the results obtained from the proliferation experiment proved that effectiveness of cytokinin BA was better than that of Kn with respect to axillary shoot formation. And that MS medium supplemented with 1.0 mg l G ¹ BA was found to be best where 100% of the explants proliferated with the axillary shoots having average length of 5.46 " 0.02 cm and a multiplication rate of 7 fold per 4-week. On the other hand, percentage of root induction and number of roots per shoot were largely affected by the concentration of MS medium and type of the auxins used. The highest percentage (100%) of root regeneration was obtained in half MS medium supplemented with 0.5% mg l G ¹ IBA.

ASCI-ID: 38-820

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