Search. Read. Cite.

Easy to search. Easy to read. Easy to cite with credible sources.

Research Article
Inhibitory Effects of Bruceine A Biolarvicide on Growth and Development of Aedes aegypti Larvae

Dwi Sutiningsih, Mustofa, Tri Baskoro Tunggul Satoto and Edhi Martono

Journal of Entomology, 2017, 14(2), 104-111.

Abstract

Background and Objective: Bruceine A is a quassinoid compound isolated from the seeds of Makassar fruit (Brucea javanica L. Merr.) that has a biolarvicidal effect toward Aedes aegypti larvae with a lethal concentration fifty (LC50) and ninety (LC90) of 0.408 and 4.923 ppm, respectively. This study aimed to demonstrate the inhibitory effect of bruceine A on the growth and development of A. aegypti larvae at sublethal concentrations. Materials and Methods: The effect of bruceine A on A. aegypti larvae growth and development was tested using bioassays. Daily counts of the number of larvae that survived or died were taken until all the experimental larvae became either pupae or adults. Dead larvae were assessed for morphological and histological changes. The statistical analysis of growth and development inhibition was performed by using Kruskal Wallis test followed by Mann Whitney. The data from observation on morphological and histological changes were descriptively analyzed. Results: Inhibitory effects of bruceine A on the growth and development of A. aegypti larvae were seen at concentrations as low as 0.01 ppm. The morphological changes seen in larvae included damage to the gastrointestinal tract manifested as black spots, folded breathing tubes and detached cuticle and setae as well as smaller body sizes compared to the control group. Histological examination of the larvae indicated diffuse necrosis in the gastrointestinal epithelium accompanied by epithelial cell shrinkage, whereas the core was not visible. Conclusion: It is concluded that bruceine A inhibits growth and development of A. aegypti larvae, as shown by cuticle damage and necrosis of gastrointestinal and respiratory epithelial cells.

ASCI-ID: 48-465

Cited References Fulltext

Related Articles


Reduction in Haemocyte Mediated Immune Response in Danais chrysippus Following Treatment with Neem-Based Insecticides

Journal of Entomology, 2008, 5(3), 200-206.

Assessment on Biological and Morphometric Parameters of Jatropha Leaf Webber Cum Fruit Webber, Pempelia morosalis (Saalm Uller) along with Associated Natural Enemies in Eastern Uttar Pradesh of India

Journal of Entomology, 2011, 8(1), 88-94.

Effects of Tramadol on the Development of Lucilia sericata (Diptera: Calliphoridae) and Detection of the Drug Concentration in Postmortem Rabbit Tissues and Larvae

Journal of Entomology, 2011, 8(4), 353-364.

Efficiency of Azadirachtin, A Chitin Synthesis Inhibitor on Growth, Development and Reproductive Potential of Tribolium confusum after Adult Treatment

Journal of Entomology, 2011, 8(5), 440-449.

Development and Reproduction of Bemisia tabaci on Three Tomato Varieties

Journal of Entomology, 2007, 4(3), 231-236.

Toxicity and Growth-Disruptive Effects of Silica, Zinc and Aluminum Oxide Nanoparticles on Spiny Bollworm, Earias insulana

Journal of Entomology, 2021, 18(1), 8-18.

Assessment on Biological and Morphometric Parameters of Jatropha Leaf Webber Cum Fruit Webber, Pempelia morosalis (Saalm Uller) along with Associated Natural Enemies in Eastern Uttar Pradesh of India

Journal of Entomology, 2011, 8(1), 88-94.

Effects of Net Barrier and Synthetic Insecticides on Phytolyma lata (Homoptera: Psyllidae) Infestation, Growth and Survival of Milicia excelsa in the Field

Journal of Entomology, 2014, 11(4), 218-224.

Toxicity and Growth-Disruptive Effects of Silica, Zinc and Aluminum Oxide Nanoparticles on Spiny Bollworm, Earias insulana

Journal of Entomology, 2021, 18(1), 8-18.

Inhibition of Larvae and Adult α-amylase in Xanthogaleruca luteola Muller (Col.: Chrysomelidae) by Cereal and Legumes Seed Proteinaceous Extracts

Journal of Entomology, 2016, 13(5), 179-186.