Research Article 
Monitoring the Effect of Insecticide Selection on Culex pipiens (Diptera:
Culicidae) Larval Susceptibility to Malathion and Lambda-Cyhalothrin
El-Sayed A. El-Sheikh, Mohamed-Bassem A. Ashour, Mohamed M. Aamir and Mohamed M. Gamal
Journal of Entomology, 2014, 11(1), 14-24.
Abstract
The susceptibility of Culex pipiens collected from different localities of the Sharkia Governorate, Egypt, to malathion and lambda-cyhalothrin was investigated for 11 successive generations. Larval Cx. pipiens developed 57 and 305-fold resistance to malathion and lambda-cyhalothrin, respectively after 11 successive generations of selection pressure. The susceptibility of unselected generations, due to non-exposure to both insecticides for 11 generations, was increased to 1.1-fold in F11 for malathion and to 1.5-fold in F11 for lambda-cyhalothrin. Acetylcholinestrase (AChE) activity increased gradually in both selected and unselected generations until the 11th generation. The activity of AChE in generations (F1-F7) selected with malathion was significantly lower than that of the lambda-cyhalothrin selected and unselected generations. General esterase activity increased 3.7 and 3.0-fold (malathion) and 4.2 and 3.6-fold (lambda-cyhalothrin) compared with the susceptible strain (LS-CP), when either α-napthyl acetate (NA) or β-NA were used as general substrates in F11 selected generations, respectively. A significant increase in glutathione-S-transferase (GST) activity was noticed in the F11 generation recording 6400 and 8800 μmoL min-1 mg-1 protein for malathion-and lambda-cyhalothrin-selected generations, respectively. The ratios recorded in the 11th generations were 3.6 and 4.9 fold as compared with LS-CP for malathion and lambda-cyhalothrin selected generations, respectively. Our results indicate that the Cx. pipiens mosquito strain from Egypt can increase resistance to malathion and lambda-cyhalothrin if these insecticides are continuously or rotationally used to control this species. Increased resistance is likely to be associated with increased activity of target and metabolic enzyme systems.
ASCI-ID: 48-388
Similar Articles
Oviposition Deterrent Activity of Some Volatile Oils against the Filaria Mosquito Vector Culex pipiens
Journal of Entomology, 2012, 9(6), 435-441.
Mortality and GST Enzyme Response of Saw-toothed Grain Beetles, Oryzaephilus surinamensis (Coleoptera: Silvanidae) Exposed to Low Insecticide ConcentrationsJournal of Entomology, 2012, 9(6), 396-402.
Susceptibility and Biochemical Determination of House Fly (Musca domestica L.) to Lambda-cyhalothrinJournal of Entomology, 2019, 16(3), 91-97.
Antioxidant Defense of Apis mellifera L. in Response to Chlorophyllin Derivatives: As a Marker of Ecotoxicological StressJournal of Entomology, 2022, 19(1), 9-19.
Susceptibility and Biochemical Determination of House Fly (Musca domestica L.) to Lambda-cyhalothrinJournal of Entomology, 2019, 16(3), 91-97.